Chromosome Numbers of Rubus Species at the National Clonal Germplasm Repository

Thompson, Maxine M.

doi:10.21273/hortsci.30.7.1447

Cited by 88 publications

(48 citation statements)

References 32 publications

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“…The three R. coreanus populations were all diploid, same as the previous reports by Pool et al (1981), Naruhashi (1991, 1998), Thompson and Zhao (1993) and Thompson (1995). Karyotypes of the three R. coreanus populations were all extremely similar and can be expressed as 2n = 29 = 14 = 12 m (2SAT) ?…”

Section: Description Of Karyotpessupporting

confidence: 83%

“…It is great hopeful for selection of the type with fewer seeds from the triploid. Although polyploids are not known to be common in wild raspberry germplasm (Thompson 1995(Thompson , 1997, our triploid R. parvifolius population with reduced seed production may be very useful for seedless raspberry breeding, if the trait is not just the result of a cytological abnormality and can be transferred to commercial cultivars.…”

Section: Discussionmentioning

confidence: 99%

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Cytological and RAPD data revealed genetic relationships among nine selected populations of the wild bramble species, Rubus parvifolius and R. coreanus (Rosaceae)

Wang

Liu

BiFeng

et al. 2009

Genet Resour Crop Evol

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Rubus parvifolius L. and R. coreanus Miq. are the two morphologically distinct, endemic wild brambles in East Asia that may be useful in raspberry breeding and cultivar development. In this study, genetic relationships among a total of nine populations of these two species, collected from Sichuan Province in southwestern China, were investigated by cytological and RAPD analyzing. With the exception of ploidy levels, R. parvifolius and R. coreanus had similar cytological features. Six R. parvifolius populations included diploids, triploid, tetraploid, and mixed diploid-tetraploid cytotype plants; among them, the population that produced the fewest seeds was the triploid, while the three R. coreanus populations were all diploid. But obvious genetic differentiation between R. parvifolius and R. coreanus was revealed by RAPD markers, the Euclidean's GS coefficient generated by UPGMA cluster analysis between R. parvifolius and R. coreanus was only 0.442, about half of that observed within species. Both genetic relationships among these populations and the origin of polyploidy in R. parvifolius populations are also discussed.

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Section: Description Of Karyotpessupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Cytological and RAPD data revealed genetic relationships among nine selected populations of the wild bramble species, Rubus parvifolius and R. coreanus (Rosaceae)

Wang

Liu

BiFeng

et al. 2009

Genet Resour Crop Evol

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“…& Schltdl., and R. laciniatus Willd., both of subgenus Rubus (Thompson 1995b). Sub-family Rosoideae is sister to Spiraeoideae, to which Malus (apple) and Prunus (peach and other stonefruits) belong (Potter et al 2007) (Fig.…”

Section: Introductionmentioning

confidence: 99%

Construction of black (Rubus occidentalis) and red (R. idaeus) raspberry linkage maps and their comparison to the genomes of strawberry, apple, and peach

Bushakra

Stephens²,

Atmadjaja

et al. 2012

Theor Appl Genet

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The genus Rubus belongs to the Rosaceae and is comprised of 600-800 species distributed world-wide. To date, genetic maps of the genus consist largely of non-transferable markers such as amplified fragment length polymorphisms. An F(1) population developed from a cross between an advanced breeding selection of Rubus occidentalis (96395S1) and R. idaeus 'Latham' was used to construct a new genetic map consisting of DNA sequence-based markers. The genetic linkage maps presented here are constructed of 131 markers on at least one of the two parental maps. The majority of the markers are orthologous, including 14 Rosaceae conserved orthologous set markers, and 60 new gene-based markers developed for raspberry. Thirty-four published raspberry simple sequence repeat markers were used to align the new maps to published raspberry maps. The 96395S1 genetic map consists of six linkage groups (LG) and covers 309 cM with an average of 10 cM between markers; the 'Latham' genetic map consists of seven LG and covers 561 cM with an average of 5 cM between markers. We used BLAST analysis to align the orthologous sequences used to design primer pairs for Rubus genetic mapping with the genome sequences of Fragaria vesca 'Hawaii 4', Malus × domestica 'Golden Delicious', and Prunus 'Lovell'. The alignment of the orthologous markers designed here suggests that the genomes of Rubus and Fragaria have a high degree of synteny and that synteny decreases with phylogenetic distance. Our results give unprecedented insights into the genome evolution of raspberry from the putative ancestral genome of the single ancestor common to Rosaceae.

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“…Rubus species and cultivars are clonally propagated and maintained in greenhouses, screenhouses, field collections, and as tissue-cultured plants and cryopreserved shoot tips (Gupta and Reed 2006). The NCGR Rubus collection consists of 2,094 accessions representing 193 species from 64 countries and is a unique source of genetically diverse genotypes for use by plant breeders and scientists across the world (Thompson 1995).…”

Section: Introductionmentioning

confidence: 99%

Genetic stability of cryopreserved shoot tips of Rubus germplasm

Castillo

Bassil

Wada

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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Questions often arise concerning the genetic stability of plant materials stored in liquid nitrogen for long time periods. This study examined the genetic stability of cryopreserved shoot tips of Rubus germplasm that were stored in liquid nitrogen for more than 12 yr, then rewarmed and regrown. We analyzed the genetic stability of Rubus grabowskii, two blackberry cultivars ("Hillemeyer" and 'Silvan'), and one raspberry cultivar ("Mandarin") as in vitro shoots and as field-grown plants. No morphological differences were observed in greenhouse-grown cryopreserved plants when compared to the control mother plants. In the field, cryopreserved plants appeared similar but were more vigorous than mother plants, with larger leaves, fruit, and seeds. Single sequence repeats (SSR) and amplified fragment length polymorphism (AFLP) analyses were performed on shoots immediately after recovery from cryopreservation and on shoots subcultured for 7 mo before analysis. Ten SSR primers developed from "Marion" and "Meeker" microsatellite-enriched libraries amplified one to 15 alleles per locus, with an average of seven alleles and a total of 70 alleles in the four genotypes tested. No SSR polymorphisms were observed between cryopreserved shoots and the corresponding mother plants regardless of subculture. Although no polymorphisms were detected in shoots analyzed immediately after recovery from cryopreservation, AFLP polymorphisms were detected in three of the four Rubus genotypes after they were subcultured for 7 mo. Field-grown plants from the polymorphic shoot tips of R. grabowskii and 'Silvan' displayed the same AFLP fingerprints as their corresponding mother plants. Only longcultured in vitro shoot tips displayed polymorphisms in vitro, and they were no longer detected when the plants were grown ex vitro. The transitory nature of these polymorphisms should be carefully considered when monitoring for genetic stability.

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Chromosome Numbers of Rubus Species at the National Clonal Germplasm Repository

Cited by 88 publications

References 32 publications

Cytological and RAPD data revealed genetic relationships among nine selected populations of the wild bramble species, Rubus parvifolius and R. coreanus (Rosaceae)

Cytological and RAPD data revealed genetic relationships among nine selected populations of the wild bramble species, Rubus parvifolius and R. coreanus (Rosaceae)

Construction of black (Rubus occidentalis) and red (R. idaeus) raspberry linkage maps and their comparison to the genomes of strawberry, apple, and peach

Genetic stability of cryopreserved shoot tips of Rubus germplasm

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