Chromosome painting to locate genes for drought resistance transferred from Festuca arundinacea into Lolium multiflorum

Humphreys, M. W.; Pašakinskiené, Izolda

doi:10.1038/hdy.1996.180

Cited by 61 publications

(46 citation statements)

References 9 publications

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“…However, the significance of the Fp introgressions reported here is greater, as Lm is more sensitive to freezing conditions than L. perenne and the benefits to accrue from the Fp introgressions reported herein are therefore more significant. Despite freezing tolerance being a quantitative character governed by many genes, it is demonstrated here, as was reported previously for drought resistance (Humphreys and Pašakinskienė , 1996;Humphreys et al, 2005), that a single Festuca introgression can make a significant impact on the resistance of Lolium against abiotic stresses.…”

Section: Discussionsupporting

confidence: 75%

“…The development of the genomic in situ hybridisation (GISH) technique for cytogenetic analyses in Lolium-Festuca hybrids has represented a major advance in the genome analysis of these genera (eg Bailey et al, 1993;Thomas et al, 1994;Humphreys and Pašakinskienė , 1996;Zwierzykowski et al, 1998;Leśniewska et al, 2001;Pašakinskienė and Jones, 2005). GISH enables the parental chromosomes of Lolium and Festuca to be distinguished, and the sites of any genome recombination identified.…”

Section: Introductionmentioning

confidence: 99%

“…GISH enables the parental chromosomes of Lolium and Festuca to be distinguished, and the sites of any genome recombination identified. GISH also enables the monitoring of the introgression of alien chromatin from one species to another in different generations during backcross breeding programmes (eg Humphreys and Pašakinskienė , 1996;Zwierzykowski et al, 1998Zwierzykowski et al, , 1999.…”

Section: Introductionmentioning

confidence: 99%

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GISH/FISH mapping of genes for freezing tolerance transferred from Festuca pratensis to Lolium multiflorum

et al. 2006

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Section: Discussionsupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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GISH/FISH mapping of genes for freezing tolerance transferred from Festuca pratensis to Lolium multiflorum

et al. 2006

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“…Derivatives of an equivalent backcrossing programme were identified as having enhanced drought resistance, in some cases equal to F. arundinacea (Humphreys & Thomas, 1993). For studies of water use traits, we used two elite drought resistant Lolium genotypes with genes for drought resistance introgressed trom r. arundinacea and previously mapped to chromosome 2 of L. multifiorum (Humphreys & Pasakinskiene, 1996).…”

Section: Methodsmentioning

confidence: 99%

Dissecting drought‐ and cold‐tolerance traits in the Lolium–Festuca complex by introgression mapping

et al. 1997

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Recent advances in the cytogenetics of the Lolium?Festuca complex provide new opportunities for understanding and manipulating complex physiological mechanisms such as drought and cold resistance. This paper describes breeding programmes involving hybrids between two species: L. multiflorum, which offers good early growth and high nutritive value, and F. arundinacea, which is more stress tolerant. The programmes are designed to allow a rapid recovery of the Lolium genome and to restrict numbers of recombinants derived from Festuca. Use of genomic in situ hybridization (GISH) and an isozyme marker demonstrates how gene complexes from any part of the Festuca genome can be introgressed into Lolium. This enables us to construct genotypes combining desirable traits of both Lolium and Festuca species. By introgressing different Festuca genes into Lolium, quantitative traits such as tolerance to drought and cold can be ?dissected? into their different components, to clarify their function. Festuca genes for stress tolerance can be located by genomic in situ hybridization (GISH) and assigned to regions of chromosome arms in Lolium. Two Lolium genotypes are described, in which genes for drought resistance transferred from the F. pratensis sub-genome of F. arundinacea onto chromosome 2 of Lolium. The two drought-resistant lines have the high water conductance of Festuca on their adaxial leaf surface and the low abaxial conductance of Lolium. The paper also describes how androgenesis of L. multiflorum?F. arundinacea hybrids has led to the selection and characterization of genotypes with coacclimation to drought and freezing stress, in some cases exceeding that in the Festuca parent.Peer reviewe

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“…The potential use of Lolium/Festuca introgression mapping to develop physical and genetic maps, determine the genetic control of important characters and develop new germplasm for plant breeders has been discussed previously (King et al, 1998). Using this technique, it has been possible to identify a segment of chromosome from F. arundinacea introgressed into Lm and carrying drought tolerance (Humphreys and Pasakinskiene, 1996). It should be possible to identify the region of the Fp chromosome carrying genes for other traits, such as disease resistance.…”

Section: Introductionmentioning

confidence: 99%

Introgression of crown rust (Puccinia coronata) resistance from meadow fescue (Festuca pratensis) into Italian ryegrass (Lolium multiflorum) and physical mapping of the locus

et al. 2003

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Resistance was found in the meadow fescue (Festuca pratensis) to crown rust (Puccinia coronata), originating from ryegrasses (Lolium spp). A backcrossing programme successfully transferred this resistance into diploid Italian ryegrass (Lolium multiflorum) and genomic in situ hybridisation (GISH) was used to identify the introgressed fescue chromosome segment. The resistant (R) plants in two BC 3 lines all carried an introgressed segment on a single chromosome, which in one of the lines was confined to the short arm of the chromosome. Susceptible (S) plants either contained no introgressed chromosome segment or a segment which was physically smaller than the segments in resistant plants. Using GISH the resistance locus could be physically mapped to the midpoint of a short arm. Segregation ratios of the progeny of BC 3 plants, when crossed as R Â S and R Â R, were in agreement with the hypothesis that the resistance was controlled by a single gene or very closely linked genes. No R plants were produced by crossing S Â S plants.

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Chromosome painting to locate genes for drought resistance transferred from Festuca arundinacea into Lolium multiflorum

Cited by 61 publications

References 9 publications

GISH/FISH mapping of genes for freezing tolerance transferred from Festuca pratensis to Lolium multiflorum

GISH/FISH mapping of genes for freezing tolerance transferred from Festuca pratensis to Lolium multiflorum

Dissecting drought‐ and cold‐tolerance traits in the Lolium–Festuca complex by introgression mapping

Introgression of crown rust (Puccinia coronata) resistance from meadow fescue (Festuca pratensis) into Italian ryegrass (Lolium multiflorum) and physical mapping of the locus

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