Chromosome-scale genome assembly ofTinospora sagittata(Oliv.) Gagnep. enhances identifying genes involved in the biosynthesis of jatrorrhizine

Abstract

Tinospora sagittata (Oliv.) Gagnep. is an important medicinal tetraploid plant in the Menispermaceae family. Its tuber, namely "Radix Tinosporae" used in Traditional Chinese Medicine, is rich in medicinal terpenoids and benzylisoquinoline alkaloids (BIAs), To enhance understanding the biosynthesis of medicinal compounds, we, herein, report the assembly of a high quality chromosome-scale genome with both PacBio HiFi and Illumina sequencing technologies. The size of assembled genome was 2.33 Gb consisting of 4070 scaffolds (N50=42.06Mb), of which 92.05% were assigned to 26 pseudochromosomes in A and B sub-genomes. A phylogenetic analysis with the T. sagittata and other 16 plant genomes estimated the evolutionary placement of T. sagittata and its divergence time in Ranunculales. Further genome evolution analysis characterized one round tandem duplication about 1.5 million years ago (MYA) and one whole-genome duplication (WGD) about 86.9 MYA. WGD contributed to the duplication of clade-specific cytochrome P450 gene family in Ranunculales. Moreover, sequencing mining obtained genome-wide genes involved in the biosynthesis of alkaloids and terpenoids. TsA02G014550, one candidate, was functionally characterized to catalyze the formation of (S)-canadine in the jatrorrhizine biosynthetic pathway. Taken together, the assembled genome of T. sagittata provides useful sequences to understand the biosynthesis of jatrorrhizine and other BIAs in plants.

A comprehensive review on the progress and challenges of tetrahydroisoquinoline derivatives as a promising therapeutic agent to treat Alzheimer's disease

“…Jatrorrhizine is a type of protoberberine derived from plants. It has been widely recognized as a key bioactive moiety in various plant families, including Tinospora sagittate [ 43 ], Corydalis yanhusuo [ 44 ], Mahonia bealei [ 45 ], Coptis Chinensis Franch [ 46 ], and Berberis vernae [ 47 ]. The IUPAC name of jatrorrhizine is 2,9,10-trimethoxy-5,6-dihydroisoquinolino[2,1- b ]isoquinolin-7-ium-3-ol (molecular formula: C 20 H 20 NO 4 ) [ 48 ].…”