Chromosome-specific microsatellite multiplex sets for linkage studies in the domestic dog

Clark, Leigh Anne; Tsai, Kate L.; Steiner, Jörg M.; Williams, David A.; Guerra, Trina; Ostrander, Elaine A.; Galibert, Francis; Murphy, Keith E.

doi:10.1016/j.ygeno.2004.06.006

Cited by 47 publications

(46 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fluorescently labeled primers were synthesized and multiplex PCR was performed for Minimal Screening Set 2 markers as described by Clark et al (21). PCR products were resolved with an internal size standard (GeneScan 500 LIZ, Applied Biosystems) by using an ABI 3730xl DNA Analyzer (Applied Biosystems).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Retrotransposon insertion in SILV is responsible for merle patterning of the domestic dog

Clark

Wahl

Rees

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

227

242

View full text Add to dashboard Cite

Merle is a pattern of coloring observed in the coat of the domestic dog and is characterized by patches of diluted pigment. This trait is inherited in an autosomal, incompletely dominant fashion. Dogs heterozygous or homozygous for the merle locus exhibit a wide range of auditory and ophthalmologic abnormalities, which are similar to those observed for the human auditory-pigmentation disorder Waardenburg syndrome. Mutations in at least five genes have been identified as causative for Waardenburg syndrome; however, the genetic bases for all cases have not been determined. Linkage disequilibrium was identified for a microsatellite marker with the merle phenotype in the Shetland Sheepdog. The marker is located in a region of CFA10 that exhibits conservation of synteny with HSA12q13. This region of the human genome contains SILV, a gene important in mammalian pigmentation. Therefore, this gene was evaluated as a candidate for merle patterning. A short interspersed element insertion at the boundary of intron 10͞exon 11 was found, and this insertion segregates with the merle phenotype in multiple breeds. Another finding was deletions within the oligo(dA)-rich tail of the short interspersed element. Such deletions permit normal pigmentation. These data show that SILV is responsible for merle patterning and is associated with impaired function of the auditory and ophthalmologic systems. Although the mutant phenotype of SILV in the human is unknown, these results make it an intriguing candidate gene for human auditory-pigmentation disorders.short interspersed element ͉ pigmentation ͉ linkage disequilibrium

show abstract

Section: Methodsmentioning

confidence: 99%

“…To identify a chromosomal region segregating with merle, we carried out a whole-genome scan for the Shetland Sheepdog by using the multiplexed Minimal Screening Set 2 (20,21). Linkage disequilibrium (LD) for merle was identified with a microsatellite marker in a region of CFA10 that exhibits conservation of synteny with HSA12q13.…”

mentioning

confidence: 99%

Retrotransposon insertion in SILV is responsible for merle patterning of the domestic dog

Clark

Wahl

Rees

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

227

242

View full text Add to dashboard Cite

show abstract

“…Genotyping, statistical analysis, and genomics: Genotyping for the minimal screening set I panel of simple sequence length polymorphism (SSLP) repeat markers described by Richman et al (2001) was carried out using multiplex PCR as previously described (Cargill et al 2002;Clark et al 2004). Fluorescently labeled PCR products were separated on the automated laser fluorescence DNA sequencer ABI377 (PerkinElmer, Norwalk, CT), using GENESCAN (version 2.1) fragment analysis software, and alleles were identified using the GENOTYPER program (version 2.0; Perkin-Elmer).…”

Section: Methodsmentioning

confidence: 99%

Linkage and Segregation Analysis of Black and Brindle Coat Color in Domestic Dogs

et al. 2007

Self Cite

View full text Add to dashboard Cite

Mutations of pigment type switching have provided basic insight into melanocortin physiology and evolutionary adaptation. In all vertebrates that have been studied to date, two key genes, Agouti and Melanocortin 1 receptor (Mc1r), encode a ligand-receptor system that controls the switch between synthesis of red-yellow pheomelanin vs. black-brown eumelanin. However, in domestic dogs, historical studies based on pedigree and segregation analysis have suggested that the pigment type-switching system is more complicated and fundamentally different from other mammals. Using a genomewide linkage scan on a Labrador 3 greyhound cross segregating for black, yellow, and brindle coat colors, we demonstrate that pigment type switching is controlled by an additional gene, the K locus. Our results reveal three alleles with a dominance order of black (K B ) . brindle (k br ) . yellow (k y ), whose genetic map position on dog chromosome 16 is distinct from the predicted location of other pigmentation genes. Interaction studies reveal that Mc1r is epistatic to variation at Agouti or K and that the epistatic relationship between Agouti and K depends on the alleles being tested. These findings suggest a molecular model for a new component of the melanocortin signaling pathway and reveal how coat-color patterns and pigmentary diversity have been shaped by recent selection.

show abstract

“…Generally, 300-500 markers spanning the genome at a 5-10 centimorgan (cM) density are used for an initial genome wide scan 19,20,51 . Genetic markers are places in the genome where there is variation, or polymorphism, between alleles.…”

Section: Mapping Disease Causing Locimentioning

confidence: 99%

Finding cardiovascular disease genes in the dog

Parker

Meurs

Ostrander

2006

Journal of Veterinary Cardiology

Self Cite

View full text Add to dashboard Cite

Recent advances in canine genomics are changing the landscape of veterinary biology, and by default, veterinary medicine. No longer are clinicians locked into traditional methods of diagnoses and therapy. Rather major advances in canine genetics and genomics from the past five years are now changing the way the veterinarian of the 21 st century practices medicine.First, the availability of a dense genome map gives canine genetics a much needed foothold in comparative medicine, allowing advances made in human and mouse genetics to be applied to companion animals. Second, the recently released 7.5x whole genome sequence of the dog is facilitating the identification of hereditary disease genes. Finally, development of genetic tools for rapid screening of families and populations at risk for inherited disease means that the cost of identifying and testing for disease loci will significantly decrease in coming years.Out of these advances will come major changes in companion animal diagnostics and therapy. Clinicians will be able to offer their clients genetic testing and counseling for a myriad of disorders. Such advances are certain to generate healthier and more long lived dogs, improving quality of life for owner and pet alike. The clinician of the 21 st century, therefore, faces incredible opportunities as well as challenges in the management of genetic disease. In this review we summarize recent findings in canine genomics and discuss their application to the study of canine cardiac health.

show abstract

Chromosome-specific microsatellite multiplex sets for linkage studies in the domestic dog

Cited by 47 publications

References 9 publications

Retrotransposon insertion in SILV is responsible for merle patterning of the domestic dog

Retrotransposon insertion in SILV is responsible for merle patterning of the domestic dog

Linkage and Segregation Analysis of Black and Brindle Coat Color in Domestic Dogs

Finding cardiovascular disease genes in the dog

Contact Info

Product

Resources

About