Karyology of the Asparagales and Liliales is reviewed with references to their morphology, embryology, DNA analyses, and other aspects. Asparagaceae, Ruscaceae, Dracenaceae, and Nolinaceae may share a common ancestor with x = 10, and Tecophilaeaceae, Cyanastraceae, and Ixioliriaceae with x = 12. Tricyrtis (Uvulariaceae) is karyologically similar to Tulipa, Gagea (Liliaceae) and Smilax (Smilacaceae); Clintonia (Uvulariaceae) to Medeola (Liliaceae); Prosartes and Streptopus (Uvulariaceae) to Calochortus (Calochortaceae); Scoliopus (Trilliaceae) to the Medeola‐Clintonia group or Calochortus‐Prosartes‐Streptopus group. The original chromosome size of the Asparagales and Liliales may be estimated as small.