2018
DOI: 10.1016/j.cell.2018.01.027
|View full text |Cite
|
Sign up to set email alerts
|

Chromosome Translocation Inflates Bacillus Forespores and Impacts Cellular Morphology

Abstract: The means by which the physicochemical properties of different cellular components together determine bacterial cell shape remain poorly understood. Here, we investigate a programmed cell-shape change during Bacillus subtilis sporulation, when a rod-shaped vegetative cell is transformed to an ovoid spore. Asymmetric cell division generates a bigger mother cell and a smaller, hemispherical forespore. The septum traps the forespore chromosome, which is translocated to the forespore by SpoIIIE. Simultaneously, fo… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

5
84
1

Year Published

2018
2018
2024
2024

Publication Types

Select...
4
3
1

Relationship

2
6

Authors

Journals

citations
Cited by 51 publications
(90 citation statements)
references
References 67 publications
5
84
1
Order By: Relevance
“…In contrast with analyses of IID and IIP mutants in B. subtilis , no forespore bulging was observed in C. difficile ∆ IID or ∆ IIP mutants by TEM . Forespore bulging occurs when the forespore pushes into the mother cell through a small area of apparently weakened cell wall (Frandsen and Stragier, ; Eichenberger et al ., ; Gutierrez et al ., ) to balance the turgor pressure generated by chromosome packing into the small forespore (Lopez‐Garrido et al ., ). C. difficile ∆ IIQ sporulating cells, nevertheless, produced forespore bulges in 8% of ∆ IIQ sporulating cells analyzed, consistent with structured illumination microscopy (SIM) analyses of an independent ∆ IIQ mutant in broth‐grown cultures (Serrano et al ., ).…”
Section: Resultsmentioning
confidence: 97%
See 2 more Smart Citations
“…In contrast with analyses of IID and IIP mutants in B. subtilis , no forespore bulging was observed in C. difficile ∆ IID or ∆ IIP mutants by TEM . Forespore bulging occurs when the forespore pushes into the mother cell through a small area of apparently weakened cell wall (Frandsen and Stragier, ; Eichenberger et al ., ; Gutierrez et al ., ) to balance the turgor pressure generated by chromosome packing into the small forespore (Lopez‐Garrido et al ., ). C. difficile ∆ IIQ sporulating cells, nevertheless, produced forespore bulges in 8% of ∆ IIQ sporulating cells analyzed, consistent with structured illumination microscopy (SIM) analyses of an independent ∆ IIQ mutant in broth‐grown cultures (Serrano et al ., ).…”
Section: Resultsmentioning
confidence: 97%
“…), in contrast with the flat septa of B. subtilis ∆ IIDPM cells (Eichenberger et al ., ; Ramamurthi et al ., ). Again, it is unclear why these differences are observed, but an additional peptidoglycan remodeling system initiating engulfment in C. difficile might be at play or outward pressure from the forespore nucleoid (Lopez‐Garrido et al ., ) could cause bending of the C. difficile septum as discussed above .…”
Section: Discussionmentioning
confidence: 95%
See 1 more Smart Citation
“…Pressure could be measured in the mother cell and forespore by AFM indentation and fluorescence microscopy [28,29]. Indeed, packing of charged DNA in the small forespore may induce osmotic swelling in line with Region III [30]. High-throughput imaging could be used to test if some cells grow despite sporulation.…”
Section: Discussionmentioning
confidence: 99%
“…S7) we used semi-automated ImageJ plugin JFilament (Smith et al, n.d.). After extracting the coordinates of the micro-colony contours from phase-contrast images, colony area was calculated as the area of the corresponding polygon (Lopez-Garrido et al, 2018;Ojkic et al, 2016).…”
Section: Microscopy a = Ln10 Dtmentioning
confidence: 99%