During 1992, 83 specimens of North American Amphibia from Algonquin Park and Kingston, Ontario, Canada, and from El Paso County, Texas USA, comprising 14 species in seven families, were checked for opalinids in the cloaca. Protoopalina allisteri n. sp. is described from one Gastrophryne carolinensis (Microhylidae) from Arkansas and P. mitotica was found in one of four Scaphiopus multiplicatus (Pelobatidae) from Texas. The North American species of Protoopalina are reviewed. Zelleriella ovoidea (Metcalf, 1923) is proposed as a new combination for P. ovoidea.