Karyotypic analysis was carried out from ten most commonly occurring aphids, namely Aphis gossypii (2n=8), Aphis nasturtii (2n=8), Aulacorthum solani (2n= 10), Aulacorthum sp. (2n=12), Greenidea formosana (2n= 18), Macrosiphoniella sp. (2n=12), Macrosiphum rosae (2n=10), Myzus persicae (2n=12), Rhopalosiphum maidis (2n=8) and Toxoptera aurantii (2n=8), infesting different host plants from Kangra and Shimla regions of Himachal Pradesh, India.