Chronic cavitary pulmonary aspergillosis in an immunocompetent child

Davies, Adeyinka A.; Adegbite, Ireti A; Akintan, Patricia; Ibrahim, Usman O; Adekoya, Abiola Omobonike; Oladele, Rita O.

doi:10.1016/j.mmcr.2022.07.001

Cited by 1 publication

(1 citation statement)

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“…The proven diagnosis depends on histopathological biopsy, microscopic analysis of sterile material, culture of sterile material, blood culture, tissue nucleic acid diagnosis ( Donnelly et al., 2020 ). Due to absence of typical clinical presentation, the low vigilance of IPA in clinical practice, and the difficulty in obtaining qualified specimens, IPA is easily underdiagnosed and misdiagnosed ( Davies et al., 2022 ; Ekeng et al., 2022 ), resulting in patients missing treatment opportunities and even death ( Lowes et al., 2017 ). Therefore, it is urgent that novel detection methods are needed to meet the clinical requirements.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic efficacy of metagenomic next generation sequencing in bronchoalveolar lavage fluid for proven invasive pulmonary aspergillosis

Jia,

Liu,

et al. 2023

Front. Cell. Infect. Microbiol.

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ObjectiveTo assess the diagnostic efficacy of metagenomic next generation sequencing (mNGS) for proven invasive pulmonary aspergillosis (IPA).MethodsA total of 190 patients including 53 patients who had been diagnosed with proven IPA were retrospectively analyzed. Using the pathological results of tissue biopsy specimens as gold standard, we ploted the receiver operating characteristic (ROC) curve to determine the optimal cut-off value of mNGS species-specific read number (SSRN) of Aspergillus in bronchoalveolar lavage fluid (BALF)for IPA. Furthermore, we evaluated optimal cut-off value of mNGS SSRN in different populations.ResultsThe optimal cut-off value of Aspergillus mNGS SSRN in BALF for IPA diagnosis was 2.5 for the whole suspected IPA population, and 1 and 4.5 for immunocompromised and diabetic patients, respectively. The accuracy of mNGS was 80.5%, 73.7% and 85.3% for the whole population, immunocompromised and diabetic patients, respectively.ConclusionsThe mNGS in BALF has a high diagnostic efficacy for proven IPA, superioring to Aspergillus culture in sputum and BALF and GM test in blood and BALF. However, the cut-off value of SSRN should be adjusted when in different population.

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