Chronic chemogenetic manipulation of ventral pallidum targeted neurons in male rats fed an obesogenic diet

Doucette, Wilder; Smedley, Elizabeth B.; Ruiz-Jaquez, Metztli J.; Khokhar, Jibran Y.; Smith, Ken G.

doi:10.1016/j.brainres.2022.147886

Cited by 3 publications

(1 citation statement)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Building upon evidence that VP is altered by exposure to high-fat diet ( 26; 27 ), controls preference for fatty foods ( 48 ), and modulates weight gain from high-fat diet ( 49 ), we investigated how prolonged high-fat diet changes widespread gene expression in VP. We detected DEGs in all cell types when comparing gene expression after 5 w of high-fat diet to 5 w of matched diet and to 2 w of standard diet.…”

Section: Discussionmentioning

confidence: 99%

Single-cell sequencing of rodent ventral pallidum reveals diverse neuronal subtypes with non-canonical interregional continuity

Ottenheimer,

Simon,

Burke

et al. 2024

Preprint

View full text Add to dashboard Cite

The ventral pallidum (VP) was defined as a basal ganglia nucleus with dense input from ventral striatum. To further investigate a VP regional identity, we conducted a cross-species transcriptional characterization of VP cell types. We performed single nucleus RNA-sequencing of VP tissue from mice and rats and identified 16 VP neuronal subclasses with striking cross-species conservation. VP GABAergic neurons were surprisingly heterogeneous, consisting of 14 sub-classes from 3 developmental classes. Combining our sequencing data with a spatial atlas revealed that all VP subclasses extended beyond the traditional borders of VP. Integrating our VP data with prior sequencing data from striatal, hypothalamic, and extended amygdalar tissue confirmed that cell types are shared among these regions. Due to the role of VP in feeding behavior, we also assessed the transcriptional impact of high-fat diet consumption, which induced altered expression of genes involved in oxidative phosphorylation and inhibitory signaling. Overall, our results demonstrate that VP is not a transcriptionally discrete nucleus; rather, VP contains cell types with diverse expression patterns that overlap with regions beyond the basal ganglia.

show abstract

Section: Discussionmentioning

confidence: 99%

Single-cell sequencing of rodent ventral pallidum reveals diverse neuronal subtypes with non-canonical interregional continuity

Ottenheimer,

Simon,

Burke

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Differential role of GABAergic and cholinergic ventral pallidal neurons in behavioral despair, conditioned fear memory and active coping

Akmese

Sevinc

Halim

et al. 2023

Progress in Neuro-Psychopharmacology and Biological Psychiatry

View full text Add to dashboard Cite

Ventral Pallidum Neurons Are Necessary to Generalize and Express Fear-Related Responding in a Minimal Threat Setting

Russell,

McDannald

2024

eNeuro

View full text Add to dashboard Cite

Fear generalization is a hallmark of anxiety disorders. Experimentally, fear generalization can be difficult to dissociate from its counterpart, fear discrimination. Here we use minimal threat learning procedures to reveal such a dissociation. We show that in Long Evans rats, an auditory threat cue predicting foot shock on 10% of trials produces a discriminated fear response that does not generalize to a neutral auditory cue. Even slightly higher foot shock probabilities (30% and 20%) produce fear generalization. AAV-mediated, caspase-3 deletion of ventral pallidum neurons abolishes fear generalization and reduces threat cue responding during extinction. The ventral pallidum’s contribution to fear generalization and extinction threat responding does not depend on inputs from the nucleus accumbens. The results demonstrate a minimal threat learning approach to dissociate fear discrimination from fear generalization, and a novel role for the ventral pallidum in generalizing and expressing fear.Significance StatementIn the laboratory, healthy mice, rats, and people generalize fear responding to a neutral cue before showing fear discrimination. However, in the real world, fear generalization is not nearly as ubiquitous in healthy individuals. Here we show that in rats, minimal threat learning procedures manipulating foot shock probability identify a boundary at which fear discrimination proceeds in the absence of fear generalization. We exploit this boundary to reveal a novel and essential role for the ventral pallidum in fear generalization.

show abstract

Chronic chemogenetic manipulation of ventral pallidum targeted neurons in male rats fed an obesogenic diet

Cited by 3 publications

References 59 publications

Single-cell sequencing of rodent ventral pallidum reveals diverse neuronal subtypes with non-canonical interregional continuity

Single-cell sequencing of rodent ventral pallidum reveals diverse neuronal subtypes with non-canonical interregional continuity

Differential role of GABAergic and cholinergic ventral pallidal neurons in behavioral despair, conditioned fear memory and active coping

Ventral Pallidum Neurons Are Necessary to Generalize and Express Fear-Related Responding in a Minimal Threat Setting

Contact Info

Product

Resources

About