Chronic Consumption of Sweeteners Increases Carbonylated Protein Production in Lymphocytes from Mouse Lymphoid Organs

Abstract

Background: The prevalence of overweight, obesity and diabetes mellitus has increased in Mexico, therefore, sucralose and stevia are being used as alternative non-caloric sweeteners to reduce energy intake. Moreover, poorly balanced diets can lead to the formation of carbonyl groups, a marker used to determine oxidative damage to proteins. Increased presence of carbonylated proteins in CD1 mice chronically consuming sweeteners, may point them as causing oxidative damage. Aims: To determine whether the continued use of natural and artificial sweeteners increases the presence of carbonylated proteins in lymphocytes of lymphoid tissues in CD1 male mice. Methods: The present study was conducted with 72 CD1 newly weaned (21-day old) male mice, fed with standard lab diet and water ad libitum; mice were hosted in cages in groups of 4 under controlled temperature conditions (19-21°C), and light/dark cycles of 12/12 h. Weight and food intake was quantified weekly. Three groups of mice were randomly conformed: a) Baseline (21-day

Effect of Chronic Commercial Sweeteners Consumption in Lymphocytes of Peyer’s Patches

“…Also, because they are not obese strains such as db/db and ob/ob mice. This allows us to evaluate the effect of diets and different nutrients in a healthy animal model [45,46,47,48]. The objective of using this strain was to work with healthy rodent models, to know the effect of sweeteners on healthy subjects before the disease is established.…”