Chronic Copper Toxicity in Lambs: A Survey of Blood Constituent Responses

Buckley, W. T.; Tait, R. M.

doi:10.4141/cjas81-074

Cited by 13 publications

(4 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some individual animals have activities near the normal values, but the means for the group rise with increasing hepatic Cu-level. For the activities of GOT this finding agrees with those of others (4,9), but it can be concluded that the activities of SDH and y-GT are also of value in establishing a high Cu-status.…”

Section: Discussionsupporting

confidence: 91%

See 1 more Smart Citation

Levels of enzyme activities in blood serum as indicators of a high copper status in sheep flocks

Schee¹,

Schotman²,

Berg³

1983

Zentralblatt für Veterinärmedizin Reihe A

View full text Add to dashboard Cite

Summary The relationship between the hepatic Cu‐level and certain biochemical constituents in the blood of lambs was studied. The activities of the enzymes glutamic oxaloacetic transaminase (E.C. 2.6.1.1.), sorbitol dehydrogenase (E.C. 1.1.1.14.) and to a lesser extent γ‐glutamyl transferase (E.C. 2.3.2.2.) can be considered as indicators of a high copper status of a flock. When the copper status is very high (< 1500 μg Cu per g DM in the liver) the activity of lactate dehydrogenase (E.C. 1.1.1.27.) is strongly enhanced. For individual animals, the estimation of these enzymes is of little diagnostic value in predicting the Cu‐status. The influence of hepatic Cu‐levels lower than 600 μg per g DM on any of the parameters determined in the blood was slight. Zusammenfassung Enzymaktivitäten im Blutserum als Indikatoren für einen hohen Kupferstatus in Schafherden Die Beziehungen zwischen dem Kupfergehalt der Leber und gewissen biochemischen Parametern im Blut der Lämmer wurde untersucht. Die Aktivitäten folgender Enzyme können als Indikatoren für einen hohen Kupferstatus einer Herde betrachtet werden: Glutamat‐Oxalacetat‐Transaminase (E.C. 2.6.1.1.), Sorbit‐Dehydrogenase (E.C. 1.1.1.14.) und in einem geringeren Ausmaß γ‐Glutamyl‐Transferase (E.C. 2.3.2.2.). Bei sehr hohem Kupferstatus (< 1500 μg Cu pro g Trockenmasse in der Leber) ist auch die Aktivität der Laktat‐Dehydrogenase (E.C. 1.1.1.27.) stark erhöht. Bei individuellen Tieren dagegen ist die Bestimmung der Enzymaktivitäten von geringer diagnostischer Aussagekraft bezüglich des Kupferstatus. Bei Kupfergehalten in der Leber von weniger als 600 μg pro g TM war der Einfluß auf die untersuchten Parameter nur gering. Résumé Activités enzymatiques dans le sérum sanguin comme indicateurs d'un statut élevé du cuivre dans des troupeaux de moutons On a examiné le rapport entre le taux du cuivre dans le foie et certains paramètres biochimiques dans le sang d'agneaux. Les activités enzymatiques suivantes peuvent être considérées comme indicateurs pour un statut élevé en cuivre d'un troupeau: glutamate‐oxalacétate‐transaminase (E.C. 2.6.1.1.). sorbit‐déhydrogénase (E.C. 1.1.1.14.) et dans une moindre mesure γ‐glutamyl‐transférase (E.C. 2.3.2.2.). L'activité de lactate‐déhydro‐génase (E.C. 1.1.1.27.) est fortement augmentée avec un statut très élevé du cuivre (> 1500 μg Cu pro g substance sèche dans le foie). La détermination des activités enzymatiques est d'une moindre valeur pour le diagnostic concernant le statut du cuivre chez des animaux pris individuellement. L'influence sur les paramètres recherchés fut faible avec des taux de cuivre dans le foie inférieurs à 600 μg pro g substance sèche. Resumen Los niveles de actividades enzimáticas en el suero sanguíneo coma indicadores del estado elevado de cobre en rebaños de ovinos Se examinaron las relaciones entre el contenido de cobre en el hígado y ciertos parámetras bioquímicos en la sangre de los corderas. Las actividades de las enzimas siguientes se pueden considerar como indicadoras de un estado elevado de cobre: glutamat...

show abstract

Section: Discussionsupporting

confidence: 91%

“…This fact may be of value to assess that the animals are in the terminal phase of the disease. The apparently conflicting conclusions about L D H in the literature (4,9,10) of Cu-intoxicition may be due to differences in the severity of the Cuaccumulation. .…”

Section: Discussionmentioning

confidence: 98%

Levels of enzyme activities in blood serum as indicators of a high copper status in sheep flocks

Schee¹,

Schotman²,

Berg³

1983

Zentralblatt für Veterinärmedizin Reihe A

View full text Add to dashboard Cite

show abstract

“…Buckley and Tait (1981) also found that growth rate was unaffected during the period of liver Cu accumulation. The period of Cu accumulation before growth rate was affected was 12 weeks.…”

Section: Discussionmentioning

confidence: 83%

Comparative study of chronic copper poisoning in lambs and young goats

1990

View full text Add to dashboard Cite

Eighteen lambs (L) and 18 goats (G), 12 weeks of age, were allocated in equal numbers to three diets. The basal (B) diet had no added copper (Cu) while the other two had 30 or 60 mg added Cu per kg dry matter (DM) as CuSO 4 .5H 2 O. Lambs and goats were housed and fed individually. After 67 days, deaths from Cu toxicity occurred only in lambs from groups L-30 (basal diet + 30 mg Cu per kg DM) and L-60 (basal diet + 60 mg Cu per kg DM). The remaining lambs and all of the goats were slaughtered at 91 and 137 days respectively.A decreased food intake and loss of weight were observed in some lambs towards the end of the experiment which appeared to be associated with an approaching haemolytic crisis. The additional dietary Cu had a positive effect on food conversion efficiency only in the early stages of the experiment in both lambs and goats. None of the goats died or lost weight.Plasma Cu concentrations and serum glutamate oxalacetate transaminase and creatine kinase activities were elevated in lambs of groups L-30 and L-60 after 2 months, while in goats the concentrations were in normal ranges during the whole experimental period.Additional dietary Cu increased significantly the Cu concentrations of liver, kidney, muscle, spleen, heart, brain, hair and faeces in both lambs and goats. Additional Cu decreased the fresh and dry liver weights of lambs and the liver to live weight ratio whereas the corresponding values for kidneys were increased. The differences between groups of goats in fresh or dry liver and kidney weights were not significant, though the fresh and dry liver weights tended to be higher in groups G-30 and G-60 compared with those in the group G-B.The amount of Cu stored in the livers of the lambs was six to nine times higher than that of the goats. Differences in Cu storage between lambs and goats may be related to species differences in Cu utilization and hence resistance to Cu toxicity.

show abstract

“…Liver Suttle (1974a,b), Dalgarno and Mills (1975), Suttle and Price (1976), Buckley and Tait (1981) and Woolliams et al (1985) who also found that Cu as copper su$hate was readily absorbed by sheep. In contrast, Ivan and Veira (1985), in a study similar to the present one, showed that copper sulphate supplementation of 6 mg kg-'DM to a corn in AAT activitv (Kelleher and Ivan 1987).…”

Section: Discussionmentioning

confidence: 99%