Chronic corticosterone disrupts the circadian rhythm of CRH expression and m6A RNA methylation in the chicken hypothalamus

Yang, Yang; Han, Wanwan; Zhang, Aijia; Zhao, Meifang; Cong, Wei; Jia, Yimin; Wang, Deyun; Zhao, Ruqian

doi:10.1186/s40104-022-00677-4

Cited by 11 publications

(4 citation statements)

References 51 publications

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“…Hao et al showed that METTL3-mediated m6A modification plays an important role in biological processes such as mouse embryo development ( 8 ). Yang et al found that RNA m6A modification is involved in the regulation of circadian rhythms in the chicken hypothalamus under both basal and chronic stress conditions ( 9 ). m6A modification also has many functions in embryonic stem cell differentiation, development of the nervous and hematopoietic systems, myogenesis, zygote formation, and embryonic development ( 10 ).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome-wide study revealed m6A and miRNA regulation of embryonic breast muscle development in Wenchang chickens

Jiang

Chen³

et al. 2022

Front. Vet. Sci.

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N6-Methyladenosine (m6A) modification has been shown to play important role in skeletal muscle development. Wenchang chickens are commonly used as a high-quality animal model in researching meat quality. However, there have been no previous reports regarding the profile of m6A and its function in the embryonic breast muscle development of Wenchang chickens. In this paper, we identified different developmental stages of breast muscle in Wenchang chickens and performed m6A sequencing and miRNA sequencing in the breast muscle of embryos. Embryo breast muscles were weighed and stained with hematoxylin–eosin after hatching. We found that myofibers grew fast on the 10th day after hatching (E10) and seldom proliferated beyond the 19th day after hatching (E19). A total of 6,774 differentially methylated genes (DMGs) were identified between E10 and E19. For RNA-seq data, we found 5,586 differentially expressed genes (DEGs). After overlapping DEGs and DMGs, we recorded 651 shared genes (DEMGs). Subsequently, we performed miRNA-seq analysis and obtained 495 differentially expressed miRNAs (DEMs). Then, we overlapped DEMGs and the target genes of DEMs and obtained 72 overlapped genes (called miRNA-m6A-genes in this study). GO and KEGG results showed DEMGs enriched in many muscle development-related pathways. Furthermore, we chose WNT7B, a key regulator of skeletal muscle development, to perform IGV visualization analysis and found that the m6A levels on the WNT7B gene between E10 and E19 were significantly different. In conclusion, we found that miRNAs, in conjunction with m6A modification, played a key role in the embryonic breast muscle development of Wenchang chickens. The results of this paper offer a theoretical basis for the study of m6A function in muscle development and fat deposition of Wenchang chickens.

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Section: Introductionmentioning

confidence: 99%

Transcriptome-wide study revealed m6A and miRNA regulation of embryonic breast muscle development in Wenchang chickens

Jiang

Chen³

et al. 2022

Front. Vet. Sci.

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“…One possible explanation is that circadian patterns of hormone activities or concentrations are controlled by the duration of photophase, as has been shown in other investigations [ 56 ]. The secretion of CORT is regulated by the hypothalamic–pituitary–adrenal axis (HPA-axis) via the adrenocorticotrophic hormone (ACTH) and local adrenocortical catecholamine secretion [ 57 , 58 ]. Considering the influence of light on CORT production, an increased photoperiod under stable conditions is able to stimulate gonadotropine-releasing hormone (GnRH) secretion, which, in turn, enhances steroid production [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Circadian Corticosterone Profile in Laying Hens (Gallus gallus domesticus)

Hillebrecht,

Korbel,

Rinder

et al. 2024

Animals

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Measurement of blood corticosterone concentrations has been established as an indicator for assessment of acute distress. Therefore, knowledge on physiological fluctuations is required, but previous studies allow little conclusion on daily fluctuations in domestic chickens (Gallus gallus domesticus). To verify the presence of a circadian corticosterone rhythm in socialized chickens, blood samples were taken at four-hour intervals from 12 laying hens kept in groups of four over three days, each. Prior to experiments, hens were adapted to repeated handling for stress reduction. Corticosterone concentration was determined using radioimmunoassay. Blood sampling time and duration were recorded, and audio and video recordings were analyzed to assess the impact of behavior on corticosterone concentrations. Despite individual fluctuations, most hens showed a circadian course with two peaks per day. Statistics revealed a significant peak during the day (between 12:00 p.m. and 04:00 p.m.) and a tendency for a second peak at night (12:00 a.m.). The daily corticosterone peak was not explained by daytime social stress and needs to be seen as an endophenotype. The role of nightly corticosterone production has to be investigated in further studies. There might be a relation between corticosterone and reproduction since the only hen not showing peaks was not laying eggs.

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“…So, the difference of central circadian system of chickens and mammals could be the major cause for this issue. Additionally, monochromatic light (e.g., green and red light) and chronic corticosterone had a significant impact on the expression pattern of circadian genes in the hypothalamus of chickens [6,32].…”

Section: Discussionmentioning

confidence: 99%

Novel Insights into the Circadian Rhythms Based on Long Noncoding and Circular RNA Profiling

Tan,

Zhang,

Dong

et al. 2024

IJMS

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Circadian rhythm disorders pose major risks to human health and animal production activity, and the hypothalamus is the center of circadian rhythm regulation. However, the epigenetic regulation of circadian rhythm based on farm animal models has been poorly investigated. We collected chicken hypothalamus samples at seven time points in one light/dark cycle and performed long noncoding RNA (lncRNA), circular RNA (circRNA), and mRNA sequencing to detect biomarkers associated with circadian rhythm. We enhanced the comprehensive expression profiling of ncRNAs and mRNAs in the hypothalamus and found two gene sets (circadian rhythm and retinal metabolism) associated with the light/dark cycle. Noncoding RNA networks with circadian expression patterns were identified by differential expression and circadian analysis was provided that included 38 lncRNAs, 15 circRNAs, and 200 candidate genes. Three lncRNAs (ENSGALT00000098661, ENSGALT00000100816, and MSTRG.16980.1) and one circRNA (novel_circ_010168) in the ncRNA–mRNA regulatory network were identified as key molecules influencing circadian rhythm by regulating AOX1 in retinal metabolism. These ncRNAs were predicted to be related to pernicious anemia, gonadal, eye disease and other disorders in humans. Together, the findings of this study provide insights into the epigenetic mechanisms of circadian rhythm and reveal AOX1 as a promising target of circadian rhythm regulation.

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Chronic corticosterone disrupts the circadian rhythm of CRH expression and m6A RNA methylation in the chicken hypothalamus

Cited by 11 publications

References 51 publications

Transcriptome-wide study revealed m6A and miRNA regulation of embryonic breast muscle development in Wenchang chickens

Transcriptome-wide study revealed m6A and miRNA regulation of embryonic breast muscle development in Wenchang chickens

Circadian Corticosterone Profile in Laying Hens (Gallus gallus domesticus)

Novel Insights into the Circadian Rhythms Based on Long Noncoding and Circular RNA Profiling

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