Chronic cuffing of cervical vagus nerve inhibits efferent fiber integrity in rat model

Somann, Jesse P.; Albors, Gabriel O.; Neihouser, Kaitlyn V.; Lu, Kun‐Han; Li, Zhong‐Ming; Ward, Matthew; Durkes, Abigail C.; Robinson, J. Paul; Powley, Terry L.; Irazoqui, Pedro P.

doi:10.1088/1741-2552/aaa039

Cited by 34 publications

(36 citation statements)

References 72 publications

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“…Vagus nerve recordings have been conducted under anesthesia for both acute (WOODBURY & WOODBURY 1991;Caravaca et al 2017;Dias Da Silva et al 2002;Krolczyk et al 2008) and chronic models (Caravaca et al 2017;Sauter et al 1983). Others have chronically recorded from the peripheral sciatic nerve in awake rat models (Michoud et al 2017;Srinivasan et al 2015;Gore et al 2015;Srinivasan et al 2016;Musick et al 2015;Vasudevan et al 2017) and from the vagus nerve in anesthetized rat models (Khodaparast et al 2014;Khodaparast et al 2016;Somann et al 2018), but these devices have not been translated to the mouse vagus nerve model. Here we demonstrated that this microwire based approach was effective in obtaining chronic neural recordings and identifiable CAPs from the cervical vagus nerve in mice.…”

Section: Discussionmentioning

confidence: 99%

“…) of the vagus nerve. Preclinical studies for chronic vagus nerve electrodes have been successfully conducted in large animal models (Zhang et al 2009;Ardell et al 2017;Hamann et al 2013;Metcalfe et al 2018;Valdés-Cruz et al 2002) and in rats (Somann et al 2017;McCallum et al 2017;Grimonprez et al 2015;Li et al 2004;Khodaparast et al 2016). Thus far, no studies for chronic mouse vagus nerve interfacing have been reported.…”

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confidence: 99%

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A wrappable microwire electrode for awake, chronic interfacing with small diameter autonomic peripheral nerves

Goldman

et al. 2018

Preprint

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Bioelectronic medicine requires the ability to monitor and modulate nerve activity in awake patients over time. The vagus nerve is a promising stimulation target, and preclinical models often use mice. However, an awake, chronic mouse vagus nerve interface has yet to be demonstrated. Here, we developed a functional wrappable microwire electrode to chronically interface with the small diameter mouse cervical vagus nerve (~100 μ m). In an acute setting, the wrappable microwire had similar recording performance to commercially available electrodes. A chronic, awake mouse model was then developed to record spontaneous compound action potentials (CAPs). Viable signal-to-noise ratios (SNRs) were obtained from the wrappable microwires between 30 and 60 days (n = 8). Weekly impedance measurements showed no correlation between SNR or time. The wrappable microwires successfully interfaced with small diameter nerves and has been validated in a chronic, awake preclinical model, which can better facilitate clinical translation for bioelectronic medicine.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

A wrappable microwire electrode for awake, chronic interfacing with small diameter autonomic peripheral nerves

Goldman

et al. 2018

Preprint

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“…However, conventional chronic nerve interfaces have geometries and mechanical properties that remain orders of magnitude divergent from those of the implant targets, increasing the risk of insult to host tissues, incidental damage to the nerve, and diminished efficacy due to poor compliance with the nerve (Grill et al, 2009;Minev et al, 2015;Salatino et al, 2017;Somann et al, 2017;Tyler and Durand, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies suggest that some approaches to PNS interfacing are associated with significant compromise of nerve health at both acute and chronic timepoints (Rydevik et al, 1981;Somann et al, 2017;Tyler and Durand, 2003), casting doubt on prior findings establishing the basic physiology of the periphery and the feasibility of bioelectronic therapeutic and restorative technologies. Thus, one of the principal design constraints was to develop an interface that minimized surgical complexity such that insult to the targeted nerve and the surrounding tissues would be minimal.…”

Section: Uncompromised Nerve Function Following Implantmentioning

confidence: 99%

“…A variety of peripheral neural interfacing approaches have been previously reported -including extra-neural (Naples and Sweeney, 1988;Tan et al, 2014;Tyler and Durand, 2002), penetrating (Badia et al, 2011;Boretius et al, 2010;Christensen et al, 2014;Lago et al, 2007;Wark et al, 2013Wark et al, , 2014, and regenerative electrodes (Lacour et al, 2008(Lacour et al, , 2009) -each with unique sets of advantages and disadvantages for specific applications. For example, traditional extra-neural cuff electrodes interface with intact whole nerves, but have limited recording capability and are a challenge to size properly for fine nerve targets (Loeb and Peck, 1996;Navarro et al, 2005;Somann et al, 2017). Intrafascicular penetrating microelectrodes can realize significant improvements in recording and stimulating specificity, but risk substantial insertion trauma and existing platforms are too large for chronic use in many fine nerves.…”

Section: Introductionmentioning

confidence: 99%

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Printable microscale interfaces for long-term peripheral nerve mapping and precision control

Otchy

Michas

Lee

et al. 2019

Preprint

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The nascent field of bioelectronic medicine seeks to decode and modulate peripheral nervous system signals to obtain therapeutic control of targeted end organs and effectors. Current approaches rely heavily on electrodebased devices, but size scalability, material and microfabrication challenges, limited surgical accessibility, and the biomechanically dynamic implantation environment are significant impediments to developing and deploying advanced peripheral interfacing technologies. Here, we present a microscale implantable device -the nanoclipfor chronic interfacing with fine peripheral nerves in small animal models that begins to meet these constraints.We demonstrate the capability to make stable, high-resolution recordings of behaviorally-linked nerve activity over multi-week timescales. In addition, we show that multi-channel, current-steering-based stimulation can achieve a high degree of functionally-relevant modulatory specificity within the small scale of the device. These results highlight the potential of new microscale design and fabrication techniques for the realization of viable implantable devices for long-term peripheral interfacing.

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Characterization of a cell bridge variant connecting the nodose and superior cervical ganglia in the mouse: Prevalence, anatomical features, and practical implications

Bookout

Gautron

2020

J of Comparative Neurology

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While autonomic ganglia have been extensively studied in rats instead of mice, there is renewed interest in the anatomy of the mouse autonomic nervous system. This study examined the prevalence and anatomical features of a cell bridge linking two autonomic ganglia of the neck, namely, the nodose ganglion (NG) and the superior cervical ganglion (SCG) in a cohort of C57BL/6J mice. We identified a cell bridge between the NG and the cranial pole of the SCG. This cell bridge was tubular shaped with an average length and width of 700 and 240 μm, respectively. The cell bridge was frequently unilateral and significantly more prevalent in the ganglionic masses from males (38%) than females (21%). On each of its extremities, it contained a mixed of vagal afferents and postganglionic sympathetic neurons. The two populations of neurons abruptly replaced each other in the middle of the cell bridge. We examined the mRNA expression for selected autonomic markers in samples of the NG with or without cell bridge. Our results indicated that the cell bridge was enriched in both markers of postganglionic sympathetic and vagal afferents neurons. Lastly, using FluoroGold microinjection into the NG, we found that the existence of a cell bridge may occasionally lead to the inadvertent contamination of the SCG. In summary, this study describes the anatomy of a cell bridge variant consisting of the fusion of the mouse NG and SCG. The practical implications of our observations are discussed with respect to studies of the mouse vagal afferents, an area of research of increasing popularity.

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Chronic cuffing of cervical vagus nerve inhibits efferent fiber integrity in rat model

Cited by 34 publications

References 72 publications

A wrappable microwire electrode for awake, chronic interfacing with small diameter autonomic peripheral nerves

A wrappable microwire electrode for awake, chronic interfacing with small diameter autonomic peripheral nerves

Printable microscale interfaces for long-term peripheral nerve mapping and precision control

Characterization of a cell bridge variant connecting the nodose and superior cervical ganglia in the mouse: Prevalence, anatomical features, and practical implications

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