Chronic cyanide exposure

Downey, Jason D.; Basi, Kelly A.; DeFreytas, Margaret R.; Rockwood, Gary A.

doi:10.1002/9781118628966.ch2

Cited by 1 publication

(1 citation statement)

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“…The accumulation of cyanide in the environment poses risks to ecosystems and human health due to its ability to inhibit essential metalloenzymes [19,20]. Conventional physicochemical methods for cyanide removal have limitations like high costs, secondary pollution, and the inability to completely destroy cyanide [21].…”

Section: Introductionmentioning

confidence: 99%

Cyanide Bioremediation by Bacillus subtilis under Alkaline Conditions

Cáceda Quiroz,

Fora Quispe,

Carpio Mamani

et al. 2023

Water

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Cyanide (CN) is a toxic environmental pollutant generated by various industrial activities, necessitating the application of bioremediation techniques for its degradation. Biodegradation is a more cost-effective and environmentally friendly technique with high efficiency in CN removal. This study isolated cyanide-degrading bacteria from Tutupaca mining site soil from Tacna, Peru. Bacillus subtilis strain TT10s was selected for its exceptional capacity to rapidly and completely eliminate cyanide under alkaline conditions (pH 10.5), removing 1000 ppm cyanide within 48 h. A kinetic analysis revealed that the biodegradation follows second-order rate kinetics (k2 = 0.08649 mg/(mg·h), R2 = 0.96622), consistent with the literature attribution of the rate-limiting step to the inducible cyanide dihydratase enzyme, which converts cyanide into ammonia and formate via the Michaelis–Menten model. Fourier-transform infrared spectroscopy (FTIR) spectral analysis further corroborated this enzymatic mechanism, showing the disappearance of CN peaks coupled with the emergence of ammonia (NH) and formate (C=O) peaks. Quantitative kinetic modelling integrated with FTIR profiles and degradation curves implicates cyanide dihydratase as the key rate-controlling enzyme in alkaline cyanide biodegradation without the need for an extra carbon source, generating interest for future bioremediation applications in highly contaminated environments.

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Section: Introductionmentioning

confidence: 99%