Chronic effects induced by ibuprofen on the freshwater bivalve Dreissena polymorpha

Parolini, Marco; Binelli, Andrea; Provini, A.

doi:10.1016/j.ecoenv.2011.04.025

Cited by 138 publications

(62 citation statements)

References 57 publications

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“…The coupled action of CYP-enzymes and GST normally produce, directly or indirectly, free radical intermediates that could enhance the production of ROS. Previous studies on zebra mussels exposed to environmentally relevant concentrations of PCM and IBU have shown notable increases of GST activity, suggesting the possible formation of reactive metabolites, which have been assumed as the main reason for the onset of oxidative stress (Parolini et al 2010(Parolini et al , 2011b. The data presented here agree with this hypothesis, as GST was significantly (P \ 0.01) induced by the mixture of NSAIDs, particularly after 96 h of exposure to the High condition.…”

Section: Discussionsupporting

confidence: 94%

“…Previous studies have investigated the sub-lethal effects induced by NSAIDs on the zebra mussel, Dreissena polymorpha, and have emphasised that environmentally relevant concentrations of DCF (Parolini et al 2011a) induce low cellular and negligible genetic damage, while IBU (Parolini et al 2011b) and PCM (Parolini et al 2010) have low to moderate cyto-genotoxic effects on this bivalve species. However, mussels are commonly exposed in the real environment to mixture of drugs, with toxicities that are not accurately measured by analysing the effects obtained for single compounds (Flaherty and Dodson 2005) because their interactions could result in unexpected toxicological behaviours.…”

Section: Discussionmentioning

confidence: 99%

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Sub-lethal effects induced by a mixture of three non-steroidal anti-inflammatory drugs (NSAIDs) on the freshwater bivalve Dreissena polymorpha

Parolini

Binelli

2011

Ecotoxicology

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Non-steroidal anti-inflammatory drugs (NSAIDs) are the sixth top-selling drugs worldwide and are commonly found in freshwater ecosystems in the high ng/l to low μg/l range. Recent studies have investigated both the acute and the chronic toxicity of single NSAIDs on different biological models, but these studies have completely neglected the fact that, in the environment, non-target organisms are exposed to mixtures of drugs that have unforeseeable toxicological behavior. This work investigated the sub-lethal effects induced by a mixture of three common NSAIDs, namely, diclofenac, ibuprofen and paracetamol, on the freshwater bivalve, the zebra mussel (Dreissena polymorpha). The mussels were exposed to three different environmental concentrations of the mixture (Low, Mid and High). A multi-biomarker approach was used to highlight cyto-genotoxic effects and the imbalance of the oxidative status of the treated specimens. The Neutral Red Retention Assay (NRRA) was used as a biomarker of cytotoxicity, whereas the activities of catalase, superoxide dismutase, glutathione peroxidase and glutathione S-transferase were measured to assess the role played by the oxidative stress enzymes. In addition, the single cell gel electrophoresis assay, the DNA Diffusion assay and the micronucleus test were used to investigate possible genotoxic effects. According to our NRRA results, each treatment was able to induce a significant cellular stress in bivalves, probably due to the raise of oxidative stress, as indicated by the alteration of enzyme activities measured in treated specimens. Moreover, the mixture induced significant enhancements of DNA fragmentation, which preluded fixed genetic damage, as highlighted by the increase of both apoptotic and micronucleated cells.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Sub-lethal effects induced by a mixture of three non-steroidal anti-inflammatory drugs (NSAIDs) on the freshwater bivalve Dreissena polymorpha

Parolini

Binelli

2011

Ecotoxicology

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“…IBP is a non-steroidal anti-inflammatory drug (NSAIDs) widely used in the treatment of pain, dysmenorrhea, inflammation and fever (Parolini et al, 2011). IBP is known to inhibit CYP450 enzymes, which was demonstrated in the present study.…”

Section: Metabolism Of Pharmaceutical Products In Polychaetes H Divementioning

confidence: 80%

“…Pharmaceutical compounds have been recently identified as potentially toxic for aquatic organisms (Quinn et al, 2008a(Quinn et al, , 2008bMartín-Díaz et al, 2009;Eades and Waring, 2010;Oviedo-Gómez et al, 2010;Parolini et al, 2011;Aguirre-Martínez et al, 2013a, 2013b. The present paper focused on compounds with different chemical properties and modes of action (MOA), representative of a range of pharmaceutical classes: carbamazepine (anti-epileptic and neuroactive drug, CBZ), ibuprofen (non-steroidal anti-inflammatory drugNSAIDs, IBP), fluoxetine (selective serotonin reuptake inhibitorSSRIs, anti-depressive drug, FX), 17α-ethynylestradiol (oral female contraceptive, steroid drug, EE2) and propranolol (β-blocker drug, PRO).…”

Section: Introductionmentioning

confidence: 99%

Bioavailability, oxidative stress, neurotoxicity and genotoxicity of pharmaceuticals bound to marine sediments. The use of the polychaete Hediste diversicolor as bioindicator species

Maranho

Baena-Nogueras

Lara-Martín

et al. 2014

Environmental Research

114

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“…The scope of growth of this organism was influenced at lower concentrations when exposed to 1 mg/L for 2 weeks [52]. The effects of ibuprofen at 0.2-8 μg/L were addressed to the bivalve D. polymorpha exposed for 96 h, which demonstrated a slight cytogenotoxic effect on the mussel hemocytes at the lowest concentration tested, whereas higher concentrations tested were able to significantly increase both genetic and cellular damage [100]. In addition, ibuprofen was suggested to have a considerable effect on the activities of antioxidant and detoxifying enzymes due to the notable oxidative status imbalances of the exposed specimens.…”

Section: Nonsteroidal Anti-inflammatory Drugs: the Example Of Diclofementioning

confidence: 99%

Bioassays Currently Available for Evaluating the Biological Potency of Pharmaceuticals in Treated Wastewater

Vasquez

Michael²,

Kümmerer

et al. 2015

The Handbook of Environmental Chemistry

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Water deprivation with regard to quantity and quality is one of the most important environmental problems of the century. The increasing demand of water resources puts pressure on the utilization of alternative sources such as treated wastewater. In the context of "reduce, reuse, and recycle," the inclusion of treated wastewater in the water cycle seems a promising practice for water management. The lack of general acceptance of stakeholders and public, however, still hinders the widespread application of wastewater reuse. A reason for this is, among others, the presence of contaminants of emerging concern in treated wastewater. This has led to an increased concern about direct and indirect effects to the environment and possible implications to human health. The development and application of bioassays able to identify and quantify the biological potency of treated wastewater is an ongoing research effort, especially when taking into consideration that a plethora of contaminants exist and interact in this complex matrix. This chapter summarizes available literature regarding the sensitivity of currently applied bioassays for assessing biological effects of treated wastewater and their correlation with chemical analysis. The focus is on pharmaceuticals since they represent one of the major groups of contaminants of emerging concern with many unanswered questions currently in place.

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Chronic effects induced by ibuprofen on the freshwater bivalve Dreissena polymorpha

Cited by 138 publications

References 57 publications

Sub-lethal effects induced by a mixture of three non-steroidal anti-inflammatory drugs (NSAIDs) on the freshwater bivalve Dreissena polymorpha

Sub-lethal effects induced by a mixture of three non-steroidal anti-inflammatory drugs (NSAIDs) on the freshwater bivalve Dreissena polymorpha

Bioavailability, oxidative stress, neurotoxicity and genotoxicity of pharmaceuticals bound to marine sediments. The use of the polychaete Hediste diversicolor as bioindicator species

Bioassays Currently Available for Evaluating the Biological Potency of Pharmaceuticals in Treated Wastewater

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