Chronic Experimental Allergic Encephalomyelitis Induced in Rabbits with Bovine White Matter Proteolipid Apoprotein

Williams, Rosemarie M.; Lees, Marjorie B.; Cambi, Franca; Macklin, Wendy B.

doi:10.1097/00005072-198209000-00004

Cited by 66 publications

(15 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our finding that nerve conduction abnormalities are restricted to the CNS in PLPEAE is consistent with the results of histological studies showing restriction of inflammation and demyelination to the CNS in PLP-EAE (Yamamura et al, 1986;Chalk et al, 1994). In other species, such as the rabbit (Williams et al, 1982;Cambi et al, 1983), guineapig (Yoshimura et al, 1985) and mouse (Satoh et al, 1987;Trotter et al, 1987;Tuohy et al, 1988;van der Veen et al, 1989) limited histological studies of the PNS have been normal in PLP-EAE. The electrophysiological and histological findings are also consistent with biochemical studies showing restriction of PLP to the CNS (Lees and Macklin, 1988).…”

Section: Discussionsupporting

confidence: 89%

Conduction abnormalities are restricted to the central nervous system in experimental autoimmune encephalomyelitis induced by inoculation with proteolipid protein but not with myelin basic protein

Chalk

McCombe

Pender

1994

Brain

View full text Add to dashboard Cite

Experimental autoimmune encephalomyelitis (EAE) is an inflammatory demyelinating disease of the central nervous system (CNS) and can be induced by inoculation of animals with homogenized CNS tissue or highly purified myelin proteins such as myelin basic protein (MBP) or proteolipid protein (PLP). It is widely studied as a possible animal model of multiple sclerosis. We performed the present neurophysiological study to define the location of nerve conduction abnormalities in EAE induced by immunization with PLP (PLP-EAE) and in EAE induced by immunization with MBP (MBP-EAE) in the Lewis rat. In rats with tail weakness due to acute PLP-EAE, conduction was normal in the spinal nerve roots and peripheral nerves but there was evidence of conduction block in a high proportion of the fibres in the dorsal columns of the lumbosacral spinal cord. In contrast, in acute MBP-EAE, there was conduction block in a high proportion of fibres in the sacral dorsal and ventral roots of the peripheral nervous system (PNS) and in the dorsal columns of the lumbosacral spinal cord. The distribution of nerve conduction abnormalities is consistent with previous histological studies showing that inflammation and primary demyelination are restricted to the CNS in PLP-EAE, but are present in the CNS and in the spinal roots of the PNS in MBP-EAE. The restriction of functional abnormalities to the CNS in PLP-EAE but not in MBP-EAE may have implications for the human inflammatory demyelinating diseases, including multiple sclerosis.

show abstract

Section: Discussionsupporting

confidence: 89%

Conduction abnormalities are restricted to the central nervous system in experimental autoimmune encephalomyelitis induced by inoculation with proteolipid protein but not with myelin basic protein

Chalk

McCombe

Pender

1994

Brain

View full text Add to dashboard Cite

show abstract

“…Furthermore, MBP-specific T cell blasts from SAME animals did not react with inactivated measles virus and conversely the MV-specific line failed to proliferate in the presence of MBP suggesting that molecular mimicry between measles virus proteins and rat MBP is unlikely. However, it cannot be ruled out that cross-reactivity exists between measles virus and other potentially encephalitogenic proteins such as the proteolipid protein (Williams et al, 1982;Yoshimura et al, 1985) which do not have any sequences homologous to MBP.…”

Section: Discussionmentioning

confidence: 99%

Induction of autoimmune reactions to myelin basic protein in measles virus encephalitis in Lewis rats

Liebert¹,

Linington²,

Meulen³

1988

Journal of Neuroimmunology

View full text Add to dashboard Cite

Intracerebral inoculation of weanling Lewis rats with measles virus led to the development of subacute measles encephalomyelitis (SAME) 4-8 weeks after infection. The disease is characterized pathologically by an intense inflammatory infiltration within both the white and grey matter of the central nervous system (CNS) without apparent demyelination. Both during and after SAME splenic lymphocytes from these animals could be restimulated in vitro to proliferate in the presence of myelin base protein (MBP). MBP-specific class II MHC-restricted T cell lines were isolated from this cell population. They were shown to exhibit no cross-reactivity with measles virus and to induce experimental allergic encephalitis (EAE) in naive syngeneic recipients following adoptive transfer. The clinical and histopathological signs of this T cell-mediated disease were identical to that seen in classical T cell-mediated EAE. A humoral immune response to MBP was only detected in a limited number of those rats with SAME. These results indicate that autoimmune reactions to brain antigen can arise during measles virus infection which may contribute to the pathogenesis of measles virus-associated encephalomyelitis.

show abstract

“…Experimental autoimmune encephalomyelitis (EAE) is one of the most well-established and intensively studied animal models of human MS. EAE is either induced by active immunization with brain antigens emulsified in complete Freund’s adjuvant [4] or by adoptive transfer of brain antigen-specific T cells [5]. Various antigens are commonly used to induce EAE, for example, myelin basic protein (MBP) [6], myelin oligodendrocyte glycoprotein (MOG) [7], or proteolipid protein (PLP) [8]. …”

Section: Eae—an Animal Model Of Ms and Tissue-specific Autoimmunitymentioning

confidence: 99%

Knocking at the brain’s door: intravital two-photon imaging of autoreactive T cell interactions with CNS structures

Kawakami

Flügel

2010

Semin Immunopathol

View full text Add to dashboard Cite

Since the first applications of two-photon microscopy in immunology 10 years ago, the number of studies using this advanced technology has increased dramatically. The two-photon microscope allows long-term visualization of cell motility in the living tissue with minimal phototoxicity. Using this technique, we examined brain autoantigen-specific T cell behavior in experimental autoimmune encephalitomyelitis, the animal model of human multiple sclerosis. Even before disease symptoms appear, the autoreactive T cells arrive at their target organ. There they crawl along the intraluminal surface of central nervous system (CNS) blood vessels before they extravasate. In the perivascular environment, the T cells meet phagocytes that present autoantigens. This contact activates the T cells to penetrate deep into the CNS parenchyma, where the infiltrated T cells again can find antigen, be further activated, and produce cytokines, resulting in massive immune cell recruitment and clinical disease.

show abstract

Chronic Experimental Allergic Encephalomyelitis Induced in Rabbits with Bovine White Matter Proteolipid Apoprotein

Cited by 66 publications

References 0 publications

Conduction abnormalities are restricted to the central nervous system in experimental autoimmune encephalomyelitis induced by inoculation with proteolipid protein but not with myelin basic protein

Conduction abnormalities are restricted to the central nervous system in experimental autoimmune encephalomyelitis induced by inoculation with proteolipid protein but not with myelin basic protein

Induction of autoimmune reactions to myelin basic protein in measles virus encephalitis in Lewis rats

Knocking at the brain’s door: intravital two-photon imaging of autoreactive T cell interactions with CNS structures

Contact Info

Product

Resources

About