Chronic Exposure to 4-Nonylphenol Alters UDP-Glycosyltransferase and Sulfotransferase Clearance of Steroids in the Hard Coral, Pocillopora damicornis

Rougée, L.; Collier, Abby C.; Richmond, Robert H.

doi:10.3389/fphys.2021.608056

Cited by 6 publications

(4 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, Meng et al (2022) tested human urinalysis strips on corals and found that ketone and leukocytes were reliably detected by the strips. Some of the proteins that were upregulated in corals exposed to reduced water quality, particularly toxicants, such as pesticides and polycyclic aromatic hydrocarbons (PAHs) ubiquitously detected in this study, are steroidal clearance/detoxification and regeneration enzymes that also play a role in reproduction (Rougée et al, 2006;Rougée et al, 2015;Rougée et al, 2021). Just as coral colony energetic allocation to maintenance and repair can compromise reproductive success including gamete quality and fecundity, so too can the allocation of key proteins to detoxification reduce the availability for reproduction and hence, population replenishment.…”

Section: Discussionmentioning

confidence: 91%

Differential molecular biomarker expression in corals over a gradient of water quality stressors in Maunalua Bay, Hawaii

Tisthammer,

Martinez,

Downs

et al. 2024

Front. Physiol.

Self Cite

View full text Add to dashboard Cite

Coral reefs globally face unprecedented challenges from anthropogenic stressors, necessitating innovative approaches for effective assessment and management. Molecular biomarkers, particularly those related to protein expressions, provide a promising avenue for diagnosing coral health at the cellular level. This study employed enzyme-linked immunosorbent assays to evaluate stress responses in the coral Porites lobata along an environmental gradient in Maunalua Bay, Hawaii. The results revealed distinct protein expression patterns correlating with anthropogenic stressor levels across the bay. Some proteins, such as ubiquitin and Hsp70, emerged as sensitive biomarkers, displaying a linear decrease in response along the environmental gradient, emphasizing their potential as indicators of stress. Our findings highlighted the feasibility of using protein biomarkers for real-time assessment of coral health and the identification of stressors. The identified biomarkers can aid in establishing stress thresholds and evaluating the efficacy of management interventions. Additionally, we assessed sediment and water quality from the inshore areas in the bay and identified organic contaminants, including polycyclic aromatic hydrocarbons and pesticides, in bay sediments and waters.

show abstract

Section: Discussionmentioning

confidence: 91%

Differential molecular biomarker expression in corals over a gradient of water quality stressors in Maunalua Bay, Hawaii

Tisthammer,

Martinez,

Downs

et al. 2024

Front. Physiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Studies with an unclear risk of bias showed no significant effect of 4-nonylphenol on several parameters such as the release of Poc. damicornis planulae, the content in cholesterol, the content in steroids (estrone, estradiol, testosterone and progesterone), and the activity of several enzymes such as the 3-beta-hydroxysteroid dehydrogenase, the cytochrome P450, the glutathione-S-transferase, and the beta-glucuronidase [75]. However, there was an increase in the activity of UDP-glycosyltransferase and a decrease in sulfotransferase 1A1 activity [75].…”

Section: Narrative Synthesis Of Study Findingsmentioning

confidence: 99%

“…damicornis planulae, the content in cholesterol, the content in steroids (estrone, estradiol, testosterone and progesterone), and the activity of several enzymes such as the 3-beta-hydroxysteroid dehydrogenase, the cytochrome P450, the glutathione-S-transferase, and the beta-glucuronidase [75]. However, there was an increase in the activity of UDP-glycosyltransferase and a decrease in sulfotransferase 1A1 activity [75]. Dispersant In the dispersant category, the exposures recorded in the narrative synthesis were also recorded in the quantitative synthesis, and only one study, which showed no statistically significant effect of Corexit 9527 on polyp retraction of Pseudodiploria strigosa, has a low risk of bias [76] (Table 9).…”

Section: Narrative Synthesis Of Study Findingsmentioning

confidence: 99%

What are the toxicity thresholds of chemical pollutants for tropical reef-building corals? A systematic review

et al. 2023

View full text Add to dashboard Cite

Background Tropical coral reefs cover only ca. 0.1% of the Earth’s surface but harbour exceptional marine biodiversity and provide vital ecosystem services to millions of people living nearby. They are currently threatened by global (e.g. climate change) and local (e.g. chemical pollution) stressors that interact in multiple ways. While global stressors cannot be mitigated by local actions alone, local stressors can be reduced through ecosystem management. Here, we aimed to systematically review experimental studies assessing the toxicity of chemical pollutants to tropical reef-building corals to generate accessible and usable knowledge and data that can be used to calculate measurement endpoints in ecological risk assessment. From the quantitative estimates of effects, we determined toxicity thresholds as the highest exposures tested at which no statistically significant adverse effects were observed, and we compared them to regulatory predicted no effect concentrations for the protection of marine organisms, to assess whether these reference values are indeed protective of corals. Methods The evidence was taken from a systematic map of the impacts of chemicals arising from human activity on tropical reef-building corals published in 2021. All studies in the map database corresponding to the knowledge cluster “Evidence on the ecotoxicological effects of chemicals on corals” were selected. To identify subsequently published literature, the search was updated using a subset of the search string used for the systematic map. Titles, abstracts and full-texts were screened according to the criteria defining the selected cluster of the map. Because the eligibility criteria for the systematic review are narrower than the criteria used to define the cluster in the systematic map, additional screening was performed. Studies included were critically appraised and each study was rated as low, unclear, medium, or high risk of bias. Data were extracted from the studies and synthesised according to a strategy dependent on the type of exposure and outcome. Review findings The systematic review reports the known effects of chemical exposures on corals from 847 studies corresponding to 181 articles. A total of 697 studies (161 articles) were included in the quantitative synthesis and 150 studies (50 articles) in the narrative synthesis of the findings. The quantitative synthesis records the effects of 2706 exposure concentrations-durations of 164 chemicals or mixtures of chemicals, and identifies 105 toxicity thresholds corresponding to 56 chemicals or mixtures of chemicals. When toxicity thresholds were compared to reference values set for the protection of marine organisms by environmental agencies, the reference values appear to be protective of corals for all but three chemicals assessed: the metal copper and the pesticides diuron and irgarol 1051. Conclusions This open-access database of known ecotoxicological effects of chemical exposures on corals can assist managers in the ecological risk assessment of chemicals, by allowing easy determination of various ecotoxicological thresholds. Several limitations of the toxicity tests synthesised here were noted (in particular the lack of measurement of effective concentrations for more than half of the studies). Overall, most of the currently available data on coral toxicity should be replicated independently and extended to corals from less studied geographical regions and functional groups.

show abstract

“…Nonylphenol has an estimated half-life of 58 days within aquatic environments but this timeline can be accelerated in the presence of sunlight (Rougée et al, 2021). Although this may seem short, as NP readily binds organic matter, it can be absorbed into soil and sediment with an estimated half-life of over 60 years.…”

Section: Nonylphenol Degradationmentioning

confidence: 99%

The Study of SELEX Methodology in the Generation of Aptamers for Environmental Contaminant Nonylphenol

Mullan¹

View full text Add to dashboard Cite

Nonylphenol is an emerging organic contaminant released into the environment by anthropogenic activity. Nonylphenol is a mix of 211 branched-chain isomers collectively referred to as nonylphenol technical equivalents (NPTE), with the main isomer being 4-nonylphenol (4-NP). These isomers are capable of interfering with developmental and physiological signalling in humans, as well as numerous other terrestrial and aquatic species. Its presence has been documented in sediment and water bodies worldwide, including Aotearoa New Zealand. Due to the current limitations of environmental monitoring, researchers are becoming increasingly motivated to develop alternative techniques for detecting contaminants. Aptamer-based biosensors are of interest as their low cost, ease of use and quick analyses times meets the requirements for frequent monitoring. Aptamers are synthetic DNA molecules which provide the molecular-recognition element to biosensors. This is derived from their ability to fold into complex three-dimensional structures and form non-covalent bonds with their target molecule. Aptamers are developed through an in vitro process called systematic evolution of ligands by exponential enrichment (SELEX). The aim of this Masters study was to generate aptamers that bound single (4-NP) or multiple (NPTE) nonylphenol isomers. To achieve this, two SELEX experiments were performed against each target molecule(s) using a modified version of traditional SELEX methodology with additional counter- and negative-selection steps. Within each SELEX experiment, stringency was increased at each round in the form of Tween 20 detergent, starting at low (0.01% v/v) or high (0.1% v/v) concentrations to determine their effects on the libraries being enriched with sequences that bind either 4-NP or NPTE. Real-time PCR was used to monitor DNA quantity across selection rounds. Following this, four libraries were sequenced using Illumina sequencing and analysed using an aptamer-specific bioinformatics pipeline. The targetbinding ability of ten candidates from each library were determined using an existing characterisation technique utilising gold nanoparticles (AuNPs). Prior to characterisation, due to the nature of the target molecule, preliminary studies were conducted to assess the effects of three organic solvents on the AuNP assay. The results of this study demonstrated that the higher stringency conditions led to reduced DNA quantity and overall fewer unique sequences in the final library. Bioinformatics data demonstrated that the top clusters in libraries with sequences enriched for 4-NP under high stringency conditions, compared to low stringency conditions, contained a greater range of unique sequences. Overall, more clusters were found in the NPTE library following high stringency selection compared to both 4-NP libraries, though it is unclear whether this was derived from non-specific binding. Results from the AuNP characterisation assay showed no aptamer candidates bound to either 4-NP or NPTE, although an additional common characterisation method should also be used. Given that no aptamers were identified to bind any of the nonylphenyl isomers, the effects of stringency conditions and multi- or singletarget SELEX on aptamer characteristics could not be assessed. However, some insights from this work including the effect of detergent concentration on library populations and the use of solvents in the AuNP assay may be used to advise future experimental design for small molecule aptamer development.

show abstract

Chronic Exposure to 4-Nonylphenol Alters UDP-Glycosyltransferase and Sulfotransferase Clearance of Steroids in the Hard Coral, Pocillopora damicornis

Cited by 6 publications

References 60 publications

Differential molecular biomarker expression in corals over a gradient of water quality stressors in Maunalua Bay, Hawaii

Differential molecular biomarker expression in corals over a gradient of water quality stressors in Maunalua Bay, Hawaii

What are the toxicity thresholds of chemical pollutants for tropical reef-building corals? A systematic review

The Study of SELEX Methodology in the Generation of Aptamers for Environmental Contaminant Nonylphenol

Contact Info

Product

Resources

About