Chronic exposure to chlorpyrifos triggered body weight increase and memory impairment depending on human apoE polymorphisms in a targeted replacement mouse model

Peris-Sampedro, Fiona; Basaure, Pia; Reverte, Ingrid; Cabré, María; Domingo, José L.; Colomina, María Teresa

doi:10.1016/j.physbeh.2015.03.006

Cited by 33 publications

(15 citation statements)

References 78 publications

(96 reference statements)

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“…The present investigation indicated that repeated exposure to CPF induced a weight gain in both apoE3 and C57BL/ 6N mice. Nevertheless, this increase was faster and steeper in ε3 carriers, in agreement with our previous study (Peris-Sampedro et al, 2015). The present results also revealed an increase in leptin levels in exposed apoE3 animals.…”

Section: Discussionsupporting

confidence: 94%

“…Together with recent results (Peris-Sampedro et al, 2015), the current data provide enough evidence to suggest that human apoE3 isoform expression increases vulnerability to developing obesity and related metabolic dysfunctions after CPF exposure. Although not conclusive, the results of this study suggest that CPF has hormonal targets, such as leptin or ghrelin, on which, to date, little has been reported in the scientific literature.…”

Section: Discussionsupporting

confidence: 76%

“…Our results indicated that repeated exposure to CPF generally increased food ingestion. In line with these findings, we recently found that CPF exposure tended to alter feeding behavior (Peris-Sampedro et al, 2015). In relation to this, the role of ghrelin deserves special mention.…”

Section: Discussionsupporting

confidence: 71%

“…While apoE3 is accepted as the healthy phenotype, recent experimental data have shown that it tends to be more prone to developing diet-induced obesity (ArbonesMainar et al, 2008;Huebbe et al, 2015;Karagiannides et al, 2008), and more vulnerable to decabromodiphenyl ether (Reverte et al, 2013). In a recent study, we found that apoE3 mice were more vulnerable to gain excess weight upon CPF exposure than apoE2 and apoE4 mice (Peris-Sampedro et al, 2015).…”

Section: Introductionmentioning

confidence: 49%

“…Standard rodent chow (Panlab, Barcelona, Spain) was supplemented with CPF at a concentration intended to deliver a dose of 2 mg/kg body weight/day, based on the results of our recent study (Peris-Sampedro et al, 2015). The protease inhibitor 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF) was also purchased from Sigma-Aldrich.…”

Section: Chemicalsmentioning

confidence: 99%

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Adulthood dietary exposure to a common pesticide leads to an obese-like phenotype and a diabetic profile in apoE3 mice

Peris-Sampedro¹,

Cabré

Basaure³

et al. 2015

Environmental Research

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 76%

Section: Discussionsupporting

confidence: 71%

Section: Introductionmentioning

confidence: 49%

Section: Chemicalsmentioning

confidence: 99%

See 3 more Smart Citations

Adulthood dietary exposure to a common pesticide leads to an obese-like phenotype and a diabetic profile in apoE3 mice

Peris-Sampedro¹,

Cabré

Basaure³

et al. 2015

Environmental Research

Self Cite

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New mechanistic insights on the metabolic-disruptor role of chlorpyrifos in apoE mice: a focus on insulin- and leptin-signalling pathways

et al. 2018

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Recently, we have provided evidence, suggesting that mice expressing the human apolipoprotein E3 (apoE3) are more prone to develop an obesity-like phenotype and a diabetic profile when subchronically fed a chlorpyrifos (CPF)-supplemented diet. The aim of the current study was to examine the underlying mechanisms through which CPF alters both insulin- and leptin-signalling pathways in an APOE-dependent manner. Both adult apoE3- and E4-targeted replacement and C57BL/6 mice were exposed to CPF at 0 or 2 mg/kg body weight/day through the diet for 8 consecutive weeks. We determined the expression of JAK2, p-JAK2, STAT3, p-STAT3, SOCS3, IRS-1, p-IRS-1, AKT, p-AKT, GSK3β, p-GSK3β, and apoE in the liver, as well as hepatic mRNA levels of pon1, pon2, and pon3. CPF markedly disrupted both leptin and insulin homeostasis, particularly in apoE3 mice. Indeed, only CPF-fed apoE3 mice exhibited an increased phosphorylation ratio of STAT3, as well as increased total SOCS3 protein levels. Similarly, the exposure to CPF drastically reduced the phosphorylation ratio of both AKT and GSK3β, especially in apoE3 mice. Overall, CPF reduced the expression of the three pon genes, principally in C57BL/6 and apoE3 mice. These results provide notable mechanistic insights on the metabolic effects of the pesticide CPF, and attest the increased vulnerability of apoE3 carriers to its metabolic-disruptor role.

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Learning, memory and the expression of cholinergic components in mice are modulated by the pesticide chlorpyrifos depending upon age at exposure and apolipoprotein E (APOE) genotype

et al. 2019

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Chronic exposure to chlorpyrifos triggered body weight increase and memory impairment depending on human apoE polymorphisms in a targeted replacement mouse model

Cited by 33 publications

References 78 publications

Adulthood dietary exposure to a common pesticide leads to an obese-like phenotype and a diabetic profile in apoE3 mice

Adulthood dietary exposure to a common pesticide leads to an obese-like phenotype and a diabetic profile in apoE3 mice

New mechanistic insights on the metabolic-disruptor role of chlorpyrifos in apoE mice: a focus on insulin- and leptin-signalling pathways

Learning, memory and the expression of cholinergic components in mice are modulated by the pesticide chlorpyrifos depending upon age at exposure and apolipoprotein E (APOE) genotype

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