Chronic exposure to complex metal oxide nanomaterials induces production of reactive oxygen species in bacteria

Abstract

Use of complex metal oxide nanoparticles has drastically risen in recent years, especially due to their utility in electric vehicle batteries. However, use of these materials has outpaced our understanding...

Colloidal stabilization of hydrophobic InSe 2D nanosheets in a model environmental aqueous solution and their impact on Shewanella oneidensis MR-1

“…While generally less sensitive than fluorescence techniques, this amperometric method was used successfully to detect micromolar levels of H 2 O 2 formed due to the REDOX transformations of lithium cobalt oxide (LCO) battery materials. 38,39 In our experiments, adding H 2 O 2 to a final concentration of 15 μM in 5 mM EGCG solution increased the current compared to the background current signal when the electrode was immersed in a 5 mM EGCG solution by about 50%. This signal increase indicated that the amperometric detection method was sensitive to the presence of H 2 O 2 in the solution.…”

Understanding the reaction mechanisms of nicotinamide adenine dinucleotide (NADH) with lithium cobalt oxide and other metal oxide nanomaterials

“…Examples include flavin adenine dinucleotide (FAD) and related flavins, 53,54 DNA, and RNA. While DNA oxidation has been reported in response to nanoparticle exposure, 23,24 the detailed molecular origins remain unknown. Studies like those reported here may provide key insights into the fundamental chemical origins of nanoparticle interactions with living systems in the environment.…”

“…However, studies of LiCoO 2 and related molecules under laboratory conditions have frequently observed significant release of Co at concentrations vastly exceeding the aforementioned values. 15,23,24 As a result, it can be concluded that the oxidation-reduction chemistry must also be taking place, with the metal oxide being reduced by one or more solution components. We recently showed that NADH, an important molecule in cellular respiration, interacts strongly with LiCoO 2 via oxidation-reduction (redox) chemistry.…”

Modern materials provoke ancient behavior: bacterial resistance to metal nanomaterials

“…95 Finally, ROS generated during exposures has been identified to increase transformation and mutation Environmental Science: Nano Perspective frequency. 23,96 Thus, the use of ENMs may promote the spread of antibiotic resistance in hospitals and contaminated environmental sites. 97 ENMs are under consideration as emerging pollutants, so action must be taken to provide regulations and guidance for their appropriate disposal and recycling.…”

“…20 ENM redox properties can cause an oxidative stress response, in addition to exogenous reactive oxygen species (ROS) generation by dissolution of the particles themselves (through Fenton, Fenton-like, Haber Weiss, or light-initiated processes). [21][22][23] ROS contributions to ENM toxicity are difficult to assess due to their apparent non-preferential targeting of biomolecules. In addition, oxidative damage is likely to disturb the function and expression of other systems, making it extremely difficult to map the initial targets of ENM action in comparison to the subsequent effects.…”