Chronic fluoxetine exposure alters movement and burrowing in adult freshwater mussels

Hazelton, Peter D.; Du, Bowen; Haddad, Samuel P.; Fritts, Andrea K.; Chambliss, C. Kevin; Brooks, Bryan W.; Bringolf, Robert B.

doi:10.1016/j.aquatox.2013.12.019

Cited by 63 publications

(46 citation statements)

References 55 publications

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“…In the present study, fluoxetine, norfluoxetine, sertraline and desmethylsertraline were detected in snail tissues, but not detected in water, potentially because SSRIs appear to sorb strongly to suspended particulates in surface waters (Baker and Kasprzyk-Hordern, 2011), which would have been removed during a prefiltering step prior to extraction and analysis methods in the present study. Thus, based on observations of the present study and more recent observations across trophic positions (Du et al, 2014b), water exposure may be more important for fish, but perhaps not filter feeding invertebrates (e.g., bivalves) (Franzellitti et al, 2014;Hazelton et al, 2014), than diet. Though the current study was not designed to study other trophic positions or relevant trophic transfer of target pharmaceuticals in wadeable streams, the occurrence of select pharmaceuticals in periphyton and Planorbid sp.…”

Section: Pharmaceutical Bioaccumulation By Periphyton and Invertebratmentioning

confidence: 78%

Pharmaceutical bioaccumulation by periphyton and snails in an effluent-dependent stream during an extreme drought

Haddad

Scott

et al. 2015

Chemosphere

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Section: Pharmaceutical Bioaccumulation By Periphyton and Invertebratmentioning

confidence: 78%

Pharmaceutical bioaccumulation by periphyton and snails in an effluent-dependent stream during an extreme drought

Haddad

Scott

et al. 2015

Chemosphere

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“…For example, SSRI exposure slowed predator avoidance behaviors in fathead minnows (Painter et al, 2009;Weinberger and Klaper, 2014) and Arabian killifish (Aphanius dispar) (Barry, 2013), reduced anxietylike behaviors in zebrafish (Wong et al, 2013), increased lethargy in western mosquitofish (Gambusia affinis) (Henry and Black, 2008) and reduced swimming activity in Arabian killifish (Barry, 2013) and sheepshead minnow (Winder et al, 2012). SSRI effects on locomotory behavior have also been observed in several invertebrate species (Fong and Ford, 2014;Hazelton et al, 2014). However, although SSRIs were also reported to reduce the ability of hybrid striped bass to capture prey possibly due to decreased locomotion (Gaworecki and Klaine, 2008;Bisesi et al, 2014), there is currently insufficient evidence to support the notion that reduced food intake caused by SSRIs is mediated through its effects specifically on locomotory abilities.…”

Section: Description Of Aop For Growth Impairment By Ssrismentioning

confidence: 89%

Development and application of the adverse outcome pathway framework for understanding and predicting chronic toxicity: II. A focus on growth impairment in fish

et al. 2015

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Adverse outcome pathways (AOPs) organize knowledge on the progression of toxicity through levels of biological organization. By determining the linkages between toxicity events at different levels, AOPs lay the foundation for mechanism-based alternative testing approaches to hazard assessment. Here, we focus on growth impairment in fish to illustrate the initial stages in the process of AOP development for chronic toxicity outcomes. Growth is an apical endpoint commonly assessed in chronic toxicity tests for which a replacement is desirable. Based on several criteria, we identified reduction in food intake to be a suitable key event for initiation of middle-out AOP development. To start exploring the upstream and downstream links of this key event, we developed three AOP case studies, for pyrethroids, selective serotonin reuptake inhibitors (SSRIs) and cadmium. Our analysis showed that the effect of pyrethroids and SSRIs on food intake is strongly linked to growth impairment, while cadmium causes a reduction in growth due to increased metabolic demands rather than changes in food intake. Locomotion impairment by pyrethroids is strongly linked to their effects on food intake and growth, while for SSRIs their direct influence on appetite may play a more important role. We further discuss which alternative tests could be used to inform on the predictive key events identified in the case studies. In conclusion, our work demonstrates how the AOP concept can be used in practice to assess critically the knowledge available for specific chronic toxicity cases and to identify existing knowledge gaps and potential alternative tests.

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“…However, many other endpoints could be explored, and some may provide more insight into effects from chronic exposure. Relevant toxicological endpoints in sublethal studies of freshwater mussel sensitivity to other contaminants that may be applied in future fluridone and endothall studies include growth (in juveniles, Bringolf et al 2007;Wang et al 2007Wang et al , 2011Wang et al , 2013, glochidial metamorphosis success (Hazelton et al 2013), female mantle lure display (Bringolf et al 2010;Hazelton et al 2013;Leonard 2013), hemolymph and tissue analysis (Archambault et al 2013;Leonard 2013), movement and burrowing (Flynn & Spellman 2009;Archambault et al 2013;Hazelton et al 2014), and metabolomics (Leonard 2013). We attempted to evaluate female mantle lure display in our experiment, but we had few females per replicate, thus there was insufficient statistical power to make sound inferences.…”

Section: Discussionmentioning

confidence: 99%

Sensitivity of freshwater molluscs to hydrilla-targeting herbicides: providing context for invasive aquatic weed control in diverse ecosystems

Archambault

Bergeron

Cope

et al. 2014

Journal of Freshwater Ecology

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Hydrilla (Hydrilla verticillata) is an invasive aquatic weed that has spread rapidly throughout the USA, especially in the southeast. A common control method is the application of aquatic herbicides, such as fluridone and endothall. However, there is limited documentation on the effects of herbicides commonly used to control hydrilla and other aquatic weeds on many non-target freshwater species and no published information exists on the toxicity of these herbicides to freshwater molluscs. We exposed juveniles (96 h) and glochidia (48 h) of the unionid mussel Lampsilis siliquoidea and adults (28 d) of Lampsilis fullerkati to a formulation of fluridone (Sonar À PR Ò ) in laboratory toxicity tests. The early life stages of L. siliquoidea were also exposed to a formulation of the dipotassium salt of endothall (Aquathol À K Ò ) in separate tests. Juveniles of the freshwater gastropod snail, Somatogyrus viriginicus (Lithoglyphidae), were exposed (96 h) to the Sonar À Genesis Ò fluridone formulation. Endpoints were survival (all species and life stages) as well as siphoning behavior and foot protrusion (adult mussels). Median lethal fluridone concentrations (LC50s) were 865 mg/L (95% CI, 729À1,026 mg/L) for glochidia (24 h), 511 mg/L (309À843 mg/L) for juvenile L. siliquoidea (96 h), and 500 mg/L (452À553 mg/L) for juvenile S. viriginicus (96 h). No mortality occurred in the 28-d exposure of adult L. fullerkati and we found no statistically significant effect of fluridone concentration on foot protrusion (p D 0.06) or siphoning behavior (p D 0.08). The 24-h LC50 for glochidia exposed to the dipotassium salt of endothall was 31.2 mg/L (30.3À32.2 mg/L) and the 96-h LC50 for juvenile mussels was 34.4 mg/L (29.3À40.5 mg/L). Freshwater molluscs were more sensitive to fluridone and endothall than most other species previously tested. Fluridone and endothall concentrations typically recommended for hydrilla treatment (5À15 mg/L and 1À5 mg/L, respectively) were not acutely toxic to the molluscs we tested and a 28-d exposure to fluridone was not lethal to adult mussels even at the highest concentration (300 mg/L), indicating minimal risk of short-term exposure effects.

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Chronic fluoxetine exposure alters movement and burrowing in adult freshwater mussels

Cited by 63 publications

References 55 publications

Pharmaceutical bioaccumulation by periphyton and snails in an effluent-dependent stream during an extreme drought

Pharmaceutical bioaccumulation by periphyton and snails in an effluent-dependent stream during an extreme drought

Development and application of the adverse outcome pathway framework for understanding and predicting chronic toxicity: II. A focus on growth impairment in fish

Sensitivity of freshwater molluscs to hydrilla-targeting herbicides: providing context for invasive aquatic weed control in diverse ecosystems

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