Chronic granulomatous disease presenting with small bone osteomyelitis in a young child: A case report

Chari, Preethi S.; Chandra, P. K.; Prasad, S Rajendra; Bhattad, Sagar

doi:10.4103/ijpm.ijpm_458_17

Cited by 3 publications

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, any abnormalities or diseases that affect phagocytic activity in both inflammatory and bacterial processes could be studied using the NBT reduction rate. In human medicine, the NBT reduction rate has been used in numerous cases such as chronic granulomatous diseases, 12,13 tuberculosis, 14 viral meningoencephalitis, 15 and liver abscesses caused by amebiasis 16 . Recently, the NBTT has also been used in several other cases, such as to investigate the immune profile of human patients with fungus infection, 17 gingival fibromatosis, 18 chronic obstructive pulmonary disease, 19 and ROS production during HL‐60 cell differentiation, 20 in biofilms, 21 and during heat‐stressed whole blood cultures 22 …”

Section: Discussionmentioning

confidence: 99%

Effect of storage on the nitro blue tetrazolium reduction test in dog blood samples

Baxarias,

Solano‐Gallego

2023

Veterinary Clinical Pathol

View full text Add to dashboard Cite

IntroductionThe nitro blue tetrazolium (NBT) reduction test (NBTT) has been used for measuring the metabolic activity of phagocytes of mammals. Activated neutrophils transform NBT into formazan in the cytoplasm. The NBTT can detect the activation of neutrophils in peripheral blood and is used to assess neutrophil function in dogs. However, the NBTT is not used frequently in the clinical setting, as samples should be processed after blood collection.ObjectiveThe aim of this study was to evaluate the effect of storage on NBTT in dog blood samples.Materials and MethodsResidual EDTA blood samples from 22 dogs were included of different ages, breeds, and sex. The buffy coat layer was separated from the blood and incubated with 0.1% NBT. The NBTT was performed at 0, 24, 48, and 72 h after the collection of blood. Blood samples were stored at 4°C until the tests were performed. Blood smears were evaluated by light microscopy, and the NBT reduction rate was reported, which represents the percentage of activated neutrophils. The NBT reduction rate was calculated after counting 300 neutrophils in each slide.ResultsThe means of NBTT in dog blood samples at 0, 24, 48, and 72 h were 8.3%, 8.5%, 8.7%, and 7.8%, respectively. No significant differences were observed between time points.ConclusionsThis study showed that the NBTT can be performed up to 72 h after the collection of canine blood if correctly refrigerated at 4°C. This finding supports the performance of the NBTT in the clinical setting.

show abstract