Chronic impact of sulfamethoxazole on the metabolic activity and composition of enriched nitrifying microbial culture

Katipoglu‐Yazan, Tugce; Merlin, Christophe; Pons, Marie‐Noëlle; Ubay‐Cokgor, Emine; Orhon, Derin

doi:10.1016/j.watres.2016.05.043

Cited by 48 publications

(39 citation statements)

References 38 publications

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“…Acclimation phase was monitored with conventional parameters, respirometry, and molecular methods. The results related to nitrifier abundances and gene activities were reported elsewhere . This study targeted monitoring the change of nitrifier process kinetics and overall evaluation of chronic SMX dosing.…”

Section: Experimental Developmentmentioning

confidence: 99%

“…The SMX acclimation reactor was started by feeding the same substrate mixture including additional 50 mg L ‐1 SMX antibiotic in the final volume of the same activated sludge system. The monitoring results of acclimation reactor were reported elsewhere …”

Section: Experimental Developmentmentioning

confidence: 99%

“…The adverse effects of SMX were mainly investigated with the comparison of process kinetics and the change of microbial composition in carbon removal systems where the microbial community was dominated by heterotrophic bacteria . However, research on the impact of SMX on the nitrification mechanism is limited to a few studies …”

Section: Introductionmentioning

confidence: 99%

“…Universal genes coding for 16S rRNA of specific NOB has been used in environmental studies instead of functional genes . Recently, the adverse effects of SMX have been investigated at amoA and specific 16S rRNA gene/gene transcript level . However, accurate interpretation of the interactions between chemicals and microbial community requires establishing the basic relationships between process kinetics, metabolic activity and composition of microbial community …”

Section: Introductionmentioning

confidence: 99%

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Chronic impact of sulfamethoxazole: how does process kinetics relate to metabolic activity and composition of enriched nitrifying microbial culture?

Katipoglu‐Yazan

Ubay‐Cokgor

Orhon

2018

J of Chemical Tech & Biotech

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BACKGROUND This study investigated utilization kinetics of ammonia‐nitrogen induced by chronic sulfamethoxazole (SMX) exposure on biomass with an enriched nitrifying community. A lab‐scale activated sludge system was supplied with 100 mg COD L‐1 of peptone mixture and 50 mg N L‐1 of ammonia nitrogen at a SRT of 15 days. At steady‐state, the reactor was operated with additional daily SMX dosing of 50 mg L‐1 for 35 days. Profiles of oxygen uptake rates and nitrogen fractions obtained in respirometric/batch experiments were used for estimation of nitrification kinetics. RESULTS Acute inhibitory impact of SMX was expressed with high levels of half saturation constants and endogenous decay rates. After 35 days, half saturation constants significantly increased while higher active biomass fractions and recovered endogenous decay rates were estimated. At the end of the acclimation phase, 92% nitrification was achieved with 10 mg L‐1 SMX utilization. CONCLUSION Interpretation of modeling results with the outcomes of molecular analysis facilitated explanation of autotrophic/heterotrophic bacteria behavior in the course of SMX exposure. Removal of SMX may be attributed to co‐metabolism with ammonia oxidation and/or activity of SMX utilizing heterotrophic bacteria. © 2017 Society of Chemical Industry

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Section: Experimental Developmentmentioning

confidence: 99%

Section: Experimental Developmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Chronic impact of sulfamethoxazole: how does process kinetics relate to metabolic activity and composition of enriched nitrifying microbial culture?

Katipoglu‐Yazan

Ubay‐Cokgor

Orhon

2018

J of Chemical Tech & Biotech

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“…The qPCR amplification of 16rRNA functional genes was conducted with the following primers: 1055f/1391r (EUB) [13,14], CTO 189fA/B/RT1r (AOB) [15], Nspra-675f/746r (NOB) [16], and Amx809f/1066r (AnAOB) [17,18].…”

Section: Methodsmentioning

confidence: 99%

Decay Experiments of Effective N-Removing Microbial Communities in Sequencing Batch Reactors

Xing

Zhong

Wang

et al. 2017

Journal of Chemistry

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The temporal changes in the compositions of effective N-removing bacterial communities and the decay coefficients of Anammox were studied within the 120-day decay period under anaerobic or aerobic conditions at 25 ∘ C. The maximum nitrogen production rate (MNPR) was determined by measuring the temperature, pH, volatile suspended solids (VSSs), and nitrogen-removal efficiency of the microbial communities during the decay period. The decay coefficients under anaerobic and aerobic conditions at 25 ∘ C were determined through equation-based fitting to be 0.031 d −1 and 0.070 d −1 , respectively. Through molecular biological means and together with quantitative polymerase chain reaction (qPCR), the proportions of AnAOB in the microbial communities dropped from 48.70% to 3.69% under anaerobic condition and from 48.70% to 1.98% under aerobic condition during the decay period.

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How to eliminate the toxic impact of extreme salinity on the ecology of activated sludge? Experimental verification with pickle plant effluents

Övez

Özbasaran²,

Duba

et al. 2022

J of Chemical Tech & Biotech

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BACKGROUND:The study defined a sustainable management scheme to eliminate the toxic impact of extreme salinity on the ecology and performance of an activated sludge process. The scheme was implemented on a plant treating pickle plant effluents involving significant flow and salinity transients. It was conducted in two phases. Firstly, the plant was operated without attenuating extreme salinity transients, enabling observation of all adverse impacts on the microbial ecology. The second phase was implemented with a new management scheme tempering all salinity gradients revealing the recovery of the microbial ecology into a stable and sustainable state. RESULTS: Initially, the microbial community could not cope with rapid salinity increase and exhibited significant changes resulting in the predominance of filamentous microorganisms, disruption of the floc structure and almost total loss of eukaryotic microorganisms. Settling problems and deterioration of effluent quality were observed, followed by plasmolysis and repeated total loss of the biomass. The new waste management strategy allowed only transients limited to ±500 ∼S cm −1 in the influent; in this way, the microbial ecology steadily improved. The effluent chemical oxygen demand could be maintained below 80-90 mg L −1 with no appreciable particulate matter escape. CONCLUSIONS: Results identified sharp salinity transients as the key factor triggering total destruction of activated sludge. The novel scheme provided conclusive evidence that a stable microbial community could be maintained even when continuously exposed to a salinity level of around 10 000 ∼S cm −1 in the reactor, provided that variations remained limited to ±500 ∼S cm −1 , ensuring satisfactory effluent quality.

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Chronic impact of sulfamethoxazole on the metabolic activity and composition of enriched nitrifying microbial culture

Cited by 48 publications

References 38 publications

Chronic impact of sulfamethoxazole: how does process kinetics relate to metabolic activity and composition of enriched nitrifying microbial culture?

Chronic impact of sulfamethoxazole: how does process kinetics relate to metabolic activity and composition of enriched nitrifying microbial culture?

Decay Experiments of Effective N-Removing Microbial Communities in Sequencing Batch Reactors

How to eliminate the toxic impact of extreme salinity on the ecology of activated sludge? Experimental verification with pickle plant effluents

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