Chronic injection of pansomatostatin agonist ODT8-SST differentially modulates food intake and decreases body weight gain in lean and diet-induced obese rats

Stengel, Andreas; Coşkun, Tamer; Goebel-Stengel, Miriam; Craft, Libbey; Alsina‐Fernandez, Jorge; Wang, Lixin; Rivier, Jean; Taché, Yvette

doi:10.1016/j.regpep.2011.01.006

Cited by 5 publications

(4 citation statements)

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“…Conscious rats were placed lightly restrained (Ͻ1 min) in the holder tube of the quantitative nuclear magnetic resonance analysis apparatus (ECHO MRI, 3-1 Composition Analyzer; 150 Echo Medical Systems, Houston, TX). The body composition (fat mass, lean mass, and total water) was measured as described before (53).…”

Section: Measurementsmentioning

confidence: 99%

Orexigenic response to tail pinch: role of brain NPY₁ and corticotropin releasing factor receptors

Goebel-Stengel

Stengel

Wang

et al. 2014

American Journal of Physiology-Regulatory, Integrative and Comp

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Tail pinch stimulates food intake in rats. We investigated brain mechanisms of this response and the influence of repeated exposure. Sprague-Dawley rats received acute (5 min) or repeated (5 min/day for 14 days) tail pinch using a padded clip. Acute tail pinch increased 5-min food intake compared with control (0.92 ± 0.2 vs. 0.03 ± 0.01 g, P < 0.01). This response was inhibited by 76% by intracerebroventricular injection of BIBP-3226, a neuropeptide Y1 (NPY1) receptor antagonist, increased by 48% by astressin-B, a corticotropin-releasing factor (CRF) receptor antagonist, and not modified by S-406-028, a somatostatin subtype 2 antagonist. After the 5-min tail pinch without food, blood glucose rose by 21% (P < 0.01) while changes in plasma acyl ghrelin (+41%) and adrenocorticotropic hormone (+37%) were not significant. Two tail pinches (45 min apart) activate pontine and hindbrain catecholaminergic and hypothalamic paraventricular CRF neurons. After 14 days of repeated tail pinch, the 5-min orexigenic response was not significantly different from days 2 to 11 but reduced by 50% thereafter (P < 0.001). Simultaneously, the 5-min fecal pellet output increased during the last 5 days compared with the first 5 days (+58%, P < 0.05). At day 14, the body weight gain was reduced by 22%, with a 99% inhibition of fat gain and a 25% reduction in lean mass (P < 0.05). The orexigenic response to acute 5-min tail pinch is likely to involve the activation of brain NPY1 signaling, whereas that of CRF tends to dampen the acute response and may contribute to increased defecation and decreased body weight gain induced by repeated tail pinch.

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Section: Measurementsmentioning

confidence: 99%

Orexigenic response to tail pinch: role of brain NPY₁ and corticotropin releasing factor receptors

Goebel-Stengel

Stengel

Wang

et al. 2014

American Journal of Physiology-Regulatory, Integrative and Comp

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“…Body composition was assessed in conscious lightly restrained (ϳ1 min) rats using a quantitative nuclear magnetic resonance analysis apparatus (EchoMRI-700 Composition Analyzer; Echo Medical Systems, Houston, TX), as described before (49). Changes in fat mass, lean mass, and total water were determined by assessing body composition before and 2 wk after switching to standard or high-protein diets.…”

Section: Measurementsmentioning

confidence: 99%

High-protein diet selectively reduces fat mass and improves glucose tolerance in Western-type diet-induced obese rats

Stengel

Goebel-Stengel

Wang

et al. 2013

American Journal of Physiology-Regulatory, Integrative and Comp

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-Obesity is an increasing health problem. Because drug treatments are limited, diets remain popular. High-protein diets (HPD) reduce body weight (BW), although the mechanisms are unclear. We investigated physiological mechanisms altered by switching diet induced obesity (DIO) rats from Western-type diet (WTD) to HPD. Male rats were fed standard (SD) or WTD (45% calories from fat). After developing DIO (50% of rats), they were switched to SD (15% calories from protein) or HPD (52% calories from protein) for up to 4 weeks. Food intake (FI), BW, body composition, glucose tolerance, insulin sensitivity, and intestinal hormone plasma levels were monitored. Rats fed WTD showed an increased FI and had a 25% greater BW gain after 9 wk compared with SD (P Ͻ 0.05). Diet-induced obese rats switched from WTD to HPD reduced daily FI by 30% on day 1, which lasted to day 9 (Ϫ9%) and decreased BW during the 2-wk period compared with SD/SD (P Ͻ 0.05). During these 2 wk, WTD/HPD rats lost 72% more fat mass than WTD/SD (P Ͻ 0.05), whereas lean mass was unaltered. WTD/HPD rats had lower blood glucose than WTD/SD at 30 min postglucose gavage (P Ͻ 0.05). The increase of pancreatic polypeptide and peptide YY during the 2-h dark-phase feeding was higher in WTD/HPD compared with WTD/SD (P Ͻ 0.05). These data indicate that HPD reduces BW in WTD rats, which may be related to decreased FI and the selective reduction of fat mass accompanied by improved glucose tolerance, suggesting relevant benefits of HPD in the treatment of obesity. diet-induced obesity; food intake; high-protein diet; gut hormones OBESITY HAS BECOME EPIDEMIC, and by the year 2030, half of the adult population is predicted to be obese in the United States (54). Its prevalence is expanding worldwide in industrialized countries and spreading also to less developed countries; therefore "globesity" is taking over many parts of the world (see World Health Organization homepage). The plethora of health consequences related to obesity involves increased risk of developing adult-onset Type 2 diabetes mellitus, dyslipidemia, arteriosclerosis, and certain forms of cancer, including colorectal and pancreatic cancer (25). Therefore, obesity represents a major medical problem in the United States, and 12% of the entire health care costs are spent taking care of obesity-related comorbidities (52). A modest 5 to 10% body weight loss in obese subjects has been shown to result in improvements of these metabolic disturbances (21, 53).Although two antiobesity drugs have recently been approved, drug treatment options are still very limited (26), and therefore, dietary interventions remain popular. In particular, the use of a high-protein diet is considered a beneficial strategy to achieve a long-term reduction in body weight (8,22,47,48). However, the means by which an isocaloric high-protein diet reduces body weight are not well understood. There is some evidence that protein decreases appetite by increasing anorexigenic signaling (16,34,55) and stimulates thermogenesis (13,57,58) in experi...

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“…In all, this study adds to the growing literature interrogating the contributions of TN SST neurons to feeding behavior. Central SST (originally named growth hormone inhibiting hormone, GHIH, in the central nervous system, (Painson and Tannenbaum, 1991) had long been known to affect food intake through somatostatin receptor 2 (SSTR2; (Beranek et al, 1999; Campbell et al, 2017; Danguir, 1988; Lin et al, 1989; Andreas Stengel et al, 2010b, 2010a; A Stengel et al, 2010; Stengel et al, 2015, 2013, 2011; Tachibana et al, 2009). This effect was seemingly localized to the tuberal nucleus, when TN SST neurons were found to integrate into the melanocortin feeding system, though the effect of these neurons on feeding was attributed to γ-aminobutyric acid (GABA) release as opposed to direct SST effects (Luo et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Feeding Neurons Integrate Metabolic and Reproductive States in Mice

Massa

Scott

Cara

et al. 2023

Preprint

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Trade-offs between metabolic and reproductive processes are important for survival, particularly in mammals that gestate their young. Puberty and reproduction, as energetically taxing life stages, are often gated by metabolic availability in animals with ovaries. How the nervous system coordinates these trade-offs is an active area of study. We identify somatostatin neurons of the tuberal nucleus (TNSST) as a node of the feeding circuit that alters feeding in a manner sensitive to metabolic and reproductive states in mice. Whereas chemogenetic activation of TNSSTneurons increased food intake across sexes, selective ablation decreased food intake only in female mice during proestrus. Interestingly, this ablation effect was only apparent in animals with a low body mass. Fat transplantation and bioinformatics analysis of TNSSTneuronal transcriptomes revealed white adipose as a key modulator of the effects of TNSSTneurons on food intake. Together, these studies point to a mechanism whereby TNSSThypothalamic neurons modulate feeding by responding to varying levels of circulating estrogens differentially based on energy stores. This research provides insight into how neural circuits integrate reproductive and metabolic signals, and illustrates how gonadal steroid modulation of neuronal circuits can be context-dependent and gated by metabolic status.

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Chronic injection of pansomatostatin agonist ODT8-SST differentially modulates food intake and decreases body weight gain in lean and diet-induced obese rats

Cited by 5 publications

References 43 publications

Orexigenic response to tail pinch: role of brain NPY₁ and corticotropin releasing factor receptors

Orexigenic response to tail pinch: role of brain NPY₁ and corticotropin releasing factor receptors

High-protein diet selectively reduces fat mass and improves glucose tolerance in Western-type diet-induced obese rats

Feeding Neurons Integrate Metabolic and Reproductive States in Mice

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Chronic injection of pansomatostatin agonist ODT8-SST differentially modulates food intake and decreases body weight gain in lean and diet-induced obese rats

Cited by 5 publications

References 43 publications

Orexigenic response to tail pinch: role of brain NPY1 and corticotropin releasing factor receptors

Orexigenic response to tail pinch: role of brain NPY1 and corticotropin releasing factor receptors

High-protein diet selectively reduces fat mass and improves glucose tolerance in Western-type diet-induced obese rats

Feeding Neurons Integrate Metabolic and Reproductive States in Mice

Contact Info

Product

Resources

About

Orexigenic response to tail pinch: role of brain NPY₁ and corticotropin releasing factor receptors

Orexigenic response to tail pinch: role of brain NPY₁ and corticotropin releasing factor receptors