Chronic intermittent ethanol administration differentially alters DeltaFosB immunoreactivity in cortical-limbic structures of rats with high and low alcohol preference

Wscieklica, Tatiana; Sueur-Maluf, Luciana Le; Prearo, Leandro Campi; Conte, Rafael; Viana, Milena de Barros; Céspedes, Isabel Cristina

doi:10.1080/00952990.2019.1569667

Cited by 5 publications

(5 citation statements)

References 88 publications

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“…This discrepancy might be attributed to profound differences between these two unconditioned stimuli (ethanol and lithium). In contrast, in the present study, pERK expression was activated in the nuclei of the extended amygdala and in the basolateral amygdala, suggesting an overall complex and differential involvement of this kinase in brain areas involved in associative learning, reinforcement, and emotion (McDonald et al, 2010; Pati et al, 2019; Wscieklica et al, 2019).…”

Section: Discussioncontrasting

confidence: 99%

“…In particular, in animals receiving ethanol during conditioning under the schedule expected to elicit CPP, we found that the performance of the post-conditioning test resulted in a significant increase of pERK-positive neurons in the AcbC and AcbSh but not in the bed nucleus of stria terminalis, in the central nucleus of the amygdala and in the basolateral amygdala. On the contrary, in animals receiving ethanol during conditioning under the schedule expected to elicit CPA, the performance of the post-conditioning test resulted in a significant increase of pERK-positive neurons in the bed nucleus of stria terminalis, the central nucleus of the amygdala and the basolateral amygdala, suggesting a critical involvement of these areas (McDonald et al, 2010; Pati et al, 2019; Wscieklica et al, 2019), but not in the AcbC and AcbSh. These results, shown in Figures 6 and 7, indicate the conditioned stimulus, namely the environment associated with ethanol (forward conditioning, CPP) and that assigned to ethanol (backward conditioning, CPA), has a different impact on the phosphorylation of ERK in these brain areas.…”

Section: Discussionmentioning

confidence: 86%

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Effects of caffeine on ethanol-elicited place preference, place aversion and ERK phosphorylation in CD-1 mice

et al. 2020

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Background: Epidemiological studies indicate a rise in the combined consumption of caffeinated and alcoholic beverages, which can lead to increased risk of alcoholic-beverage overconsumption. However, the effects of the combination of caffeine and ethanol in animal models related to aspects of drug addiction are still underexplored. Aims: To characterize the pharmacological interaction between caffeine and ethanol and establish if caffeine can affect the ability of ethanol (2 g/kg) to elicit conditioned place preference and conditioned place aversion, we administered caffeine (3 or 15 mg/kg) to male CD-1 mice before saline or ethanol. Moreover, we determined if these doses of caffeine could affect ethanol (2 g/kg) elicited extracellular signal-regulated kinase phosphorylation in brain areas, nucleus accumbens, bed nucleus of stria terminalis, central nucleus of the amygdala, and basolateral amygdala, previously associated with this type of associative learning. Results: In the place-conditioning paradigm, caffeine did not have an effect on its own, whereas ethanol elicited significant conditioned-place preference and conditioned-place aversion. Caffeine (15 mg/kg) significantly prevented the acquisition of ethanol-elicited conditioned-place preference and, at both doses, also prevented the acquisition of ethanol-elicited conditioned-place aversion. Moreover, both doses of caffeine also prevented ethanol-elicited extracellular signal-regulated kinase phosphorylation expression in all brain areas examined. Conclusions: The present data indicate a functional antagonistic action of caffeine and ethanol on associative learning and extracellular signal-regulated kinase phosphorylation after an acute interaction. These results could provide exciting grounds for further studies, also in a translational perspective, of their pharmacological interaction modulating other processes involved in drug consumption and addiction.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 86%

Effects of caffeine on ethanol-elicited place preference, place aversion and ERK phosphorylation in CD-1 mice

et al. 2020

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“…The perfusion procedure was performed transcardially with saline 0.9% (100 ml) followed by a fixative solution containing 4% paraformaldehyde (500–700 ml) in an aqueous solution from paraformaldehyde heated to 60–65 °C (pH 9.5 at 4 °C) for 25 min ( Conte et al, 2019a , Conte et al, 2019b , Wscieklica et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

“…The samples were placed into heparinized tubes and centrifuged at 2300 rpm, 4 ºC for 15 min (Biochain, Newark, CA, USA). Blood alcohol concentration analysis was performed by the method of spectrophotometry with the enzyme kit for the enzyme NAD-ADH ( Conte et al, 2019a , Conte et al, 2019b , Wscieklica et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

Alcoholic neuropathy associated with chronic alcohol intake

Tessitore

Pereira-Rufino

Panfilio

et al. 2022

IBRO Neuroscience Reports

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“…Each animal, immediately after the behavioral test, was perfused in accordance with the protocol of Wscieklica et al (2019). This procedure was performed after the last session of alcohol exposure.…”

Section: Anesthesia Blood-analysis Perfusion and Microtomymentioning

confidence: 99%

Effects of moderate alcohol consumption on behavior and neural systems of Wistar rats

Conte

Zangirolame

Gobbo

et al. 2022

An. Acad. Bras. Ciênc.

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Chronic alcohol consumption affects various neurotransmitters, especially those implicated in the transitioning to alcohol use disorders (particularly dopaminergic and CRFergic systems). Few studies have investigated moderate alcohol consumption and its harmful consequences. The objective of this work was to analyze behavioral and neurochemical (dopaminergic and CRFergic systems) alterations during chronic moderate alcohol consumption. Twelve male Wistar rats were submitted to an intermittent alcohol ingestion protocol (alcohol group) for four weeks. The control group consisted of six rats. Open Field and Elevated Plus Maze tests were used for analysis of motor and anxietylike behaviors. Immunohistochemistry analysis was performed in dopaminergic and CRFergic systems. Animals exposed to alcohol consumed moderate doses, chronic and intermittently. Behavioral tests detected fewer fecal boli in the alcohol exposed group, and immunohistochemical analysis indicated fewer dopamine-immunoreactive cells in the ventral tegmental area, and more CRF-immunoreactive cells in the anterior cingulate cortex and dorsolateral septum in this group. Thus we concluded that Wistar rats that consumed moderate doses of alcohol voluntarily and chronically showed a discreet anxiolytic effect in behavior, and a hypodopaminergic and hyperCRFergic neurochemical condition, which together are strong inducers of alcohol consumption predisposing to the development of alcohol use disorder (AUD).

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Chronic intermittent ethanol administration differentially alters DeltaFosB immunoreactivity in cortical-limbic structures of rats with high and low alcohol preference

Cited by 5 publications

References 88 publications

Effects of caffeine on ethanol-elicited place preference, place aversion and ERK phosphorylation in CD-1 mice

Effects of caffeine on ethanol-elicited place preference, place aversion and ERK phosphorylation in CD-1 mice

Alcoholic neuropathy associated with chronic alcohol intake

Effects of moderate alcohol consumption on behavior and neural systems of Wistar rats

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