Chronic Kidney Disease is associated with an increase of Intimal Dendritic cells in a comparative autopsy study

Hueso, Miguel; Torras, Juan; Carrera, Marta; Vidal, August; Navarro, Estanis; Grinyó, Josep M.

doi:10.1186/s12950-015-0073-4

Cited by 11 publications

(9 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In people with early type 2 diabetes, arterial stiffness was associated positively with 18F‐fluorodeoxyglucose positron emission tomography‐assessed subclinical vascular inflammation . Studies performed recently showed that CKD is associated with an increase of DC in the intima of the abdominal aorta, pointing to an increased consumption of DC and thus DCP during vascular disease . This process might be considered as vascular inflammation, suggesting a role of DC in the pathogenesis of atherosclerotic vascular disease, especially in CKD .…”

Section: Discussionmentioning

confidence: 99%

Inflammation, vitamin D and dendritic cell precursors in chronic kidney disease

Paul

Franke

Nadal

et al. 2016

Clinical and Experimental Immunology

View full text Add to dashboard Cite

Decreased blood dendritic cell precursors (DCP) count is linked with atherosclerotic disease, while reduction of circulating DCP is also seen in patients with chronic kidney disease (CKD). As poor vitamin D status could be linked to a compromised innate immune response, we hypothesized that vitamin D status might be involved in the decrease in circulating DCP in CKD. Moreover, the potential role of inflammation was considered. Circulating myeloid (mDCP), plasmacytoid (pDCP) and total DCP (tDCP) were analysed using flow cytometry in 287 patients with CKD stage 3. Serum 25(OH)D and 1,25(OH)2D levels were measured using enzyme-linked immunosorbent assays (ELISA), interleukin (IL)-6, IL-10 and tumour necrosis factor (TNF)-α using cytometric bead array, C-reactive protein (CRP) using a high-sensitivity (hs) ELISA. Contrary to our hypothesis, there was no association between vitamin D levels and DCP, although their number was decreased significantly in CKD (P < 0·001). Instead, mDCP (r = -0·211) and tDCP (r = -0·188,) were associated slightly negatively with hsCRP but positively with the estimated glomerular filtration rate (eGFR, r = 0·314 for tDCP). According to multivariate linear regression, only higher hsCRP concentration and the presence of diabetes mellitus had a significant negative influence on DCP count (P < 0·03, respectively) but not vitamin D, age and eGFR. A significant impact of vitamin D on the reduction of circulating DCP in CKD 3 patients can be neglected. Instead, inflammation as a common phenomenon in CKD and diabetes mellitus had the main influence on the decrease in DCP. Thus, a potential role for DCP as a sensitive marker of inflammation and cardiovascular risk should be elucidated in future studies.

show abstract

Section: Discussionmentioning

confidence: 99%

Inflammation, vitamin D and dendritic cell precursors in chronic kidney disease

Paul

Franke

Nadal

et al. 2016

Clinical and Experimental Immunology

View full text Add to dashboard Cite

show abstract

“…The characteristics of patients and samples studied in this work have been reported previously [25,35]. Briefly, abdominal aortas were obtained from deceased patients from the HUB, and included atherosclerotic plaques, incipient atherosclerotic plaques, and normal abdominal aortas without injury.…”

Section: Methodsmentioning

confidence: 99%

An Exonic Switch Regulates Differential Accession of microRNAs to the Cd34 Transcript in Atherosclerosis Progression

Hueso

Cruzado

Navarro³

2019

Genes

Self Cite

View full text Add to dashboard Cite

Background: CD34+ Endothelial Progenitor Cells (EPCs) play an important role in the recovery of injured endothelium and contribute to atherosclerosis (ATH) pathogenesis. Previously we described a potential atherogenic role for miR-125 that we aimed to confirm in this work. Methods: Microarray hybridization, TaqMan Low Density Array (TLDA) cards, qPCR, and immunohistochemistry (IHC) were used to analyze expression of the miRNAs, proteins and transcripts here studied. Results: Here we have demonstrated an increase of resident CD34-positive cells in the aortic tissue of human and mice during ATH progression, as well as the presence of clusters of CD34-positive cells in the intima and adventitia of human ATH aortas. We introduce miR-351, which share the seed sequence with miR-125, as a potential effector of CD34. We show a splicing event at an internal/cryptic splice site at exon 8 of the murine Cd34 gene (exonic-switch) that would regulate the differential accession of miRNAs (including miR-125) to the coding region or to the 3’UTR of Cd34. Conclusions: We introduce new potential mediators of ATH progression (CD34 cell-clusters, miR-351), and propose a new mechanism of miRNA action, linked to a cryptic splicing site in the target-host gene, that would regulate the differential accession of miRNAs to their cognate binding sites.

show abstract

“…Of note, patients with CKD are known to show clinical and laboratory features of immunosuppression with low numbers of T-cells and circulating DCs as well as impaired T-cell activation [ 57 – 59 ], most likely as a consequence of suppressive effects of CKD on the bone marrow and cell maturation. Using immunohistochemistry, Hueso et al [ 60 ] investigated aortic lesions of CKD and control patients, i.e., adaptive as well as pathological intimal thickening of the aorta and fibroatheromas and found a significantly higher percentage of DCs in CKD patients compared to non-CKD controls. In contrast, in coronary arteries we found a lower number of DCs in CKD compared to nonrenal control patients.…”

Section: Role Of Systemic and Local Inflammation In Vascular Calcimentioning

confidence: 99%

Vascular Calcification in Chronic Kidney Disease: The Role of Inflammation

Benz

Hilgers

Daniel

et al. 2018

International Journal of Nephrology

View full text Add to dashboard Cite

Cardiovascular complications are extremely frequent in patients with chronic kidney disease (CKD) and death from cardiac causes is the most common cause of death in this particular population. Cardiovascular disease is approximately 3 times more frequent in patients with CKD than in other known cardiovascular risk groups and cardiovascular mortality is approximately 10-fold more frequent in patients on dialysis compared to the age- and sex-matched segments of the nonrenal population. Among other structural and functional factors advanced calcification of atherosclerotic plaques as well as of the arterial and venous media has been described as potentially relevant for this high cardiovascular morbidity and mortality. One potential explanation for this exceedingly high vascular calcification in animal models as well as in patients with CKD increased systemic and most importantly local (micro)inflammation that has been shown to favor the development of calcifying particles by multiple ways. Of note, local vascular upregulation of proinflammatory and proosteogenic molecules is already present at early stages of CKD and may thus be operative for vascular calcification. In addition, increased expression of costimulatory molecules and mast cells has also been documented in patients with CKD pointing to a more inflammatory and potentially less stable phenotype of coronary atherosclerotic plaques in CKD.

show abstract

Chronic Kidney Disease is associated with an increase of Intimal Dendritic cells in a comparative autopsy study

Cited by 11 publications

References 16 publications

Inflammation, vitamin D and dendritic cell precursors in chronic kidney disease

Inflammation, vitamin D and dendritic cell precursors in chronic kidney disease

An Exonic Switch Regulates Differential Accession of microRNAs to the Cd34 Transcript in Atherosclerosis Progression

Vascular Calcification in Chronic Kidney Disease: The Role of Inflammation

Contact Info

Product

Resources

About