Chronic lead exposure enhances the sympathoexcitatory response associated with P2X4 receptor in rat stellate ganglia

Zhu, Guo‐Qing; Chen, Zhenying; Dai, Bo; Zheng, Chaoran; Jiang, Huaide; Xu, Yurong; Xuan, Shi‐Ying; Guo, Jingjing; Yu, Dan; Liang, Shangdong; Li, Guilin

doi:10.1002/tox.22547

Cited by 13 publications

(12 citation statements)

References 54 publications

(75 reference statements)

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“…A detailed description of the potential mechanism of neurotoxicity is beyond the scope of this study. Experimental studies in animals suggested as potential mechanisms: stimulation of the sympathetic preganglionic neurons,10 harm to the β-adrenergic system in the brain,11 downregulation of cardiac β1-adrenoceptor activity,14 upregulated expression of P2X4 receptor in satellite glial cells of the stellate ganglion,15 increased sensitivity of chemoreceptor reflex12 or decreased sensitivity of the baroreflex 13. To what extent these animal experiments can be translated to the human condition remains unclear.…”

Section: Discussionmentioning

confidence: 99%

Heart rate variability and peripheral nerve conduction velocity in relation to blood lead in newly hired lead workers

Wei

Yang

et al. 2019

Occup Environ Med

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ObjectivesPrevious studies relating nervous activity to blood lead (BL) levels have limited relevance, because over time environmental and occupational exposure substantially dropped. We investigated the association of heart rate variability (HRV) and median nerve conduction velocity (NCV) with BL using the baseline measurements collected in the Study for Promotion of Health in Recycling Lead (NCT02243904).MethodsIn 328 newly hired men (mean age 28.3 years; participation rate 82.7%), we derived HRV measures (power expressed in normalised units (nu) in the high-frequency (HF) and low-frequency (LF) domains, and LF/HF) prior to long-term occupational lead exposure. Five-minute ECG recordings, obtained in the supine and standing positions, were analysed by Fourier transform or autoregressive modelling, using Cardiax software. Motor NCV was measured at the median nerve by a handheld device (Brevio Nerve Conduction Monitoring System, NeuMed, West Trenton, NJ, USA). BL was determined by inductively coupled plasma mass spectrometry.ResultsMean BL was 4.54 µg/dL (IQR 2.60–8.90 µg/dL). Mean supine and standing values of LF, HF and LF/HF were 50.5 and 21.1 nu and 2.63, and 59.7 and 10.9 nu and 6.31, respectively. Orthostatic stress decreased HF and increased LF (p<0.001). NCV averaged 3.74 m/s. Analyses across thirds of the BL distribution and multivariable-adjusted regression analyses failed to demonstrate any association of HRV or NCV with BL.ConclusionsAt the exposure levels observed in our study, autonomous nervous activity and NCV were not associated with BL.Trial registration numberNCT02243904

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Section: Discussionmentioning

confidence: 99%

Heart rate variability and peripheral nerve conduction velocity in relation to blood lead in newly hired lead workers

Wei

Yang

et al. 2019

Occup Environ Med

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“…8 According to their pharmacological characteristics and chemical structure, P receptors can be divided into two categories: P1 receptors, which respond to adenosine, and P2 receptors which respond to ATP. P2 (nucleotide) receptors can be further divided into two subcategories: P2X receptors (P2X 1−7 ) and P2Y receptors (P2Y 1,2,4,6,11,12,13,14,15 ). 8,9 In peripheral ganglia, the P2X4 receptor is expressed in both satellite glial cells and neurons.…”

Section: ■ Introductionmentioning

confidence: 99%

“…8,9 In peripheral ganglia, the P2X4 receptor is expressed in both satellite glial cells and neurons. 10,11 Injury and stimulation cause the nerve endings to release ATP, thereby initiating an inflammatory response. In sympathetic ganglia, ATP can be released from both glial cells and neurons 12−14 and activate P2X4 to participate in the transmission of pathological signals.…”

Section: ■ Introductionmentioning

confidence: 99%

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LncRNA UC.360+ shRNA Improves Diabetic Cardiac Sympathetic Dysfunction Mediated by the P2X4 Receptor in the Stellate Ganglion

Shi

Sun

Ren

et al. 2021

ACS Chem. Neurosci.

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Diabetic cardiac autonomic neuropathy (DCAN) is a complication that affects more than 60% of diabetic patients. There is evidence for the involvement of P2X4 receptor in DCAN. This study showed that the expression of the long noncoding RNA (lncRNA) UC.360+ was increased in the stellate ganglion (SG) of type 2 diabetes mellitus (DM) rats, and in situ hybridization revealed a clear presence of UC.360+ in SG neurons. The potential roles of UC.360+ in DCAN and its relationship with P2X4 receptor in SG were further explored via application of the short hairpin RNA (shRNA) against lncRNA UC.360+ in DM rats. The abnormal cardiac sympathetic changes in diabetic rats were improved after treatment with lncRNA UC.360+ shRNA. In the SG of these shRNA-treated DM rats, the upregulation of P2X4, tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β), and phosphorylated ERK1/2 was inhibited. Thus, lncRNA UC.360+ shRNA treatment may improve DCAN mediated by the P2X4 receptor in SG.

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“…GFAP was recently assessed to evaluate SGCs differentiation in cell culture condition [26].Collectively, GFAP was widely known as a marker of SGCs activation [27]. GS could catalyze adenosine triphosphate-dependent amidation of glutamate to glutamine and was known as a better marker for SGCs [28]. Tongtako et al found that SGCs in DRG were immunopositive for GFAP in dogs and monkeys, whereas GFAP in murine SGCs was absence.…”

Section: Introductionmentioning

confidence: 99%

Growth and Survival of Satellite Glial Cells in Different Culture Supplementations

Wei

Liu

Wang

et al. 2021

Preprint

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Background: In dorsal root ganglion (DRG), satellite glial cells (SGCs) tightly surrounded neurons and modulated microenvironment and sensory transmission. However, the biological properties of primary SGCs in culture were not fully understood. In the present study, we provided a method to harvest abundant and high-purity SGCs from neonatal rats. Three supplementations containing Dulbecco’s Modified Eagle Medium (DMEM)/F12, DMEM high glucose (HG) and Neurobasal-A (NB) were used to evaluate SGCs growth and survival in culture. Results: CCK-8 proliferation assay showed the increased proliferation in DMEM/F12 and DMEM/HG, but not in NB medium. NB medium caused cell death indicated by Bax, AnnexinV and PI staining. Glutamine was the major nutrition source for SGCs in culture and its exogenous application improved the poor proliferation and severe cell death in NB medium. SGCs markers GS and GFAP were similar in three supplementations and intensively expressed in culture. Differently, GS but not GFAP was remarkable in the intact DRG under normal condition. Conclusions: These results suggested that SGCs growth in culture depended on time and culture supplementation and DMEM/F12 medium was recommended to get high-purity SGCs. Glutamine was the major nutrition and the key molecule to maintain cell growth and survival in culture. Our study shed a new light on understanding the biological property and modulation of glial cells in the peripheral sensory ganglia.

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Chronic lead exposure enhances the sympathoexcitatory response associated with P2X4 receptor in rat stellate ganglia

Cited by 13 publications

References 54 publications

Heart rate variability and peripheral nerve conduction velocity in relation to blood lead in newly hired lead workers

Heart rate variability and peripheral nerve conduction velocity in relation to blood lead in newly hired lead workers

LncRNA UC.360+ shRNA Improves Diabetic Cardiac Sympathetic Dysfunction Mediated by the P2X4 Receptor in the Stellate Ganglion

Growth and Survival of Satellite Glial Cells in Different Culture Supplementations

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