Chronic multiscale imaging of neuronal activity in the awake common marmoset

Yamada, Yoshiyuki; Matsumoto, Yoshihisa; Okahara, Norio; Mikoshiba, Katsuhiko

doi:10.1038/srep35722

Cited by 27 publications

(17 citation statements)

References 27 publications

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“…NHPs can be used to study the neural circuits underlying social, cognitive, and motor behaviors that are highly relevant to humans. Two-photon microscopy, in combination with fluorescent, genetically encoded calcium indicators, has allowed the visualization of subcellular, single cellular, and ensemble neural dynamics and has become feasible in head-fixed monkeys (Nauhaus et al, 2012;Sadakane et al, 2015a;Seidemann et al, 2016;Yamada et al, 2016;Li et al, 2017;Ebina et al, 2018). However, complex behavior such as social interaction cannot be properly investigated using head fixation protocols.…”

Section: Introductionmentioning

confidence: 99%

Calcium Transient Dynamics of Neural Ensembles in the Primary Motor Cortex of Naturally Behaving Monkeys

et al. 2018

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To understand brain circuits of cognitive behaviors under natural conditions, we developed techniques for imaging neuronal activities from large neuronal populations in the deep layer cortex of the naturally behaving common marmoset. Animals retrieved food pellets or climbed ladders as a miniature fluorescence microscope monitored hundreds of calcium indicator-expressing cortical neurons in the right primary motor cortex. This technique, which can be adapted to other brain regions, can deepen our understanding of brain circuits by facilitating longitudinal population analyses of neuronal representation associated with cognitive naturalistic behaviors and their pathophysiological processes.

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Section: Introductionmentioning

confidence: 99%

Calcium Transient Dynamics of Neural Ensembles in the Primary Motor Cortex of Naturally Behaving Monkeys

et al. 2018

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“…Furthermore, having the capability to collect data from awake animals increases the proper translation of experimental conditions, as human subjects are studied in the conscious, awake condition. The use of conscious, awake NHPs in neuroimaging experiments is becoming increasingly popular, as exemplified by numerous studies ( Andersen et al, 2002 ; Belcher et al, 2016 ; Chen et al, 2012 ; Goense and Logothetis, 2008 ; Grinvald et al, 1991 ; Hori et al, 2020a ; Hung et al, 2015 ; Liu et al, 2013 ; Mantini et al, 2012b ; Nelissen and Vanduffel, 2011 ; Orban et al, 2004 ; Santisakultarm et al, 2016 ; Schaeffer et al, 2019 ; Sharma et al, 2019 ; Silva, 2017 ; Tootell et al, 2003 ; Tsukada et al, 2000 ; Vanduffel et al, 2001 ; Yamada et al, 2016 ). Much work has been done to compare functional data obtained in awake versus anesthetized conditions ( Barttfeld et al, 2015 ; Krzeminski et al, 2017 ; Liu et al, 2013 ; Liu et al, 2015a ; Uhrig et al, 2016 ; Uhrig et al, 2018 ).…”

Section: Non-invasive Imaging Methods Are Used In Experimental and CLmentioning

confidence: 99%

Using non-invasive neuroimaging to enhance the care, well-being and experimental outcomes of laboratory non-human primates (monkeys)

et al. 2021

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Over the past 10–20 years, neuroscience witnessed an explosion in the use of non-invasive imaging methods, particularly magnetic resonance imaging (MRI), to study brain structure and function. Simultaneously, with access to MRI in many research institutions, MRI has become an indispensable tool for researchers and veterinarians to guide improvements in surgical procedures and implants and thus, experimental as well as clinical outcomes, given that access to MRI also allows for improved diagnosis and monitoring for brain disease. As part of the PRIMEatE Data Exchange, we gathered expert scientists, veterinarians, and clinicians who treat humans, to provide an overview of the use of non-invasive imaging tools, primarily MRI, to enhance experimental and welfare outcomes for laboratory non-human primates engaged in neuroscientific experiments. We aimed to provide guidance for other researchers, scientists and veterinarians in the use of this powerful imaging technology as well as to foster a larger conversation and community of scientists and veterinarians with a shared goal of improving the well-being and experimental outcomes for laboratory animals.

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“…31 Many research groups have conducted multiscale brain imaging with cranial windows. [237][238][239] These methods are similar to the intravital windows used in QMIB research and could be applied to future studies. In summary, these selected examples have relevance to our current knowledge of breast cancer and could be potent tools for QMIB.…”

Section: Quantitative Multiscale Imaging Outside the Breastmentioning

confidence: 99%

Review of quantitative multiscale imaging of breast cancer

et al. 2018

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Breast cancer is the most common cancer among women worldwide and ranks second in terms of overall cancer deaths. One of the difficulties associated with treating breast cancer is that it is a heterogeneous disease with variations in benign and pathologic tissue composition, which contributes to disease development, progression, and treatment response. Many of these phenotypes are uncharacterized and their presence is difficult to detect, in part due to the sparsity of methods to correlate information between the cellular microscale and the whole-breast macroscale. Quantitative multiscale imaging of the breast is an emerging field concerned with the development of imaging technology that can characterize anatomic, functional, and molecular information across different resolutions and fields of view. It involves a diverse collection of imaging modalities, which touch large sections of the breast imaging research community. Prospective studies have shown promising results, but there are several challenges, ranging from basic physics and engineering to data processing and quantification, that must be met to bring the field to maturity. This paper presents some of the challenges that investigators face, reviews currently used multiscale imaging methods for preclinical imaging, and discusses the potential of these methods for clinical breast imaging.

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Chronic multiscale imaging of neuronal activity in the awake common marmoset

Cited by 27 publications

References 27 publications

Calcium Transient Dynamics of Neural Ensembles in the Primary Motor Cortex of Naturally Behaving Monkeys

Calcium Transient Dynamics of Neural Ensembles in the Primary Motor Cortex of Naturally Behaving Monkeys

Using non-invasive neuroimaging to enhance the care, well-being and experimental outcomes of laboratory non-human primates (monkeys)

Review of quantitative multiscale imaging of breast cancer

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