Chronic Ocular<i>Chlamydia trachomatis</i>Infection in Rabbits: Clinical and Histopathological Findings in the Posterior Segment

Boiko, Ernest V.; Pozniak, A L; Maltsev, Dmitrii S.; Суетов, А. А.; Nuralova, Irina V.

doi:10.1167/iovs.13-13416

Cited by 5 publications

(2 citation statements)

References 34 publications

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“…At present, China plays an important role of breeding rabbits, and domestic rabbits yield high quality meat and hair which are transported to many parts of home and abroad. However, disease is the main challenge for rabbit industry [ 11 , 12 ]. To date, there is little information about the seroprevalence of Chlamydia and the risk factors associated with Chlamydia infection in domestic rabbits in the world.…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence and Risk Factors ofChlamydiaInfection in Domestic Rabbits (Oryctolagus cuniculus) in China

Qin

Lou

et al. 2015

BioMed Research International

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Chlamydia spp. are obligate intracellular bacteria distributed all over the world, known to cause various forms of diseases in animals and humans. In the present study, a serological survey was conducted to detect the seroprevalence and risk factors associated with rabbit chlamydiosis in northeast China, including Liaoning province, Jilin province, Heilongjiang province, and Inner Mongolia Autonomous Region. Antibodies to Chlamydia were determined by indirect hemagglutination assay (IHA). The overall seroprevalence was estimated at 17.88% in total of 800 blood samples. The Chlamydia seroprevalence varied in domestic rabbits from different factors, and genders of domestic rabbits were considered as major risk factors associated with Chlamydia infection. Our study revealed a widespread and high prevalence of Chlamydia infection in domestic rabbits in northeast China, with higher exposure risk in female domestic rabbits. These findings suggested the potential importance of domestic rabbits in the transmission of zoonotic Chlamydia infection, and thus Chlamydia should be taken into consideration in diagnosing rabbit diseases. To our knowledge, there is no report of Chlamydia infection in domestic rabbits in China and the results extend the host range for Chlamydia, which has important implications for public health and the local economy.

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Section: Introductionmentioning

confidence: 99%

Seroprevalence and Risk Factors ofChlamydiaInfection in Domestic Rabbits (Oryctolagus cuniculus) in China

Qin

Lou

et al. 2015

BioMed Research International

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“…In our previous work, we have demonstrated infection of the RPE with the obligate intracellular human bacterial pathogen, Chlamydia trachomatis , following ocular inoculation in rabbits [ 12 ]. Yet, the possibility of infecting the hRPE with Chlamydia trachomatis and the pathogenetic significance of such an infectious process remain unknown.…”

Section: Introductionmentioning

confidence: 99%

Infection of Human Retinal Pigment Epithelium with Chlamydia trachomatis

et al. 2015

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PurposeLittle is known about the susceptibility of posterior segment tissues, particularly the human retinal pigment epithelium (hRPE), to Chlamydia trachomatis. The purpose of the study was to investigate the possibility of infecting the hRPE with Chlamydia trachomatis, and to examine the infectivity of different Chlamydia trachomatis clinical isolates for hRPE cells and the hRPE cell response to the infection.MethodsCultured hRPE and McCoy cells were inoculated with eight Chlamydia trachomatis (serovar E) clinical isolates at multiplicity of infection (MOI) of 2.0 or 0.3. To detect Chlamydia trachomatis, samples were stained immunohistochemically with anti-major outer membrane protein antibodies at 24h, 48h, and 72h postinoculation (PI). The changes in the expression of signaling molecules and proteins of cytoskeleton and extracellular matrix in hRPE cells were examined immunohistochemically.ResultsAll eight clinical isolates demonstrated ability to infect hRPE cells. At equal MOI of 0.3, the infectivity of Chlamydia trachomatis clinical isolates for RPE culture was found to be at least as high as that for McCoy cell culture. At 24h PI, the percentage of inclusion-containing cells varied from 1.5 ± 0.52 to 14.6 ± 3.3% in hRPE cell culture infected at MOI of 2.0 against 0.37 ± 0.34 to 8.9 ± 0.2% in McCoy cell culture infected at MOI of 0.3. Collagen type I, collagen type IV, basic fibroblast growth factor, transforming growth factor-beta and interleukin–8 expression at 48h PI were maximally increased, by 2.1-, 1.3-, 1.5-, 1.5- and 1.6-fold, respectively, in the Chlamydia trachomatis-infected compared with control hRPE cell culture specimens (P < 0.05).ConclusionsThis study, for the first time, proved the possibility of infecting hRPE cultured cells with Chlamydia trachomatis, which leads to proproliferative and proinflammatory changes in the expression of signaling molecules and extracellular matrix components.

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Targeting posterior eye infections with colloidal carriers: The case of Ganciclovir

Shah,

Patel

2023

International Journal of Pharmaceutics

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Chronic OcularChlamydia trachomatisInfection in Rabbits: Clinical and Histopathological Findings in the Posterior Segment

Cited by 5 publications

References 34 publications

Seroprevalence and Risk Factors ofChlamydiaInfection in Domestic Rabbits (Oryctolagus cuniculus) in China

Seroprevalence and Risk Factors ofChlamydiaInfection in Domestic Rabbits (Oryctolagus cuniculus) in China

Infection of Human Retinal Pigment Epithelium with Chlamydia trachomatis

Targeting posterior eye infections with colloidal carriers: The case of Ganciclovir

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