Chronic rhinosinusitis with nasal polyposis (CRSwNP): the correlation between expression of Galectin-10 and Clinical-Cytological Grading (CCG)

Abstract

Background Chronic rhinosinusitis with nasal polyps (CRSwNP) is typically characterized by Type 2 inflammation. Several biomarkers of eosinophilic inflammation, including Galectin-10, also known as Charcot-Leyden crystal protein (CLCP), have been identified to establish eosinophilic infiltration of polyps, a reliable predictor of recurrence. Objective: We aimed to evaluate the Galectin-10 expression in nasal polyps of patients with CRSwNP and to assess the correlation of Charcot-Leyden crystals expression to the severity of CRSwNP according to Clinical-Cytological Grading (CCG). Methods A double-label immunofluorescence was performed to evaluate the expression of Gal-10, CD15, Tryptase, and CD63 and their eventual co-localization on histological samples of 18 patients with CRSwNP. Double-positive Gal-10+CD15+ and Galectin-10+Tryptase+ inflammatory cells were counted by confocal microscopy. Results Galectin-10 was detectable in all examined tissues from CRSwNP patients, and its expression increased as low, medium and high CCG tissues were examined, respectively. Galectin-10 was extensively present in inflammatory cells, while limited Galectin-10 deposits were detected around mucosal epithelial cells. Conclusion We showed the strong correlation between CCG and Galectin-10 expression, mainly colocalized with infiltrating eosinophils and mast-cells, in patients affected by CRSwNP.

Association between Endometrial Polyps and Chronic Endometritis: Is It Time for a Paradigm Shift in the Pathophysiology of Endometrial Polyps in Pre-Menopausal Women? Results of a Systematic Review and Meta-Analysis

“…Chronic inflammation has been identified as a causative factor in the development of polyps in different mucosal tissues across the human body (i.e., urinary tract, upper respiratory tract and lower gastrointestinal tract) [ 47 , 48 , 49 ]. With regard to EPs, a potential inflammation-driven pathogenesis has been long neglected by the scientific community.…”

Quantification of Aspergillus fumigatus antigen Asp f 1 in airway tissue and allergic inflammation

“…9 Although tissue-infiltrating eosinophil count does not always correlate with disease activity or severity, 10 the significant correlation between galectin-10 expression and clinicalcytological grading in NPs the of patients with CRSwNP has been reported. 11 We observed galectin-10 levels and tissue eosinophil counts in the NPs. Galectin-10 is a cytoplasmic protein of eosinophils released from cytolytic cells 12 and has potential as a biomarker for activated eosinophils in eosinophilic inflammatory diseases.…”

Lactose and Galactose Promote the Crystallization of Human Galectin-10

“…Although Charcot–Leyden crystal proteins are not a major determinant of human regulatory T-cell function (viability) [ 41 ], CLC is one of luminal-captured biomarkers of inflammation and eosinophilic esophagitis [ 42 ], and Gal-10 expression in the nasal polyps of patients with chronic rhinosinusitis was correlated with the severity of the disease according to Clinical-Cytological Grading [ 43 ]. Logistic regression analysis showed that crystalline CLCs in nasal tissues are predictive of nasal polyp recurrence.…”