Chronic Trypanosoma cruzi infection in the rat: Cyclophosphamide-induced recovery of adjuvant arthritis correlates with changes in the levels of lymph node T-lymphocytes and class II+ cells

Didoli, Griselda; Revelli, Silvia; Davila, Hector; Ferro, Maria Elena; Romero-Piffiguer, Marta

doi:10.1016/0192-0561(95)00117-4

Cited by 1 publication

(5 citation statements)

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“…Data from day 15 postinduction showed that control-Mo and Tc-Mo rats had a significantly lower arthritic score when compared to controls (P<0.001; Figure 1). This represented a 40-45% reduction in arthritis severity, and was consistent with published data (8)(9)(10). As shown in the same Figure, offspring born to mothers receiving IFN-g during gestation (TcIFN-Mo and IFNMo groups) presented a reestablished arthritic response which remained within the range observed in controls.…”

Section: Resultssupporting

confidence: 79%

“…Consistent with this reasoning are data from offspring born to IFN-g mothers in which restored AA coincided with a reestablished balance of major T cell subsets. Additional support for the view that T cells populating the regional LN are critical for the establishment of arthritic lesions comes from our immunolabelling cell studies in LN from chronically T. cruzi-infected (10). Cyinduced recovery of AA was accompanied by a restored relationship between CD4 + and CD8 + cells.…”

Section: Discussionmentioning

confidence: 67%

“…In addition to protecting offspring from acute T. cruzi infection (14), the present observations indicate that administration of IFN-g to pregnant rats also reversed the immunodepressed responses occurring during the chronic period of this experimental infection (8)(9)(10). In fact, offspring born to mothers receiving IFN-g during gestation (TcIFN- Mo and IFN-Mo groups) not only presented a recovered arthritic response but also displayed substantial modifications in the levels of major T lymphocyte populations.…”

Section: Discussionmentioning

confidence: 81%

“…Microscopy studies have revealed that affected joints are heavily infiltrated by lymphocytes, macrophages, epithelioid and giant multinucleate cells resembling a chronic granulomatous reaction (16). The arthritis was evaluated at 7, 15, 20 and 30 days postinduction by direct examination of individual joints using a semiquantitative scale (10). The arthritis scores were calculated as follows: smaller joints (metatarsophalangeal and interphalangeal), 1 point; larger joints (tarsus and ankle), 2 points.…”

Section: Induction and Assessment Of Adjuvant-induced Arthritismentioning

confidence: 99%

“…Accompanying these abnormalities, immunolabelling studies have shown that spleen and lymph nodes (LN) from chronically infected rats display an inverse CD4/CD8 T cell ratio in the presence of increased levels of mononuclear cells expressing class II major histocompatibility (MHC) antigens (4,10).…”

Section: Introductionmentioning

confidence: 99%

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Administration of interferon-<FONT FACE=Symbol>g</font> to pregnant rats reverses the depressed adjuvant-induced arthritis of their chronically Trypanosoma cruzi-infected offspring

Didoli

Revelli

Davila

et al. 1999

Braz J Med Biol Res

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We demonstrated that administration of interferon gamma (IFN-g) to the inbred l strain of pregnant rats conferred partial resistance on their offspring to challenge with Trypanosoma cruzi. We now examine if this intervention also modifies the reportedly immunodepressed cellular responses which occur during chronic infection. Offspring were born to mothers undergoing one of the following procedures during gestation: subcutaneous injections of recombinant rat IFN-g, 50,000 IU/rat, five times/week for 3 weeks, which was started on the day of mating (IFN-Mo); infection with 10 6 trypomastigotes of T. cruzi at 7, 14, and 21 days after mating plus IFN-g treatment as given to the former group (TcIFN-Mo); the same protocol except that physiological saline was injected instead of IFN-g (Tc-Mo); injection of physiological saline only (control-Mo). All offspring groups (N = 8-10/group) were infected at weaning and were assessed 90 days later for their adjuvant-induced arthritic response or levels of major T cell subsets in spleen and lymph nodes. TcIFN-Mo and IFN-Mo offspring showed a reestablished arthritic response, which remained within the range seen in controls. Immunolabeling studies on parallel groups of 90-day-infected offspring showed that the inverse CD4/CD8 cell ratio that is usually seen in lymphoid organs from these chronically infected rats (median 0.61) appeared to have recovered in the TcIFN-Mo and IFN-Mo groups (median 1.66 and 1.78, respectively) and was not different from uninfected controls (1.96). These studies indicate that early stimulation with IFN-g is able to reverse the immunosuppressive state that is usually present during the chronic period of the experimental infection.

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Section: Resultssupporting

confidence: 79%

Section: Discussionmentioning

confidence: 67%