Chronic unpredictable mild stress alters an anxiety-related defensive response, Fos immunoreactivity and hippocampal adult neurogenesis

Andrade, Januário de; Céspedes, Isabel Cristina; Abrão, Renata Oliveira; Santos, Thays Brenner; Diniz, L.; Britto, Luiz R.G.; Spadari-Bratfisch, Regina Célia; Ortolani, Daniela; Melo-Thomas, Liana; Silva, R.C.B. da; Viana, Milena de Barros

doi:10.1016/j.bbr.2013.04.031

Cited by 61 publications

(40 citation statements)

References 64 publications

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“…Compared to wildtype littermates, CRH −/− mutants showed increased SVZ proliferation (p<0.05, 4942 ± 302 cells in CRH −/− , compared to 3906 ± 354 cells in CRH +/+ mice), but decreased cell survival in the OB (p<0.005, 25 ± 1 cell in CRH −/− , compared to 36 ± 2 cells in CRH +/+ mice) (Figure 4B and C). This increased proliferation in CRH −/− mice is consistent with previous reports that stress impairs neurogenesis both in the SVZ and in the hippocampus (de Andrade et al, 2013; Hitoshi et al, 2007, Schoenfeld and Gould, 2013), and that CRH −/− mice show decreased stress levels (Jacobson et al, 2000). Cleaved caspase-3 and TUNEL staining revealed increased apoptosis in the GCL of CRH −/− mice (Figure S4A).…”

Section: Resultssupporting

confidence: 92%

Local CRH Signaling Promotes Synaptogenesis and Circuit Integration of Adult-Born Neurons

et al. 2014

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Summary Neural activity either enhances or impairs de novo synaptogenesis and circuit integration of neurons, but how this activity is mechanistically relayed in the adult brain is largely unknown. Neuropeptide-expressing interneurons are widespread throughout the brain and are key candidates for conveying neural activity downstream via neuromodulatory pathways that are distinct from classical neurotransmission. With the goal of identifying signaling mechanisms that underlie neuronal circuit integration in the adult brain, we have virally traced local Corticotropin-releasing hormone (CRH)-expressing inhibitory interneurons with extensive presynaptic inputs onto new neurons that are continuously integrated into the adult rodent olfactory bulb. Local CRH signaling onto adult-born neurons promotes and/or stabilizes chemical synapses in the olfactory bulb, revealing a neuromodulatory mechanism for continued circuit plasticity, synapse formation, and integration of new neurons in the adult brain.

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Section: Resultssupporting

confidence: 92%

Local CRH Signaling Promotes Synaptogenesis and Circuit Integration of Adult-Born Neurons

et al. 2014

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“…Supporting this, Santarelli and colleagues [76] found that antidepressant drugs failed to exert positive behavioral outcomes in tests of emotionality when their neurogenic effects on the hippocampus were blocked. Additionally, chronic stress decreases neurogenesis, increases emotional reactivity, and is a potent contributing factor in mood disorders [77,78]. We provide further support to these patterns: in the present study, those diet manipulations with the biggest positive effects on emotional behaviors also significantly increased hippocampal neurogenesis.…”

Section: Discussionsupporting

confidence: 85%

Adult emotionality and neural plasticity as a function of adolescent nutrient supplementation in male rats

McCall

Mahadevia

Corriveau

et al. 2015

Pharmacology Biochemistry and Behavior

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The present study explored the effects of supplementing male rats with either choline, omega-3 fatty acids, or phytoestrogens, from weaning into early adulthood, on emotionality and hippocampal plasticity. Because of the neuroprotective properties of these nutrients, we hypothesized that they would positively affect both behavior and hippocampal function when compared to non-supplemented control rats. To test this hypothesis, male Sprague Dawley rats were assigned to one of four nutrient conditions after weaning: 1) control (normal rat chow); 2) choline (supplemented in drinking water); 3) omega 3 fatty acids (daily oral supplements); or 4) phytoestrogens (supplemented in chow). After 4 weeks on their respective diets, a subset of rats began 3 weeks of behavioral testing, while the remaining behaviorally naïve rats were sacrificed after 6 weeks on the diets to assess numbers of adult-born hippocampal neurons using the immature neuron marker, doublecortin. The results revealed that choline supplementation affected emotional functioning; compared to rats in other diet conditions, rats in this group were less anxious in an open field and after exposure to predator odor and showed less behavioral despair after forced swimming. Similar behavioral findings were evident following supplementation with omega-3 fatty acids and phytoestrogens supplementation, though not on all tests and not to the same magnitude. Histological findings followed a pattern consistent with the behavioral findings: choline supplementation, followed by omega-3 fatty acid supplementation, but not phytoestrogen supplementation, significantly increased the numbers of new-born hippocampal neurons. Choline and omega −3 fatty acids have similar biological functions—affecting cell membranes, growth factor levels, and epigenetically altering gene transcription. Thus, the present findings suggest that targeting nutrients with these effects may be a viable strategy to combat adult psychopathologies.

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“…Brains were removed and postfixed overnight in PFA at 4 • C, placed in 30% sucrose until they sank, then frozen on dry ice and sectioned at 50 m using a cryostat. All sections containing the subventricular zone (SVZ -+1.10 to +0.02 mm from Bregma), and subgranular zones (SGZ) of the dorsal hippocampus (dHipp) and ventral hippocampus (vHipp) (−1.8 to −5.2 mm and −5.2 to −6.7 mm from Bregma, respectively -as indicated in de Andrade et al [32]) were collected so as to obtain three sets of each region. Sections were stored in cryoprotectant solution at −20 • C until immunohistochemistry on free-floating sections was performed.…”

Section: Immunohistochemistry For Doublecortin (Dcx) Expressionmentioning

confidence: 99%

Npas4 deficiency increases vulnerability to juvenile stress in mice

Coutellier

Gilbert

Shepard

2015

Behavioural Brain Research

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Chronic unpredictable mild stress alters an anxiety-related defensive response, Fos immunoreactivity and hippocampal adult neurogenesis

Cited by 61 publications

References 64 publications

Local CRH Signaling Promotes Synaptogenesis and Circuit Integration of Adult-Born Neurons

Local CRH Signaling Promotes Synaptogenesis and Circuit Integration of Adult-Born Neurons

Adult emotionality and neural plasticity as a function of adolescent nutrient supplementation in male rats

Npas4 deficiency increases vulnerability to juvenile stress in mice

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