Chronic Upper Airway Obstruction Induces Abnormal Sleep/Wake Dynamics in Juvenile Rats

Gradwohl, Gérard; Berdugo-Boura, Nilly; Segev, Yael; Tarasiuk, Ariel

doi:10.1371/journal.pone.0097111

Cited by 6 publications

(15 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sleep was recorded 7 days following recovery from surgery when the animals had acclimatized to the recording environment. Raw EEG and EMG outputs from the skull and skeletal muscle electrodes were sampled at 250 Hz, filtered at 0.5–40 Hz and 10–250 Hz, respectively, using DSI system [ 4 , 5 , 28 ].…”

Section: Methodsmentioning

confidence: 99%

“…The speed of the spectral change was calculated as the distance between two consecutive data points (calculated from the horizontal and vertical velocities by the Pythagorean Theorem), and in this sense, the temporal resolution was second by second. After this calculation, we mapped the velocities to a 1000 × 1000 grid with the values at each point representing the average velocities originating at that site [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

“…Trajectories connecting different clusters were considered to be valid transitions if: 1) the duration of the trajectory connecting two different clusters was 60 seconds and 2) the trajectory spent at least half of the preceding 30 seconds in the initiating cluster and half of the 30 subsequent seconds in the terminating cluster. In addition, we explored the micro-arousal change due to maturation during 24 hours [ 28 , 31 ].…”

Section: Methodsmentioning

confidence: 99%

“…The traditional scoring methods simply identify discrete states at a time window (4 to 10 seconds), so they can overlook important variations within states, or often can exclude or dilute events through averaging; and therefore rapid changes between these states or within a specific state may be disregarded. The 2-dimensional (2D) state space technique (SST) provides a novel unbiased approach, a non-categorical method for comparison and quantitative analysis of sleep/wake states [ 28 – 30 ]. The previous application of SST of sleep recordings used local field potential data, but the variability in these signals prevented comparisons between animals [ 31 – 33 ].…”

Section: Introductionmentioning

confidence: 99%

“…The previous application of SST of sleep recordings used local field potential data, but the variability in these signals prevented comparisons between animals [ 31 – 33 ]. Recently the SST was further developed to enable inter-animal comparisons of EEG dynamics of sleep/wake behavior [ 28 – 30 ]. SST has high temporal resolution and analyzes behavior as a continuum, rather than discrete states, thus facilitating higher dimensional examination of state transitions that are not possible with traditional methods.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Sleep/Wake Dynamics Changes during Maturation in Rats

et al. 2015

Self Cite

View full text Add to dashboard Cite

ObjectivesConventional scoring of sleep provides little information about the process of transitioning between vigilance states. We applied the state space technique (SST) using frequency band ratios to follow normal maturation of different sleep/wake states, velocities of movements, and transitions between states of juvenile (postnatal day 34, P34) and young adult rats (P71).Design24-h sleep recordings of eight P34 and nine P71 were analyzed using conventional scoring criteria and SST one week following implantation of telemetric transmitter. SST is a non-categorical approach that allows novel quantitative and unbiased examination of vigilance-states dynamics and state transitions. In this approach, behavioral changes are described in a 2-dimensional state space that is derived from spectral characteristics of the electroencephalography.Measurements and ResultsWith maturation sleep intensity declines, the duration of deep slow wave sleep (DSWS) and light slow wave sleep (LSWS) decreases and increases, respectively. Vigilance state determination, as a function of frequency, is not constant; there is a substantial shift to higher ratio 1 in all vigilance states except DSWS. Deep slow wave sleep decreases in adult relative to juvenile animals at all frequencies. P71 animals have 400% more trajectories from Wake to LSWS (p = 0.005) and vice versa (p = 0.005), and 100% more micro-arousals (p = 0.021), while trajectories from LSWS to DSWS (p = 0.047) and vice versa (p = 0.033) were reduced by 60%. In both juvenile and adult animals, no significant changes were found in sleep velocity at all regions of the 2-dimensional state space plot; suggesting that maturation has a partial effect on sleep stability.ConclusionsHere, we present novel and original evidence that SST enables visualization of vigilance-state intensity, transitions, and velocities that were not evident by traditional scoring methods. These observations provide new perspectives in sleep state dynamics and highlight the usefulness of this technique in exploring the development of sleep-wake activity.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Sleep/Wake Dynamics Changes during Maturation in Rats

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

Abnormal Growth and Feeding Behavior Persist After Removal of Upper Airway Obstruction in Juvenile Rats

Assadi

Shknevsky

Segev

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

Pediatric obstructive sleep-disordered breathing is associated with growth retardation, but also with obesity that has a tendency to persist following treatment. We investigated the effect of upper airways obstruction (AO) and of obstruction removal (OR) in juvenile rats on gut-derived ghrelin and related hypothalamic factors, feeding, and growth hormone (GH) homeostasis. Here, we show that after seven weeks of AO, animals gained less weight compared to controls, despite an increase in food intake due to elevated ghrelin and hypothalamic feeding factors. OR rats who had complete restoration of tracheal diameter, consumed more food due to increased ghrelin and exhibited growth retardation due to deregulation of GH homeostasis. This study is the first to show dysregulation of the hormonal axes controlling feeding behavior and growth that are not fully restored following OR. Thus, surgical treatment by itself may not be sufficient to prevent post-surgical increased food intake and growth retardation.

show abstract

Irreversible metabolic abnormalities following chronic upper airway loading

Assadi

Segev

Tarasiuk

2019

Sleep

View full text Add to dashboard Cite

Study Objectives Treatment of obstructive sleep apnea increases obesity risk by an unclear mechanism. Here, we explored the effects of upper airway obstruction and its removal on respiratory homeostasis, energy expenditure, and feeding hormones during the sleep/wake cycle from weaning to adulthood. Methods The tracheas of 22-day-old rats were narrowed, and obstruction removal was performed on post-surgery day 14. Energy expenditure, ventilation, and hormone-regulated feeding were analyzed during 49 days before and after obstruction. Results Energy expenditure increased and body temperature decreased in upper airway obstruction and was only partially recovered in obstruction removal despite normalization of airway resistance. Increased energy expenditure was associated with upregulation of ventilation. Decreased body temperature was associated with decreased brown adipose tissue uncoupling protein 1 level, suppressed energy expenditure response to norepinephrine, and decreased leptin level. Upper airway obstructed animals added less body weight, in spite of an increase in food intake, due to elevated hypothalamic orexin and neuropeptide Y and plasma ghrelin. Animals who underwent obstruction removal fed more due to an increase in hypothalamic neuropeptide Y and plasma ghrelin. Conclusions The need to maintain respiratory homeostasis is associated with persistent abnormal energy metabolism and hormonal regulation of feeding. Surgical treatment per se may not be sufficient to correct energy homeostasis, and endocrine regulation of feeding may have a larger effect on weight change.

show abstract

Chronic Upper Airway Obstruction Induces Abnormal Sleep/Wake Dynamics in Juvenile Rats

Cited by 6 publications

References 51 publications

Sleep/Wake Dynamics Changes during Maturation in Rats

Sleep/Wake Dynamics Changes during Maturation in Rats

Abnormal Growth and Feeding Behavior Persist After Removal of Upper Airway Obstruction in Juvenile Rats

Irreversible metabolic abnormalities following chronic upper airway loading

Contact Info

Product

Resources

About