Chronic valproate treatment influences folliculogenesis and reproductive hormones with possible ameliorating role for folic acid in adult albino rats

Ibrahim, Ibrahim Hassan; Aboregela, Adel Mohamed; Gouda, Rehab Hassan Elbanna; Eid, Khaled Adel

doi:10.1016/j.acthis.2019.07.003

Cited by 8 publications

(9 citation statements)

References 34 publications

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“…In concordance with our observations, a work Gregoraszczuk et al concluded that VPA decreased the release of estradiol and progesterone from follicular cells isolated from small-and medium-sized porcine ovarian follicles [52] . In support to our results, Ibrahim et al found a significant decline in serum reproductive hormones concentrations (estradiol, progesterone, FSH and LH) in a rat model of VPA-induced ovarian failure compared with control group along with a marked elevation of their concentrations following FA supplementation [30] . Contradictory to our findings, a study of Røste et al showed no significant alteration in serum progesterone and FSH in VPA-exposed rats in comparison with controls [37] .…”

Section: Discussionsupporting

confidence: 92%

“…It is well recognized that VPA has detrimental consequences on reproductive function in both sexes in human beings [3,30,37] . Ovarian failure is defined by steroid deficiency, ovulation defect, and atretic follicles increase.…”

Section: Discussionmentioning

confidence: 99%

“…All treatments were administrated for 90 successive days. Doses of VPA, FA and PRP were selected based on earlier studies concerned with ovarian toxicities [20,[28][29][30][31] .…”

Section: Experimental Protocolmentioning

confidence: 99%

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Do platelet rich plasma and folic acid reverse experimental induced ovarian failure: Emphasis on folliculogenesis, and ovarian steroidogenesis

Faruk

2021

Egyptian Journal of Histology

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Background: Valproic acid (VPA) is a commonly prescribed antiepileptic drug. VPA could mediate endocrinal reproductive dysfunction on a long-term use. This study was designed to evaluate the possible protective effects of platelet rich plasma (PRP) alone and PRP in combination with folic acid (FA) in a rat model of VPA induced ovarian failure. Materials and Methods: Thirty-five adult rats were randomly divided into five equal groups and 14 female rats were used for PRP preparation as control, VPA, co-treated VPA with FA, co-treated VPA with PRP and co-treated VPA with FA and PRP groups. All treatments were administered for 90 days. The effects of PRP and/or FA against VPA were evaluated through assessment of ovarian oxidant/antioxidant biomarkers, hormonal assay of reproductive hormones, quantification of mRNA gene expression for ovarian steroidogenesis pathway-encoding genes. Histopathological examination of the ovarian tissues was implemented. Results: Revealed that VPA exposure caused ovarian failure as documented by the decreased ovarian superoxide dismutase and catalase activities, increased ovarian malondialdehyde levels, diminished serum reproductive hormones concentrations and repressed the ovarian steroidogenesis pathway-encoding genes. In addition, VPA-induced marked ovarian histopathological alterations and impaired folliculogenesis. PRP and/or FA treatment improved ovarian function after VPA exposure. Adding FA to PRP produced more ovarian estrogen receptors immunoexpression than those produced by PRP alone. While other protective effects against VPA induced ovarian failure are equal between the two agents. Conclusion: PRP exhibited a protective role against VPA-induced ovarian failure in adult rats. A combined PRP and FA therapy is superior to PRP alone to some extent.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

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Do platelet rich plasma and folic acid reverse experimental induced ovarian failure: Emphasis on folliculogenesis, and ovarian steroidogenesis

Faruk

2021

Egyptian Journal of Histology

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“…Several studies have also verified that PCO formation, increased LH levels, or increased LH/FSH ratio and menstrual disorders are commonly seen in patients treated with VPA (12,16,72,73). Furthermore, an animal experiment showed that, compared with those of the control group, the quantity of follicles was significantly reduced, the atretic follicles were increased, and the ovary also showed multiple cystic follicles in the VPA group (109). The reported rate of polycystic ovaries in WWE is as high as 40% (110).…”

Section: The Possible Mechanism Of Ovulation Disorder and Polycystic Ovary Induced By Vpamentioning

confidence: 86%

“…The high androgen level in patients also affects the development and maturation of follicles (111). In addition, after treatment with VPA, the secretion of TGF-b1 in the follicle decreases (109), and the TGF-b superfamily exerts an indispensable effect in regulating the formation and development of follicles (112). In addition, some scholars proposed that VPA can participate in the process of apoptosis of ovarian cells by enhancing the level of the apoptotic hormone testosterone and activating the caspase-3-dependent apoptosis signaling pathway (100).…”

Section: The Possible Mechanism Of Ovulation Disorder and Polycystic Ovary Induced By Vpamentioning

confidence: 99%

Research Progress on the Effect of Epilepsy and Antiseizure Medications on PCOS Through HPO Axis

Zhang

Wei

et al. 2021

Front. Endocrinol.

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Epilepsy is a common chronic neurological disease that manifests as recurrent seizures. The incidence and prevalence of epilepsy in women are slightly lower than those in men. Polycystic ovary syndrome (PCOS), a reproductive endocrine system disease, is a complication that women with epilepsy are susceptible to, and its total prevalence is 8%–13% in the female population and sometimes as high as 26% in female epilepsy patients. The rate of PCOS increased markedly in female patients who chose valproate (VPA), to 1.95 times higher than that of other drugs. In addition, patients receiving other anti-seizure medications (ASMs), such as lamotrigine (LTG), oxcarbazepine (OXC), and carbamazepine (CBZ), also have reproductive endocrine abnormalities. Some scholars believe that the increase in incidence is related not only to epilepsy itself but also to ASMs. Epileptiform discharges can affect the activity of the pulse generator and then interfere with the reproductive endocrine system by breaking the balance of the hypothalamic–pituitary–ovarian (HPO) axis. ASMs may also cause PCOS-like disorders of the reproductive endocrine system through the HPO axis. Moreover, other factors such as hormone metabolism and related signalling pathways also play a role in it.

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Bisphenol A enhances apoptosis, fibrosis, and biochemical fluctuations in the liver of adult male rats with possible regression after recovery

Amin

Sonpol

Gouda

et al. 2022

The Anatomical Record

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Bisphenol A (BPA) is an environmental contaminant that might be harmful. Human exposure to BPA can occur during the fetal and postnatal periods and extends throughout life. This study aimed to estimate the effects of oral administration of BPA on rat liver and assess the possibility of recovery after cessation. Adult male albino rats were orally administered with BPA (50 mg/kg body weight) for 8 weeks, and then one group was left to recover for 4 weeks. Histological, immunohistochemical, biochemical, and quantitative real‐time polymerase chain reaction assessments were performed. Loss of hepatic architecture, vascular dilatation congestion, and exudation, as well as cellular vacuolation, fat accumulation, and pyknotic nuclei were detected. Furthermore, inflammatory infiltration, localized metaplasia, and excessive collagen deposition in the portal triad were observed. Expression of Bcl‐2‐associated X protein and transforming growth factor beta 1 was prominent, denoting apoptosis and fibrosis. After the administration of BPA, serum levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, cholesterol, triglycerides, and low‐density lipoproteins were enhanced. Additionally, total protein, albumin, and high‐density lipoproteins decreased. After a recovery for 4 weeks, hepatic cellular and vascular pathologies returned to normal, except for some inflammatory infiltration. Regarding biochemical affection, most of the parameters were directed toward normal during recovery. However, most of them were still significantly different from controls. This explored BPA hepatotoxicity from structural and functional aspects, and the possible spontaneous reversibility was confirmed. However, the precise mechanisms underlying hepatotoxicity or recovery need more in‐depth investigations.

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Chronic valproate treatment influences folliculogenesis and reproductive hormones with possible ameliorating role for folic acid in adult albino rats

Cited by 8 publications

References 34 publications

Do platelet rich plasma and folic acid reverse experimental induced ovarian failure: Emphasis on folliculogenesis, and ovarian steroidogenesis

Do platelet rich plasma and folic acid reverse experimental induced ovarian failure: Emphasis on folliculogenesis, and ovarian steroidogenesis

Research Progress on the Effect of Epilepsy and Antiseizure Medications on PCOS Through HPO Axis

Bisphenol A enhances apoptosis, fibrosis, and biochemical fluctuations in the liver of adult male rats with possible regression after recovery

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