Chronological Changes in the Ultrastructure of Titanium‐Bone Interfaces: Analysis by Light Microscopy, Transmission Electron Microscopy, and Micro‐Computed Tomography

Morinaga, Kenzo; Kakemoto, Hirofumi; Sato, Atsuko; Watazu, Akira; Matsuura, Minoru

doi:10.1111/j.1708-8208.2008.00093.x

Cited by 32 publications

(30 citation statements)

References 30 publications

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“…Similar observations have been reported in several different TiO 2 dental implant surfaces in in vitro and in vivo rat tibia studies. 11,[35][36][37][38] The proposed mechanism of titanium-associated osteoinduction is thought to be related to hydroxyl groups and calcium ions present in TiO 2 surfaces. These hydroxyl groups may well promote adsorption of calcium-binding extracellular matrix proteins (e.g., alkaline phosphatase) and RGD-specific peptide sequences (e.g., fibronectin, bone sialoprotein).…”

Section: Discussionmentioning

confidence: 99%

Titanium-Enriched Hydroxyapatite–Gelatin Scaffolds with Osteogenically Differentiated Progenitor Cell Aggregates for Calvaria Bone Regeneration

Ferreira

Padilla

Urkasemsin

et al. 2013

Tissue Engineering Part A

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Adequate bony support is the key to re-establish both function and esthetics in the craniofacial region. Autologous bone grafting has been the gold standard for regeneration of problematic large bone defects. However, poor graft availability and donor-site complications have led to alternative bone tissue-engineering approaches combining osteoinductive biomaterials and three-dimensional cell aggregates in scaffolds or constructs. The goal of the present study was to generate novel cell aggregate-loaded macroporous scaffolds combining the osteoinductive properties of titanium dioxide (TiO 2 ) with hydroxyapatite-gelatin nanocomposites (HAP-GEL) for regeneration of craniofacial defects. Here we investigated the in vivo applicability of macroporous (TiO 2 )-enriched HAP-GEL scaffolds with undifferentiated and osteogenically differentiated multipotent adult progenitor cell (MAPC and OD-MAPC, respectively) aggregates for calvaria bone regeneration. The silane-coated HAP-GEL with and without TiO 2 additives were polymerized and molded to produce macroporous scaffolds. Aggregates of the rat MAPC were precultured, loaded into each scaffold, and implanted to rat calvaria criticalsize defects to study bone regeneration. Bone autografts were used as positive controls and a poly(lacticco-glycolic acid) (PLGA) scaffold for comparison purposes. Preimplanted scaffolds and calvaria bone from pig were tested for ultimate compressive strength with an Instron 4411 Ò and for porosity with microcomputerized tomography (mCT). Osteointegration and newly formed bone (NFB) were assessed by mCT and nondecalcified histology, and quantified by calcium fluorescence labeling. Results showed that the macroporous TiO 2 -HAP-GEL scaffold had a comparable strength relative to the natural calvaria bone (13.8 -4.5 MPa and 24.5 -8.3 MPa, respectively). Porosity was 1.52 -0.8 mm and 0.64 -0.4 mm for TiO 2 -HAP-GEL and calvaria bone, respectively. At 8 and 12 weeks postimplantation into rat calvaria defects, greater osteointegration and NFB were significantly present in the TiO 2 -enriched HAP-GEL constructs with OD-MAPCs, compared to the undifferentiated MAPCloaded constructs, cell-free HAP-GEL with and without titanium, and PLGA scaffolds. The tissue-engineered TiO 2 -enriched HAP-GEL constructs with OD-MAPC aggregates present a potential useful therapeutic approach for calvaria bone regeneration.

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Section: Discussionmentioning

confidence: 99%

Titanium-Enriched Hydroxyapatite–Gelatin Scaffolds with Osteogenically Differentiated Progenitor Cell Aggregates for Calvaria Bone Regeneration

Ferreira

Padilla

Urkasemsin

et al. 2013

Tissue Engineering Part A

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“…In another study, titanium-coated implants were placed in rat tibia, and the microstructure of peri-implant tissue was examined over time. There was maturation of osseointegration at 28 days after implant placement 28) . In our present study, the results suggest that the experimental implant surface showed more enhanced osseointegration than the titanium implant surface, mainly in the trabecular bone region from early after implant placement until completion of osseointegration.…”

Section: Osteocalcinmentioning

confidence: 99%

“…Thin titanium coating was deposited onto the rods using the method of Watazu reported by Okamatsu et al 27) and Morinaga et al 28) . Briefly, thin titanium coating of approximately 100 nm was deposited by a DC/RF sputtering system (L332S-FHS, Anelva, Tokyo, Japan) with a titanium target (99.9% purity, Kojundo Chemical Lab.…”

Section: Experimental Implantsmentioning

confidence: 99%

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The Effect of Implant Surfaces sputter-coated with Hydroxyapatite Target

Sakai

Okamura

Watazu

et al. 2013

J. Hard Tissue Biology.

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The aim of this study was to develop experimental titanium implants sputter-coated from a hydroxyapatite target and to evaluate peri-implant tissue responses in an animal experiment. The experimental implants were prepared from plastic rods, each of which was 1.6 mm in diameter and 7 mm in length. A thin titanium film was deposited onto the rods by DC magnetron sputtering. The surface of each rod was subsequently sputter-coated from a hydroxyapatite target by RF magnetron sputtering. The experimental implants were placed in the tibiae of 8-week-old male SD rats. Titanium-coated implants were placed as controls using the same method. Tissue samples were obtained 3, 5, 7, 10, 14, and 28 days after implant placement. Histological evaluation was performed using a light microscope, and the bone-implant contact ratios (BICs) were measured. Microstructural observations of implant-bone interfaces were made using a transmission electron microscope (TEM). Peri-implant osteoblastic activity was evaluated using samples obtained 5, 7, and 10 days after implant placement. Immunohistochemical evaluation of type I collagen, osteopontin, and osteocalcin was performed. Uniform smoothness of experimental implant surfaces was confirmed by scanning electron microscope(SEM) images. In the calcium phosphate layer, the compositional ratio of the experimental implant surface was Ca:P:O=1.0:0.79:2.8 based on the results of X-ray photoelectron spectroscopy(XPS). In the trabecular bone region, the BIC ratio was significantly higher in the experimental group than in the control group at 5, 7, 10, and 28 days after implant placement. Type I collagen, osteopontin, and osteocalcin immunostaining revealed that the experimental group tended to show positive results earlier than the control group. The results of this study suggest that surface treatment using a hydroxyapatite target and RF magnetron sputtering can enhance new bone formation during implant osseointegration.

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“…The use of Micro-CT in implant and periimplant bone research has been popular for the past decade, many authors have studied the implant (Schicho et al, 2007), periimplant bone (Rebaudi et al, 2004;Kim et al, 2008;Yoo et al, 2008;Freilich et al, 2009), interface osseointegration and bone-implant integration with the Micro-CT technique (van Oossterwyck et al, 2000;Sennerby et al, 2001;Butz et al, 2006;Morinaga et al, 2008), and have obtained some meaningful results. From Micro-CT scans, one may determine parameters of periimplant bone like bone volume (BV), bone surface (BS), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp), bone connectivity and bone implant integration etc,.…”

Section: Implant and Periimplant Bonementioning

confidence: 99%

State of the Art of Micro‐CT Applications in Dental Research

2009

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This review highlights the recent advances in X-ray microcomputed tomography (Micro-CT) applied in dental research. It summarizes Micro-CT applications in measurement of enamel thickness, root canal morphology, evaluation of root canal preparation, craniofacial skeletal structure, micro finite element modeling, dental tissue engineering, mineral density of dental hard tissues and about dental implants. Details of studies in each of these areas are highlighted along with the advantages of Micro-CT, and finally a summary of the future applications of Micro-CT in dental research is given.

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Chronological Changes in the Ultrastructure of Titanium‐Bone Interfaces: Analysis by Light Microscopy, Transmission Electron Microscopy, and Micro‐Computed Tomography

Abstract: Comparative analysis of the titanium-bone interfaces using light microscopy, TEM, and QCT by micro-CT revealed the precise process of osseointegration.

Cited by 32 publications

References 30 publications

Titanium-Enriched Hydroxyapatite–Gelatin Scaffolds with Osteogenically Differentiated Progenitor Cell Aggregates for Calvaria Bone Regeneration

Titanium-Enriched Hydroxyapatite–Gelatin Scaffolds with Osteogenically Differentiated Progenitor Cell Aggregates for Calvaria Bone Regeneration

The Effect of Implant Surfaces sputter-coated with Hydroxyapatite Target

State of the Art of Micro‐CT Applications in Dental Research

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