Chronological expression of Wnt target genes Ccnd1, Myc, Cdkn1a, Tfrc, Plf1 and Ramp3

Röhrs, Sonja; Kutzner, Nadine; Vlad, Annica; Grünwald, Thomas; Ziegler, Slava; Müller, Oliver

doi:10.1016/j.cellbi.2009.01.016

Cited by 47 publications

(34 citation statements)

References 36 publications

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“…Ramp3 was found to be significantly similar to Tfrc and Tf in GRAIL analysis. Ramp3 and Tfrc are both known to be Wnt target genes (57). Several other genes located in identified putative QTL belong to Wnt pathway: Ankrd6 on Chromosome 4, Hnf1a on Chromosome 5 and Apc on Chromosome 18.…”

Section: Comparison Of Qtl Regions Associated With Liver Iron Levels mentioning

confidence: 99%

In silico QTL mapping of basal liver iron levels in inbred mouse strains

McLachlan

Lee

Steele

et al. 2011

Physiological Genomics

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Both iron deficiency and iron excess are detrimental in many organisms, and previous studies in both mice and humans suggest that genetic variation may influence iron status in mammals. However, these genetic factors are not well defined. To address this issue, we measured basal liver iron levels in 18 inbred strains of mice of both sexes on a defined iron diet and found ∼4-fold variation in liver iron in males (lowest 153 μg/g, highest 661 μg/g) and ∼3-fold variation in females (lowest 222 μg/g, highest 658 μg/g). We carried out a genome-wide association mapping to identify haplotypes underlying differences in liver iron and three other related traits (copper and zinc liver levels, and plasma diferric transferrin levels) in a subset of 14 inbred strains for which genotype information was available. We identified two putative quantitative trait loci (QTL) that contain genes with a known role in iron metabolism: Eif2ak1 and Igf2r. We also identified four putative QTL that reside in previously identified iron-related QTL and 22 novel putative QTL. The most promising putative QTL include a 0.22 Mb region on Chromosome 7 and a 0.32 Mb region on Chromosome 11 that both contain only one candidate gene, Adam12 and Gria1, respectively. Identified putative QTL are good candidates for further refinement and subsequent functional studies.

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Section: Comparison Of Qtl Regions Associated With Liver Iron Levels mentioning

confidence: 99%

In silico QTL mapping of basal liver iron levels in inbred mouse strains

McLachlan

Lee

Steele

et al. 2011

Physiological Genomics

View full text Add to dashboard Cite

show abstract

“…7A), each of which has been implicated in NSC self-renewal (Nakamura et al, 2000;Ohtsuka et al, 2001;Molofsky et al, 2003). By contrast, expression of c-Myc, a common target of the WNT pathway (He et al, 1998;Rohrs et al, 2009), was elevated in NSCs isolated from G-Cat mice (Fig. 7A).…”

Section: Research Articlementioning

confidence: 99%

WNT signaling increases proliferation and impairs differentiation of stem cells in the developing cerebellum

et al. 2012

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SUMMARYThe WNT pathway plays multiple roles in neural development and is crucial for establishment of the embryonic cerebellum. In addition, WNT pathway mutations are associated with medulloblastoma, the most common malignant brain tumor in children. However, the cell types within the cerebellum that are responsive to WNT signaling remain unknown. Here we investigate the effects of canonical WNT signaling on two important classes of progenitors in the developing cerebellum: multipotent neural stem cells (NSCs) and granule neuron precursors (GNPs). We show that WNT pathway activation in vitro promotes proliferation of NSCs but not GNPs. Moreover, mice that express activated -catenin in the cerebellar ventricular zone exhibit increased proliferation of NSCs in that region, whereas expression of the same protein in GNPs impairs proliferation. Although -cateninexpressing NSCs proliferate they do not undergo prolonged expansion or neoplastic growth; rather, WNT signaling markedly interferes with their capacity for self-renewal and differentiation. At a molecular level, mutant NSCs exhibit increased expression of c-Myc, which might account for their transient proliferation, but also express high levels of bone morphogenetic proteins and the cyclin-dependent kinase inhibitor p21, which might contribute to their altered self-renewal and differentiation. These studies suggest that the WNT pathway is a potent regulator of cerebellar stem cell growth and differentiation.

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“…Recently, another TfR-like molecule named TfR2 has been recognized and investigated (11,12), but the exact function of TfR2 remains unclear (8). It has been reported that the expression of TfR1 in mammary epithelial cells exhibits a significant 24-hour rhythm (13). Such rhythmic variation in TfR1 expression seems to affect its iron uptake function resulting in time-dependent changes in the internalization of iron-loaded Tf.…”

Section: Introductionmentioning

confidence: 99%

Circadian Rhythm of Transferrin Receptor 1 Gene Expression Controlled by c-Myc in Colon Cancer–Bearing Mice

et al. 2010

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The abundance of cell surface levels of transferrin receptor 1 (TfR1), which regulates the uptake of ironbound transferring, correlates with the rate of cell proliferation. Because TfR1 expression is higher in cancer cells than in normal cells, it offers a target for cancer therapy. In this study, we found that the expression of TfR1 in mouse colon cancer cells was affected by the circadian organization of the molecular clock. The core circadian oscillator is composed of an autoregulatory transcription-translation feedback loop, in which CLOCK and BMAL1 are positive regulators and the Period (Per), Cryptochrome (Cry), and Dec genes act as negative regulators. TfR1 in colon cancer-bearing mice exhibited a 24-hour rhythm in mRNA and protein levels. Luciferase reporter analysis and chromatin immunoprecipitation experiments suggested that the clock-controlled gene c-MYC rhythmically activated the transcription of the TfR1 gene. Platinum incorporation into tumor DNA and the antitumor efficacy of transferrin-conjugated liposome-delivered oxaliplatin could be enhanced by drug administration at times when TfR1 expression increased. Our findings suggest that the 24-hour rhythm of TfR1 expression may form an important aspect of strategies for TfR1-targeted cancer therapy. Cancer Res; 70(15); 6238-46. ©2010 AACR.

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Chronological expression of Wnt target genes Ccnd1, Myc, Cdkn1a, Tfrc, Plf1 and Ramp3

Cited by 47 publications

References 36 publications

In silico QTL mapping of basal liver iron levels in inbred mouse strains

In silico QTL mapping of basal liver iron levels in inbred mouse strains

WNT signaling increases proliferation and impairs differentiation of stem cells in the developing cerebellum

Circadian Rhythm of Transferrin Receptor 1 Gene Expression Controlled by c-Myc in Colon Cancer–Bearing Mice

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