Lipids extracted from lacustrine deposits in the paleolake Qarhan of the Qaidam basin in the northeastern Tibetan Plateau were determined by conventional gas chromatography-mass spectrometry. Several series of biomarkers were identified, mainly including n-alkanes, n-alkan-2-ones, n-alkanoic acids, branched alkanes, triterpenoids and steroids, indicative of various biogenic contributions. On the basis of cluster analysis, the n-C 15 , n-C 17 , n-C 19 alkanes were proposed to be derived from algae and/or photosynthetic bacteria, the n-C 21 , n-C 23 , n-C 25 homologues from aquatic plants, and the n-C 29 , n-C 31 homologues from vascular plants. In contrast, the n-C 27 alkane is not categorized in the n-C 29 and n-C 31 group of alkanes, probably due to more complex origins including both aquatic and vascular plants, and/or differential biodegradation. Stratigraphically, layers-2, 4 and 5 were found to show a close relationship in n-alkane distribution, associated with a positive shift in carbon isotope composition of bulk organic matter (δ 13 C org ), inferring a cold/dry period. Layers-1 and 6 were clustered together in association with a negative δ 13 C org excursion, probably indicating a relatively warm/humid climate. The potential coupling between the n-alkane distributions and δ 13 C org , suggests a consequence of vegetation change in response to climate change, with the late MIS3 being shown to be unstable, thought to be the climatic optimum in the Tibetan Plateau. Our results suggest that the cluster analysis used in this study probably provides an effective and authentic method to investigate the n-alkane distribution in paleolake sediments.