Chryseobacterium lacus sp. nov. Isolated From the Surface Water of Two Lakes With Light-Induced Carotenoid Production

Abstract

Two Gram-stain-negative, rod-shaped, gliding, catalase-positive, and facultative anaerobic strains, YLOS41 T and XH07, were isolated from surface water of Yilong Lake and West Lake of Dali in Yunnan Province, respectively. Both strains were yellowcolored under light conditions and white-colored under dark conditions. The results of physiological and chemotaxonomic characterization, sequencing and phylogenetic analysis, and draft genome sequence comparison demonstrated that the two strains represented a single novel species within the genus Chryseobacterium, for which the name Chryseobacterium lacus sp. nov. is proposed. The type strain is YLOS41 T (= KCTC 62352 T = MCCC 1H00300 T ), and the second strain is XH07 (= KCTC 62993). During the cultivation process, we found that the colony color of the two strains changed from white to yellow with illumination. The study investigated the effects of light irradiation on the strain YLOS41 T . Results showed that light irradiation did not affect the growth of cells but significantly increased carotenoid synthesis, which caused the change of colony color. In-depth metabolic analysis was conducted by transcriptome. The predominant changes were found for genes involved in carotenoid synthesis as protection from light damage. Based on the genome and transcriptome, we proved that strain YLOS41 T possessed a complete synthetic pathway of carotenoid and speculated that the production was zeaxanthin. This was the first report of Chryseobacterium species with light-induced carotenoid synthesis. This study enhances our present knowledge on how Chryseobacterium species isolated from surface water responds to light damage.

Differentiation of different dibenzothiophene (DBT) desulfurizing bacteria via surface-enhanced Raman spectroscopy (SERS)

“…31 In the present study, all four strains have this biochemical component (carotenoid). 42–45 This peak has a high intensity in Chryseobacterium sp. IS, Mycolicibacterium sp.…”

Jeongeuplla avenae gen. nov., sp. nov., a novel β-carotene-producing bacterium that alleviates salinity stress in Arabidopsis

“…Although enhancing carotenoid production through metabolic engineering has been achieved in microalgae and bacteria, and some carotenoid synthesis enzymes have been cloned ( Sedkova et al, 2005 ; Siddaramappa et al, 2018 ; Zhang J. et al, 2020 ; Chen et al, 2023 ), further research is needed to fully understand the carotenoid biosynthesis pathway and the modifications of the final structure. Accordingly, we next annotated the complete genome of strain DY-R2A-6 T using PGAP, BlastKOALA, and antiSMASH to characterize the carotenoid biosynthesis pathway in this bacterium.…”

Characterization and Performance of Lactate-Feeding Consortia for Reductive Dechlorination of Trichloroethene

“…Meanwhile, homogenizing dispersal also played a certain role ( Figure 2 ) for the operation of transferring to different serum bottles. Subculturing procedure might bring a trace amount of oxygen, which will contribute to the emergence of facultative anaerobic populations such as Hafnia-Obesumbacterium [ 79 ], Psychrobacter [ 80 ], Chryseobacterium [ 81 , 82 ], and Stenotrophomonas [ 83 ]. Simultaneously, supplementation with abundant lactate can increase the number of lactate-metabolizing groups (LMG) represented by the genera of, for example, Pseudomonas , Lactococcus , Brochothrix , Thermus , and Deinococcus ( Figure 5 ).…”