Chryseomicrobium amylolyticum sp. nov., isolated from a semi-arid tropical soil, and emended descriptions of the genus Chryseomicrobium and Chryseomicrobium imtechense

Abstract

A rod-shaped, non-motile, Gram-stain-positive, catalase-positive, starch-hydrolysing strain, JC16T, was isolated from a semi-arid tropical soil from India. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain JC16T clustered with the type species of the genus Chryseomicrobium, Chryseomicrobium imtechense MW 10T, a member of the family Planococcaceae within the phylum Firmicutes with 99.3 % sequence similarity. Major (>10 %) fatty acids of strain JC16T were iso-C15 : 0 and iso-C16 : 0. Minor (<10 and >1 %) amounts of C16 : 0, iso-C14 : 0, anteiso-C15 : 0, iso-C17 : 0, anteiso-C17 : 0, iso-C17 : 1ω10c and C16 : 1ω11c are present in strain JC16T. Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phospholipids (PL2–4), aminolipids (AL1, 2) and an unknown lipid. Cell wall peptidoglycan was of the type l-Orn–d-Glu. The quinone system was composed of MK-7, MK-8 and MK-6. Genomic DNA G+C content of strain JC16T was 57.6 mol%. Distinct physiological, chemotaxonomic and genotypic differences (37 % reassociation based on DNA–DNA hybridization) from Chryseomicrobium imtechense MW 10T support the classification of strain JC16T as a representative of a novel species in the genus Chryseomicobium, for which the name Chryseomicrobium amylolyticum sp. nov. (type strain JC16T = DSM 23442T = NBRC 105215T) is proposed.

Chryseomicrobium palamuruense sp. nov., a haloalkalitolerant bacterium isolated from a sediment sample

“…confirms data already published (Arora et al, 2011;Raj et al, 2013;Deng et al, 2014), and differs from strain PU1 T which contains L-Orn (Fig. S4).…”

“…Phylogenetic examinations strongly suggest that the recently described genus Chryseomicrobium (Arora et al, 2011) comprises the fifteenth genus of this family. Members of the genus Chryseomicrobium are aerobic, Gram-positive, non-endosporeforming rods and, at the time of writing, are represented by only three species with validly published names, Chryseomicrobium imtechense, Chryseomicrobium amylolyticum, and Chryseomicrobium aureum (Arora et al, 2011;Raj et al, 2013 ;Deng et al, 2014). In this paper, we describe a Gramstain-positive, rod-shaped, motile, non-endospore-forming bacterium (strain PU1 T ) that shows 99 % 16S rRNA gene sequence similarity with the type strains of other species of the genus Chryseomicrobium.…”

Characterization and diversity of seed endophytic bacteria of the endemic holoparasitic plant Cistanche armena (Orobanchaceae) from a semi-desert area in Armenia

“…and not sufficiently investigated (Parte, 2018). However, some members of the family Planococcaceae were isolated from a semi-arid tropical soil from India (Raj et al, 2013). Thus, their presence in the examined seeds is not surprising because of the natural habitats of C .…”

Chryseomicrobium aureum sp. nov., a bacterium isolated from activated sludge

“…The genus Chryseomicrobium (Arora et al, 2011), belonging to the family Planococcaceae, was created to accommodate yellow, rod-shaped isolates that included Gram-stainpositive, aerobic, non-motile and non-spore-forming bacteria. At the time of writing, the genus comprises two species with validly published names: Chryseomicrobium imtechense (Arora et al, 2011) and Chryseomicrobium amylolyticum (Raj et al, 2013). In this study, we report on a Chryseomicrobiumlike bacterial strain, designated BUT-2 T , which was isolated from activated sludge.…”

“…At the time of writing, the genus comprises two species with validly published names: Chryseomicrobium imtechense (Arora et al, 2011) and Chryseomicrobium amylolyticum (Raj et al, 2013). In this study, we report on a Chryseomicrobiumlike bacterial strain, designated BUT-2 T , which was isolated from activated sludge.…”