Chrysochromulina sp.: A proposed lipid standard for the algal biofuel industry and its application to diverse taxa for screening lipid content

Bigelow, Nicholas; Barker, Jerry L.; Ryken, Scott A.; Patterson, Johnathan; Hardin, William; Barlow, Steven B.; Deodato, Chloe R.; Cattolico, Rose Ann

doi:10.1016/j.algal.2013.07.001

Cited by 17 publications

(9 citation statements)

References 20 publications

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“…In both strains, the relative proportion of long-chain fatty acids was higher in resting cells compared to vegetative cells, suggesting that H. akashiwo cells appeared to selectively retain some PUFAs over other fatty acid types during resting cell formation. A significant change was particularly noted for eicosapentaenoic acid (C20:5), a fatty acid found in unusually high amounts in H. akashiwo cells [43]. This long chain fatty acid represented approximately 15% of the total fatty acids in vegetative cells, increased to approximately 28% of the total fatty acids in resting cells.…”

Section: Discussionmentioning

confidence: 99%

Behavioral and Physiological Changes during Benthic-Pelagic Transition in the Harmful Alga, Heterosigma akashiwo: Potential for Rapid Bloom Formation

et al. 2013

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Many species of harmful algae transition between a motile, vegetative stage in the water column and a non-motile, resting stage in the sediments. Physiological and behavioral traits expressed during benthic-pelagic transition potentially regulate the timing, location and persistence of blooms. The roles of key physiological and behavioral traits involved in resting cell emergence and bloom formation were examined in two geographically distinct strains of the harmful alga, Heterosigma akashiwo. Physiological measures of cell viability, division and population growth, and cell fatty acid content were made using flow cytometry and gas chromatography – mass spectrometry techniques as cells transitioned between the benthic resting stage and the vegetative pelagic stage. Video-based tracking was used to quantify cell-level swimming behaviors. Data show increased temperature and light triggered rapid emergence from the resting stage and initiated cell swimming. Algal strains varied in important physiological and behavioral traits, including survivorship during life-stage transitions, population growth rates and swimming velocities. Collectively, these traits function as “population growth strategies” that can influence bloom formation. Many resting cells regained the up-swimming capacity necessary to cross an environmentally relevant halocline and the ability to aggregate in near-surface waters within hours after vegetative growth supporting conditions were restored. Using a heuristic model, we illustrate how strain-specific population growth strategies can govern the timescales over which H. akashiwo blooms form. Our findings highlight the need for identification and quantification of strain-specific physiological and behavioral traits to improve mechanistic understanding of bloom formation and successful bloom prediction.

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Section: Discussionmentioning

confidence: 99%

Behavioral and Physiological Changes during Benthic-Pelagic Transition in the Harmful Alga, Heterosigma akashiwo: Potential for Rapid Bloom Formation

et al. 2013

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“…26). The ability to grow this alga reproducibly on a completely defined artificial media, the broad profile of fatty acids produced by the organism, and the ease and reproducibilty of fatty acid extraction suggest this alga might be a good reference standard for fatty acid recovery [87].…”

Section: Lipid-body Formation In Chrysochromulinamentioning

confidence: 99%

Review of the algal biology program within the National Alliance for Advanced Biofuels and Bioproducts

et al. 2017

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In 2010, when the National Alliance for Advanced Biofuels and Bioproducts (NAABB) consortium began, little was known about the molecular basis of algal biomass or oil production. Very few algal genome sequences were available and efforts to identify the best-producing wild species through bioprospecting approaches had largely stalled after the U.S. Department of Energy's Aquatic Species Program. This lack of knowledge included how reduced carbon was partitioned into storage products like triglycerides or starch and the role played by metabolite remodeling in the accumulation of energy-dense storage products. Furthermore, genetic transformation and metabolic engineering approaches to improve algal biomass and oil yields were in their infancy. Genome sequencing and transcriptional profiling were becoming less expensive, however; and the tools to annotate gene expression profiles under various growth and engineered conditions were just starting to be developed for algae. It was in this context that an integrated algal biology program was introduced in the NAABB to address the greatest constraints limiting algal biomass yield. This review describes the NAABB algal biology program, including hypotheses, research objectives, and strategies to move algal biology research into the twenty-first century and to realize the greatest potential of algae biomass systems to produce biofuels.

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“…algal cell morphology, growth conditions such as, temperature, nutrients and photo period) and technological (cultivation, harvesting, extraction and/or conversion) factors [59]. As presented in Fig.…”

Section: Effect Of Algae Strainmentioning

confidence: 99%

Process application of Subcritical Water Extraction (SWE) for algal bio-products and biofuels production

et al. 2015

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Chrysochromulina sp.: A proposed lipid standard for the algal biofuel industry and its application to diverse taxa for screening lipid content

Cited by 17 publications

References 20 publications

Behavioral and Physiological Changes during Benthic-Pelagic Transition in the Harmful Alga, Heterosigma akashiwo: Potential for Rapid Bloom Formation

Behavioral and Physiological Changes during Benthic-Pelagic Transition in the Harmful Alga, Heterosigma akashiwo: Potential for Rapid Bloom Formation

Review of the algal biology program within the National Alliance for Advanced Biofuels and Bioproducts

Process application of Subcritical Water Extraction (SWE) for algal bio-products and biofuels production

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