Chrysophanol-8-O-glucoside, an Anthraquinone Derivative in Rhubarb, Has Antiplatelet and Anticoagulant Activities

Abstract

Abstract. Rhubarb is a widely used traditional medicine and has been reported to elicit a number of biological effects including anti-inflammatory and antiplatelet effects. In the present study, we investigated the effects of anthraquinone derivatives isolated from rhubarb on platelet activity. Of four anthraquinone derivatives isolated from rhubarb examined, chrysophanol-8-O-glucoside (CP-8-O-glc) was found to have the most potent inhibitory effect on collagen-and thrombin-induced platelet aggregation. CP-8-O-glc-treated mice showed significantly prolonged bleeding times. Furthermore, CP-8-O-glc was found to have a significant inhibitory effect on rat platelet aggregation ex vivo and on thromboxane A 2 formation in vitro. In coagulation tests, CP-8-O-glc did not alter prothrombin time, and it prolonged the activated partial thromboplastin time. However, CP-8-O-glc only inhibited platelet phosphatidylserine exposure, but not exert direct inhibition on intrinsic factors. This study demonstrates the antiplatelet and anticoagulant effects of CP-8-O-glc and suggests that this compound might be of therapeutic benefit for the prevention of plateletrelated cardiovascular diseases.

Virtualizing the p-ANAPL Library: A Step towards Drug Discovery from African Medicinal Plants

“…The anthraquinones emodin ( 18 ) and aloe emodin ( 20 ) have demonstrated interesting anticancer properties [54]–[57], while chrysophanol ( 19 ) has shown the ability to induce necrosis through the production of ROS and alteration of ATP levels in J5 human liver cancer cells [58], in addition to its antidiabetic [59], anti-inflammatory [60] and antimicrobial [61]–[62] properties. Moreover, chrysophanol-8- O -glucoside, has shown antiplatelet and anticoagulant activities [63]. It is also proven that physcion ( 17 ) and emodin ( 18 ) exhibit antibacterial properties against three Bacillus species, emodin exhibiting MICs in the range 0.5 – 2.0 μg mL −1 [64].…”

Anthraquinones As Pharmacological Tools and Drugs

“…Anthraquinone derivatives show a wide array of pharmacological activities including laxative, 25,26 anticancer, [27][28][29][30] antiinflammatory, 31 antiarthritic, 32 antifungal, 33 antibacterial, 34 antiviral, 35 antiplatelet, [36][37][38] and neuroprotective effects. 39 In addition, they have shown potential for the treatment of malaria 40 and multiple sclerosis 41 ; and there is continued interest in the development of new anthraquinone derivatives displaying biological activities.…”

An Unusual Cause of Small Bowel Obstruction in a Child: Ingested Rhubarb

“…Although the leaves are toxic, various parts of the plants have medicinal and culinary uses. The traditional Chinese pharmacopeia features rhubarb as a laxative and it has also been reported to elicit a number of biological effects including anti-inflammatory and antiplatelet effects [6]. Rhubarb is grown primarily for its fleshy petioles, commonly known as rhubarb sticks or stalks.…”