Chuanminshen violaceum polysaccharides improve the immune responses of foot-and-mouth disease vaccine in mice

Feng, Haibo; Fan, Jing; Qiu, Hong; Wang, Zhenhua; Yan, Zhiqiang; Liu, Yuan; Guan, Lei; Du, Xiaogang; Song, Zhenhui; Han, Xingfa; Liu, Juan

doi:10.1016/j.ijbiomac.2015.04.044

Cited by 37 publications

(23 citation statements)

References 57 publications

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“…The most significantly enriched GO terms were receptor signaling protein activity including ERBB3, MAP2K5, and MAPK14, GO terms of positive regulation of homeostatic process including FCGR3A, MAPK14, and CEMIP, and receptor signaling protein serine/threonine kinase activity including MAP2K5 and MAPK14 were also significantly activated ( Figure 5C). 9 identify differentially expressed protein (DEP) profiles in serum. Compared with the control group, 39 DEPs were identified in the SO-VE-GS group with 8 up-and 31 downregulated proteins, and in the ISA 206 group, 47 DEPs were identified with 6 up-and 41 downregulated proteins ( Figure 4A-B).…”

Section: Gene Ontology (Go) Enrichment Analysis Of Depsmentioning

confidence: 99%

Enhanced Immune Responses with Serum Proteomic Analysis of Hu Sheep to Foot-and-Mouth Disease Vaccine Emulsified in a Vegetable Oil Adjuvant

et al. 2020

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Our previous study demonstrated that a vegetable oil consisting of soybean oil, vitamin E, and ginseng saponins (SO-VE-GS) had an adjuvant effect on a foot-and-mouth disease (FMD) vaccine in a mouse model. The present study was to compare the adjuvant effects of SO-VE-GS and the conventional ISA 206 on an FMD vaccine in Hu sheep. Animals were intramuscularly (i.m.) immunized twice at a 3-week interval with 1 mL of an FMD vaccine adjuvanted with SO-VE-GS (n = 10) or ISA 206 (n = 9). Animals without immunization served as control (n = 10). Blood was sampled prior to vaccination and at 2, 4, 6, and 8 weeks post the booster immunization to detect FMD virus (FMDV)-specific IgG. Blood collected at 8 weeks after the booster was used for the analyses of IgG1 and IgG2, serum neutralizing (SN) antibody, IL-4 and IFN-γ production, and proteomic profiles. The results showed that IgG titers rose above the protection level (1:128) in SO-VE-GS and ISA 206 groups after 2 and 4 weeks post the booster immunization. At 6 weeks post the booster, the ISA 206 group had 1 animal with IgG titer less than 1:128 while all the animals in the SO-VE-GS group retained IgG titers of more than 1:128. At 8 weeks post the booster, 6 of 9 animals had IgG titers less than 1:128 with a protective rate of 33.3% in the ISA 206 group, while only 1 of 10 animals had IgG titer less than 1:128 with a protective rate of 90% in the SO-VE-GS group, with statistical significance. In addition, IgG1, IgG2, SN antibodies, IL-4, and IFN-γ in the SO-VE-GS group were significantly higher than those of the ISA 206 group. Different adjuvant effects of SO-VE-GS and ISA 206 may be explained by the different proteomic profiles in the two groups. There were 39 and 47 differentially expressed proteins (DEPs) identified in SO-VE-GS compared to the control or ISA 206 groups, respectively. In SO-VE-GS vs. control, 3 immune related gene ontology (GO) terms and 8 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were detected, while 2 immune related GO terms and 5 KEGG pathways were found in ISA 206 vs. control. GO and KEGG analyses indicated that ‘positive regulation of cytokine secretion’, ‘Th1/Th2 cell differentiation’, and ‘Toll-like receptor signaling pathways’, were obviously enriched in the SO-VE-GS group compared to the other groups. Coupled with protein–protein interaction (PPI) analysis, we found that B7TJ15 (MAPK14) was a key DEP for SO-VE-GS to activate the immune responses in Hu sheep. Therefore, SO-VE-GS might be a promising adjuvant for an FMD vaccine in Hu sheep.

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Section: Gene Ontology (Go) Enrichment Analysis Of Depsmentioning

confidence: 99%

Enhanced Immune Responses with Serum Proteomic Analysis of Hu Sheep to Foot-and-Mouth Disease Vaccine Emulsified in a Vegetable Oil Adjuvant

et al. 2020

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“…These components function as antioxidants, antimutations, antifatigue (Chen and Peng 2011), and antivirals , and have expectorant and antitussive properties as well, along with reports of physique enhancement (Feng et al 2015;Zhang et al 2007). The latest study also found that polysaccharides obtained from Chuanminshen could improve the immune responses of foot-and-mouth disease vaccine in mice (Feng et al 2015).…”

Section: Introductionmentioning

confidence: 99%

The complete chloroplast genome sequence and phylogenetic analysis of Chuanminshen (Chuanminshenviolaceum Sheh et Shan)

Yuan

Zhong

Mou

et al. 2016

Physiol Mol Biol Plants

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Chloroplast genome sequences are very useful for species identification and phylogenetics. Chuanminshen (Chuanminshen violaceum Sheh et Shan) is an important traditional Chinese medicinal plant, for which the phylogenetic position is still controversial. In this study, the complete chloroplast genome of Chuanminshen violaceum Sheh et Shan was determined. The total size of Chuanminshen chloroplast genome was 154,529 bp with 37.8% GC content. It has the typical quadripartite structure, a large single copy (17,800 bp) and a small single copy (84,171 bp) and a pair of inverted repeats (26,279 bp). The whole genome harbors 132 genes, which includes 85 protein coding genes, 37 tRNA genes, eight rRNA genes, and two pseudogenes. Thirty-nine SSR loci, 32 tandem repeats and 49 dispersed repeats were found. Phylogenetic analyses results with the help of MEGA showed a new insight for the Chuanminshen phylogenetic relationship with the reported chloroplast genomes in Apiales plants.

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“…T cells and their subpopulations (CD4+ and CD8+ T cells) play a crucial role in immunomodulation, eradicating viruses, preventing viral persistence and inducing the death of tumor cells (Feng et al, 2015;Shen et al, 2013). According to their specific surface molecule, T lymphocytes could differentiate into two different subsets, CD4+ and CD8+ T cell with CD4 and CD8 surface marker, respectively (Shen et al, 2013).…”

Section: Influences Of Hcps On T Lymphocyte Differentiationmentioning

confidence: 99%

“…And CD8+ T cells mediate pathogen clearance, which could recognize and kill the infected host cells (Fan et al, 2016). If the proportion of CD4+ and CD8+ T cells subpopulations increase, the immune responses will also be promoted (Feng et al, 2015). This work, crude HCPS could improve the production of CD4 and CD8 in a dose-dependent manner, indicated that promoting differentiation of T lymphocytes to CD4+ and CD8+ T cells, which could enhance immune response, by enhancing amount of CD4 and CD8 molecules might be another one of the immunomodulatory mechanisms of crude HCPS.…”

Section: Influences Of Hcps On T Lymphocyte Differentiationmentioning

confidence: 99%

“…It has been reported that cluster of differentiation 4+ (CD4+) and CD8+ T cells are key links of immunoregulation of organism (Zhang & Bevan, 2011). Promotion of T lymphocyte differentiation (to CD4+ and CD8+ T cells) by upregulating the expression of CD4+ and CD8+ is also a possible immunoenhancing mechanism of polysaccharides (Feng et al, 2015;Wang, Huang, Sun, & Pan, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation

Qiao

Chuan-bao

Chen

et al. 2017

Food and Agricultural Immunology

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To discuss the molecular mechanism of immunoenhancing activities of Hyriopsis cumingii polysaccharides (HCPS), influences of HCPS on mice immunosignaling molecules (IL-2, IL-10, IFN-γ, NO, cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP)) and T lymphocyte differentiation (to CD4 + and CD8+ T cells) were evaluated by cell model in vitro and/or cyclophosphamide-induced immunosuppression animal model in vivo. Results showed that gastric gavage of crude HCPS could promote the production of IL-2, IFN-γ, inducible nitric oxide synthase (iNOS), cGMP, CD4 and CD8 in a dose-dependent manner. While, crude HCPS by gastric gavage exhibited suppressive effects on the production of IL-10 and cAMP in a dose-dependent manner. Crude HCPS and its purified fractions (HCPS-1, HCPS-2 and HCPS-3) could strengthen peritoneal macrophage expressing iNOS in vitro in a dose-dependent manner. HCPS-stimulated immunostrengthening functions was mediated, at least in part, by immunosignaling molecules of IL-2, IL-10, IFN-γ, NO, cAMP and cGMP, and T lymphocyte differentiation (to CD4+ and CD8+ T cells). ARTICLE HISTORY

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Chuanminshen violaceum polysaccharides improve the immune responses of foot-and-mouth disease vaccine in mice

Cited by 37 publications

References 57 publications

Enhanced Immune Responses with Serum Proteomic Analysis of Hu Sheep to Foot-and-Mouth Disease Vaccine Emulsified in a Vegetable Oil Adjuvant

Enhanced Immune Responses with Serum Proteomic Analysis of Hu Sheep to Foot-and-Mouth Disease Vaccine Emulsified in a Vegetable Oil Adjuvant

The complete chloroplast genome sequence and phylogenetic analysis of Chuanminshen (Chuanminshenviolaceum Sheh et Shan)

Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation

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