Chungsimyeonja-eum inhibits inflammatory responses in RAW 264.7 macrophages and HaCaT keratinocytes

Lim, Hye‐Sun; Kim, Yeji; Seo, Chang‐Seob; Yoo, Sae‐Rom; Jin, Seong-Eun; Shin, Hyeun‐Kyoo; Jeong, Soo‐Jin

doi:10.1186/s12906-015-0902-2

Cited by 13 publications

(10 citation statements)

References 29 publications

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“…HaCaT cells. The transcription factor STAT1 serves an important role in the regulation of skin inflammation and is associated with chemokine production in keratinocytes that have been treated with inflammatory stimuli (18,23). In the present study, TNF-α and IFN-γ stimulation notably increased the expression levels of p-STAT1 compared with the untreated HaCaT control cells (Fig.…”

Section: Effects Of Hss On the Activation Of Stat1 In Tnf-α-and Ifn-γsupporting

confidence: 53%

“…Thus, it is necessary to develop anti-inflammatory drugs with improved efficacy against skin inflammation, with fewer side effects, that may be used over longer periods of time. Recently, various studies have reported on the inhibitory effects of traditional herbal formulas on skin inflammation (14)(15)(16)(17)(18) and have indicated that herbal formulas may be valuable alternative medicines for treating skin inflammation diseases. Hyangso-san (HSS) is a traditional herbal formula that has been used in Korea to treat symptoms of the common cold, including headache, pantalgia, fever and chills (19).…”

Section: Potential Inhibitory Effects Of the Traditional Herbal Prescmentioning

confidence: 99%

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Potential inhibitory effects of the traditional herbal prescription Hyangso-san against skin inflammation via inhibition of chemokine production and inactivation of STAT1 in HaCaT keratinocytes

Lim

Lee

et al. 2017

Mol Med Report

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Inflammatory skin disease are caused by multiple factors, including susceptibility genes, and immunologic and environmental factors, and are characterized by an increase in epidermal thickness and the infiltration of macrophages, keratinocytes, mast cells, eosinophils and other inflammatory cells. Keratinocytes may serve an important role in the pathogenesis of inflammatory skin diseases. The traditional herbal decoction Hyangso‑san (HSS) has been used to treat symptoms of the common cold, including headache, pantalgia, fever and chills. However, to the best of our knowledge, there is no evidence regarding whether HSS has an effect on inflammatory skin diseases. The present study investigated the anti‑skin inflammation activity of HSS using the HaCaT human keratinocyte cell line. The mRNA expression and production of inflammatory chemokines, including C‑C motif chemokine ligand 22 (CCL22), CCL5, CCL17, and interleukin (IL)‑8, was measured using reverse transcription polymerase chain reaction and ELISA analyses. Moreover, we evaluated the effect of HSS on signal transducer and activator of transcription 1 (STAT1) pathway in HaCaT cells. The cells were stimulated with tumor necrosis factor‑α (TNF‑α) and interferon‑γ (IFN‑γ) to induce an inflammatory reaction. In the TNF‑α‑ and IFN‑γ‑stimulated cells, the production and expression of inflammatory chemokines were observed, including CCL22, CCL5, CCL17 and IL‑8. In addition, stimulation with TNF‑α and IFN‑γ increased the phosphorylation and nuclear translocation of STAT1 in HaCaT cells. By contrast, HSS extract treatment inhibited TNF‑α‑ and IFN‑γ‑induced STAT1 activation. Results from the present study indicated that HSS exhibited inhibitory effects on TNF‑α‑ and IFN‑γ‑mediated chemokine production and expression by targeting STAT1 in keratinocytes. Overall, the results indicated that HSS may be a potential candidate therapeutic drug for inflammatory skin diseases such as atopic dermatitis.

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Section: Effects Of Hss On the Activation Of Stat1 In Tnf-α-and Ifn-γsupporting

confidence: 53%

Section: Potential Inhibitory Effects Of the Traditional Herbal Prescmentioning

confidence: 99%

Potential inhibitory effects of the traditional herbal prescription Hyangso-san against skin inflammation via inhibition of chemokine production and inactivation of STAT1 in HaCaT keratinocytes

Lim

Lee

et al. 2017

Mol Med Report

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“…The membrane was washed three times with TBST at intervals of 10 min and developed using the SuperSignal West Femto Maximum Sensitivity Substrate (ultrasensitive enhanced chemiluminescent substrate) (NCI34095KR; Thermo Fisher Scientific Inc., Waltham, MA, USA). Subsequently, membranes were photographed and, densitometric band values were determined using the commercially available ChemiDoc XRS + Imaging System for quantitative analyses (Bio-Rad Laboratories) as described previously [16].…”

Section: Immunoblottingmentioning

confidence: 99%

Inhibitory effects of Ponciri Fructus on testosterone-induced benign prostatic hyperplasia in rats

Jeon

Ohn

Seo

et al. 2017

65th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA 2017)

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Background: Benign prostatic hyperplasia (BPH) is non-cancerous condition of enlargement of the prostate, a common occurrence in older men. The immature fruits of Poncirus trifoliata (L.) Rafinesque (Rutaceae), Ponciri Fructus are widely used in traditional oriental medicine for the therapy of various diseases. However, little is known about the mechanism underlying the pathogenesis of BPH. In the present study, we investigated the protective effects of a Ponciri Fructus extract (PFE) on the development of BPH in a in a rat model of BPH induced by testosterone propionate (TP). Methods: Male Sprague Dawley rats were used as a model of BPH after its induction by daily subcutaneous injections of TP/corn oil, for a period of four weeks. PFE was administrated daily 1 h before TP/corn oil injection by oral gavage at a dose level of 200 mg/kg during the 4 weeks of TP/corn oil injections. All rats were sacrificed at the end of the experiment, we measured the relative prostate weight, the levels of testosterone and dihydrotestosterone (DHT), histological changes, activities of antioxidant enzymes (catalase, glutathione peroxidase, glutathione reductase, and superoxide dismutase), and expression of proliferating cell nuclear antigen (PCNA). In addition, we also measured the inhibition (%) of 5α-reductase in the prostatic tissue.

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“…In addition, tumor necrosis factor (TNF)-αincreases serum IgE levels, resulting in increased levels of inflammatory chemokines and cytokines. These inflammatory effects can be reversed by treatment with antiinflammatory candidates that inhibit signal transducer and activators of transcription (STAT) and nuclear factor (NF)-κB signaling pathways [11][12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Anti-Inflammatory Effects of Bee Venom on Phthalic Anhydride-Induced Atopic Dermatitis

Oh¹

2020

J Acupunct Res

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Background: Atopic dermatitis (AD) is a chronic inflammatory condition which can be studied using phthalic anhydride (PA) to induce AD. Anti-inflammatory properties of bee venom (BV) wereinvestigated to determine whether it may be a useful treatment for AD. Methods: AD was induced by applying to pical PA to 8-week-old HR-1 mice (N = 50), then treating with (0.1, 0.25, and 0.5 µg) or without topical BV. Body weight, ear thickness histology, enzymelinked immune sorbent assay (serum IgE concentrations), Western blot analysis [inducible nitric oxide synthase, cyclooxygenase-2, IκB-α, phospho-IκB-α, c-Jun N-terminal kinase (JNK), phospho-JNK, p38, phospho-p38, extra cellular signal-regulated kinase (ERK), and phospho-ERK], and the pull down assay for immunoblotting (p50), were used to measure inflammatory mediators. Results: PA + BV (0.1, 0.25, and 0.5 μg) significantly decreased ear thickness without altering body weight. IgE concentrations decreased in the PA + BV (0.5 µg)-treated groups compared with PAtreatment. Tumor necrosis factor-α, interleukin-1β, inducible nitric oxide synthase, cyclooxygenase-2, phospho-IκB-α, phospho-JNK, p38, phospho-p38, and phospho-ERK, all decreased following treatment with PA + BV compared with the PA-treatment alone. p50 was upregulated in the PA + BV-treated groups compared with the PA-treated group. Furthermore, the number of mast cells decreased in the PA + BV-treated groups compared with the PA-treated group. Epidermal thickness was significantly lower in the PA + BV-treated group compared with PA treatment alone. Conclusion: BV maybe a useful anti-inflammatory treatment for AD.

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Chungsimyeonja-eum inhibits inflammatory responses in RAW 264.7 macrophages and HaCaT keratinocytes

Cited by 13 publications

References 29 publications

Potential inhibitory effects of the traditional herbal prescription Hyangso-san against skin inflammation via inhibition of chemokine production and inactivation of STAT1 in HaCaT keratinocytes

Potential inhibitory effects of the traditional herbal prescription Hyangso-san against skin inflammation via inhibition of chemokine production and inactivation of STAT1 in HaCaT keratinocytes

Inhibitory effects of Ponciri Fructus on testosterone-induced benign prostatic hyperplasia in rats

Anti-Inflammatory Effects of Bee Venom on Phthalic Anhydride-Induced Atopic Dermatitis

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