In recent decades, several eco-lodges have been established in biodiversity areas of Ethiopia, but quantitative scientific information that helps guide the sustainable management of eco-lodges is lacking. This study was the first attempt that examined the role of the GCEL for the conservation of wild mammals, birds, and woody plants. The objective of the study was to characterize and quantify species diversities and densities of wild mammals, birds, and woody plants in the compound of the eco-lodge and in the adjacent field. Following a total of six randomly aligned transects, field data on the species richness and abundance of wild mammals and birds across the landscape were collected in the compound of the GCEL (here after the EHH) versus in the adjacent field (here after the FGH).Moreover, a systematic sampling design was employed to lay a total of 39 circular plots (i.e, each with a radius of 10m) and collect data on the species richness and abundance of woody plants. The results revealed that species richness, diversity, and evenness of wild mammals were 11, 1.6 and 0.67, respectively, in the EHH and 11, 1.4 and 0.6, respectively, in the FGH. Similarly, species richness, diversity and evenness of birds were 33, 3.3 and 0.96, respectively, in the EHH, and 29, 3 and 0.88, respectively, in the FGH. The woody species were represented by 16, 2.7 and 0.97 species richness, diversity, and evenness, respectively, in the EHH, and 16, 2.6 and 0.94 species richness, diversity, and evenness, respectively, in the FGH. The total mean densities of wild mammals recorded in the EHH and FGH were 16.0 and 7.5 individuals, respectively. Similarly, the total mean densities of birds computed in the EHH and FGH were 668.5 and 158.3 individuals, respectively. Moreover, the total mean densities of woody species estimated in the EHH and FGH were 37,665.2 and 16,845.6 individuals, respectively. The findings of this study generated quantitative scientific information for policy-makers and planners that guides them toward better and more informed decision-making for eco-lodge establishment and management that is geared toward biodiversity conservation.