Cianobactérias de ambiente costeiro: filogenia, prospecção gênica e química de moléculas bioativas

Vaz, Marcelo Gomes Marçal Vieira

doi:10.11606/t.11.2014.tde-17092014-163006

Cited by 2 publications

(4 citation statements)

References 75 publications

(129 reference statements)

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“…Segundo os autores, esse gênero de cianobactérias é dominante nos ecossistemas marinhos e a produção dessas vesículas suporta o crescimento de bactérias heterotróficas influenciando o fluxo de carbono nesse ambiente. Apesar da grande variedade morfológica, filogeneticamente as cianobactérias representam um grupo coerente com o domínio Bacteria (BONEN, 1979;SHI;FALKOWSKI, 2008;VAZ, 2014).…”

Section: Cianobactériasunclassified

Potencial de cianobactérias cultivadas em meio suplementado com vinhaça para produção de biodiesel

Souza¹

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Cyanobacteria are photoautotrophic prokaryotes that show a wide morphological and metabolic variety, which favors the colonization of the most diverse types of environments. This versatility reflects in its numerous biotechnological applications in areas such as agriculture, energy, nutrition and effluent treatment. With the increasing in energy and food demand, cyanobacteria are considered a more sustainable alternative for the biosynthesis of products of industrial interest. In this context, the aim of this study was to evaluate the cyanobacteria growth in media supplemented with vinasse and its effect on lipid production and fatty acid composition, with focus on biodiesel production. So, eleven cyanobacterial strains from water samples from Amazonian rivers were morphologically and genetically identified as belonging to four orders-Nostocales, Chroococciodipsidales, Synecoccocales e Oscillatoriales. These strains had their growth evaluated in culture media supplemented with vinasse (1, 2,5, 5 and 10% v/v) and in vinasse diluted in ultrapure water (2,5, 5 and 10% v/v). All strains were able to grow at the concentrations tested, however, at higher vinasse concentrations, growth inhibition and/or higher heterotrophic contaminants proliferation was observed. In vinasse diluted in ultrapure water, none of them were able to grow. Two cyanobacterial strains were selected for subsequent analysis due to rapid growth (Planktothrix mougeotii CMAA1564) and favorable fatty acid profile for biodiesel production (Amazoninema brasiliense CMAA1602, a new genus). Then, these strains had their gowth investigated in the media BG11, BG11+1% and BG11+2,5% of vinasse using the gravimetric (dry biomass) and spectrophotometric (optical density-OD680nm e OD750nm) methods, for growth phases identification. The highest lipid and biomass productivities were observed in the biomass collected in the log phase in medium supplemented with 1% of vinasse, wtith the highest values observed for the P. mougeotii CMAA1564 (3,18 e 35,87 mg L-1 dia-1 , respectively). The fatty acid profile of the lipid fraction showed the predominance of saturated and monounsaturated fatty acids in the oils of biomass grown in vinasse medium, with palmitic, palmitoleic, oleic and alpha-linolenic acids being the majority. From the fatty acid composition of the oils, it was possible to estimate eleven quality parameters of the biodiesel derived from these lipid feedstocks. Most of the properties evaluated werein accordance with the biodiesel quality specification estabilished in the national and international legislation, except for the kinematic viscosity that presented lower values. These results are promising given the opportunity for exploiting the underestimated cyanobacteria diversity for biotechnological purposes. This research shows the feasibility of using the lipid content of these organisms as an alternative lipid feedstock for biodiesel production.

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Section: Cianobactériasunclassified

Potencial de cianobactérias cultivadas em meio suplementado com vinhaça para produção de biodiesel

Souza¹

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“…As toxinas de cianobactérias constituem uma importante fonte de produtos naturais encontradas em ambientes aquáticos (CARMICHAEL, 1997). Alguns estudos encontraram genes em Oxynema, que participam na síntese de cianotoxinas tipo saxitoxinas e microcistinas (VAZ, 2014;SILVA et al, 2014). Visando essa possibilidade, realizou-se a busca de genes envolvidos à síntese de cianotoxinas nas sequências montadas do genoma, comparando a homologia dos genes candidatos por análise de domínios conservados.…”

Section: Cianotoxinasunclassified

“…Essencialmente, oito proteínas codificadas por esses genes (sxtA, sxtG, sxtB, sxtD, sxtS, sxtU, sxtH/T e sxtI) parecem estar envolvidas diretamente na síntese de STX (KELLMANN et al, 2008;PEARSON et al, 2010;HACKETT et al, 2013). Entre eles, os genes sxtI e sxtA foram detectados por sequenciamento de produtos de PCR, sugerindo a síntese da saxitoxina pela linhagem (VAZ, 2014;SILVA et al, 2014). No entanto, somente os genes sxtH/sxtT, sxtW e sxtO, que codificam para fenilpropionato de dioxigenase, ferredoxina 4-Fe e 4-S e adenililsulfato quinase (KELLMANN et al, 2008) (SHI et al, 2009).…”

Section: Cianotoxinasunclassified

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Taxonomia, genômica e potencial funcional da cianobactéria >i<Oxynema>/i< sp. CENA135

Castillo¹,

Angélica²

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e em especial a Fernanda Rios Jacinavicius pela guia, ensinamentos e disponibilidade na realização de análises. Às funcionarias do laboratório Ana Paula Dini Andreote e Renata Beatriz Cruz, pelas distintas atividades que realizam no laboratório e ainda assim me forneceram apoio. Aos colegas do laboratório, amigos, com todo carinho e gratidão para Bruno Evangelista de Souza, Taiane Fernanda Barradas, Endrews Delbaje, Caroline Rosa de Carvalho e Rafael Barty Dextro. Em especial para Juliana dos Santos Costa e Thierry Alexandre Pellegrinetti pelo companherismo e o suporte em momentos cinzentos. A nossa Pós-doutoranda Simone Cotta e às estagiárias Julia Polezi Formagio e Lara Losovoi pela juda recebida. Ao pesquisador Danillo Alvarenga pelos conselhos fornecidos A Guilherme K. Hosaka, pelo amor, amizade, companhia, ensinamentos, apoio inestimável durante minha formação, e pelo Pisco. A sua familia pelas atenções recebidas e as sempre interessantes conversas culturais. A Rodolfo Silva e Fernando Matias, pela amizade, apoio e aventuras neste lindo país. Ao Programa de Pós-Graduação em Microbiologia Agrícola e ao Serviço da Pós-Graduação, em especial a Maria Solizete Granziol Silva e Alexandre Joviniano dos Santos, por todo o auxílio e orientação fornecida. O presente trabalho foi realizado com apoio da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES)-Código de Financiamento 001 ABSTRACT Taxonomy, genomics and functional potential of the cyanobacteria Oxynema sp. CENA135 Oxynema is a derivated genus from the polyphyletic Phormidium and with few registered species, which were found in mangroves, marine or high salinity environments. The taxonomic and genomic informations are almost nonexisting for this genus, with a few exceptions of 16S rRNA and 16S-23S ITS sequences available at databases. Previous studies performed with Oxynema sp. CENA135 isolated from a Brazilian mangrove showed its potential to synthesize siderophores, aliphatic hydrocarbons, anticancer and antibiotic substances, besides its effective degradation of some types of textile dyes. In this work, the strain Oxynema sp. CENA135 was analyzed under a polyphasic approach (morphology, habitat, phylogenomics, phylogeny of the 16S rRNA gene and secondary structure of 16S-23S ITS), including the search for genes or gene clusters related to the biosynthesis of natural products and the detection of associated bacteria. After morphological characterization using an optic and a scanning microscopes, the genomic sequencing was performed from a mate pair library at the HiSeq (Illumina) platform. This is the first genome of the genus Oxynema and, therefore, for its assembly it was used a de novo method resulting in 11 scaffolds in a total size of 6.2 Mbp, with N50 of 3,591,108 pb, GC content of 51.6% and average coverage of 190x. The checkM software estimated a high completeness of the genome (99.29%) and few contaminations (1.22%). Genome mining revealed a biorremediation potential due to the presence of genes related to resistance of copper,...

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Cianobactérias de ambiente costeiro: filogenia, prospecção gênica e química de moléculas bioativas

Cited by 2 publications

References 75 publications

Potencial de cianobactérias cultivadas em meio suplementado com vinhaça para produção de biodiesel

Potencial de cianobactérias cultivadas em meio suplementado com vinhaça para produção de biodiesel

Taxonomia, genômica e potencial funcional da cianobactéria >i<Oxynema>/i< sp. CENA135

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