Ciclo de vida de Triatoma dimidiata Latreille, 1811 (Hemiptera, Reduviidae) en condiciones de laboratorio: producción de ninfas para ensayos biológicos

Abstract

Introducción. A pesar de la importancia de Triatoma dimidiata como vector de la enfermedad de Chagas, poco se conoce de su ciclo biológico y de la producción eficiente de insectos disponibles para ensayos biológicos. Objetivo. Determinar las características del ciclo de vida en el laboratorio y establecer las condiciones del estado nutricional para la producción eficiente de ninfas de V estadio para ensayos biológicos. Materiales y métodos. Se determinaron los tiempos de desarrollo de los estadios de ninfa en condiciones controladas de laboratorio hasta alcanzar la fase adulta. Se llevó a cabo una cría masiva de ninfas de V estadio, alimentadas y pesadas después de diferentes periodos de ayuno, distribuidas en rangos de peso para obtener la mayor proporción de individuos. Resultados. El tiempo medio de paso de huevo a adulto fue de 269 días, con un amplio rango de duración (174 a 598 días) y, para los estadios I, II, II, IV y V, fue de 33, 37, 41, 61 y 69 días, respectivamente, con una mortalidad de 22%. Se obtuvo una eficiencia de 76% en ninfas de V estadio, alimentadas después de 22 días de ayuno, en el rango de 201 a 300 mg de peso. Conclusión. T. dimidiata presentó un tiempo de desarrollo intermedio entre los triatominos con amplio rango para algunos individuos, posiblemente debido a la irregularidad en su alimentación. La identificación de un rango de peso homogéneo después de 22 días de ayuno con gran producción de ninfas de V estadio, facilita la aplicación de protocolos estandarizados para establecer criterios de selección de compuestos insecticidas utilizables en los programas de control.Palabras clave: Triatominae, triatoma, etapas del ciclo de vida, enfermedad de Chagas, Colombia.Life cycle of Triatoma dimidiata Latreille, 1811 (Hemiptera, Reduviidae) under laboratory conditions: production of nymphs for biological tests Introduction. Despite the importance of Triatoma dimidiata as a vector of Chagas disease, little is known of its life cycle and the efficient production of these insects for biological tests. Objective. Life cycle characteristics in the laboratory were described and optimum nutritional conditions were established for the efficient production of nymphs V stage for biological tests. Materials and methods. We determined the time of development of the nymphal stage under controlled laboratory conditions until reaching the adult stage. In a massive rearing of stage V nymphs, fed and weighted after varying periods of fasting, distributed in weight ranges to obtain the largest proportion of individuals. Results. The time mean from egg to adult was 269 days, with a wide range of duration (174 to 598 days) and the times required for 1st, 2nd, 3rd, 4th and 5th stage development was 33, 37, 41, 61 and 69 days, respectively, with a mortality of 22%. The optimum treatment was 22 days of fasting, in which 76% of the nymphs reached stage V with a weight range from 201 to 300 mg. Conclusion. Triatoma dimidiata presented development time with broad range for some individuals, possibly due to the irreg...

Bionomics of populations ofMeccus pallidipennis(Stål), 1872 (Hemiptera: Reduviidae) from Mexico

“…Those differences in the potential number of vector-human contacts might help explain the greater incidence of human serological cases in the states of Michoacán and Oaxaca (>8,000 per year) compared with Morelos (<2,000 per year) (Ramsey et al 2003). Mortality rates were low and similar to those of some related triatomine species, such as T. maculata, Erichson, 1848, T. dimidiata Latreille, 1811, and M. longipennis (Feliciangeli and Rabinovich 1985, Reyes and Angulo 2009, Martínez-Ibarra et al 2012a) and to M. pallidipennis in a previous study (Martínez-Ibarra and Novelo-López 2004). Most of these species are considered to be important vectors of Chagas disease in their distribution areas (Salazar-Schettino et al 2010).…”

Life cycle and vectorial competence of Triatoma williami (Galvão, Souza e Lima, 1965) under the influence of different blood meal sources

“…However, it was observed that the duration of N3 was shorter in both groups. This reduction in the third stage was not observed in the cycles of T. arthurneivai (Juarez, 1970), T. dimidiata (Reyes & Angulo, 2009), T.carcavalloi (Cardozo-de-almeida et al, 2014), but it occurred with T.…”

The biology of three Mexican-American species of Triatominae (Hemiptera: Reduviidae): Triatoma recurva, Triatoma protracta and Triatoma rubida

“…As for various previously studied triatomine species, such as Triatoma brasiliensis Neiva, Triatoma dimidiata (Latreille) and Triatoma ryckmani Zeledón and Ponce (Daflon-Texeira et al 2009, Reyes & Angulo 2009, Zeledón et al 2010, Reyes-Novelo et al 2011, the egg eclosion rate was greater than 70% for two of the three species studied, the exception being T. recurva. All of the species studied had average incubation periods of approximately 19 days, reflecting the favourability of the maintenance conditions for the development of these species.…”