CIDEA Regulates De Novo Fatty Acid Synthesis in Bovine Mammary Epithelial Cells by Targeting the AMPK/PPARγ Axis and Regulating SREBP1

Cheng, Ji; Xu, Dianwen; Chen, Lisha; Guo, Wenjin; Hu, Guiqiu; Liu, Juxiong; Fu, Shoupeng

doi:10.1021/acs.jafc.2c05226

Cited by 11 publications

(13 citation statements)

References 49 publications

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“…Sodium acetate promotes the SREBP1 pathway to increase milk fat synthesis in dairy cows by inhibiting TATA component regulatory factor 1 (TMF1) . Overexpression of cell-death-induced DNA fragmentation factor α-like effector A (CIDEA) promoted nuclear translocation of SREBP1, increasing milk fat accumulation in mammary epithelial cells . SA is a key substrate for de novo synthesis, in cultured mammary epithelial cells of dairy cows, the extracellular supply of SA can significantly increase intracellular milk fat synthesis .…”

Section: Discussionmentioning

confidence: 99%

“…25 Overexpression of cell-death-induced DNA fragmentation factor α-like effector A (CIDEA) promoted nuclear translocation of SREBP1, increasing milk fat accumulation in mammary epithelial cells. 26 SA is a key substrate for de novo synthesis, in cultured mammary epithelial cells of dairy cows, the extracellular supply of SA can significantly increase intracellular milk fat synthesis. 23 In our study, SA promoted an increase in FASN and SREBP1 levels in BMECs, leading to the accumulation of TAG and an increase in intracellular lipid droplets.…”

Section: ■ Discussionmentioning

confidence: 99%

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MSI2 Modulates Unsaturated Fatty Acid Metabolism by Binding FASN in Bovine Mammary Epithelial Cells

Lyu,

Meng,

Che

et al. 2023

J. Agric. Food Chem.

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Section: Discussionmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%

MSI2 Modulates Unsaturated Fatty Acid Metabolism by Binding FASN in Bovine Mammary Epithelial Cells

Lyu,

Meng,

Che

et al. 2023

J. Agric. Food Chem.

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“…Adipose metabolism refers to the processes involved in the uptake, storage, and release of lipids in adipose tissue. Consistently, a study has shown that cell-death-inducing DNA fragmentation factor-α-like effector A (CIDEA) by inhibiting the activity of AMP-activated protein kinase (AMPK) which is followed by enhancement of SREBP1 and PPARs which are important regulators of lipid metabolism in the mammary gland of cows, and are involved in the synthesis of milk fat ( Cheng et al, 2022 ). Furthermore, they investigated the role of CIDEA in regulating de novo fatty acid synthesis in bovine mammary epithelial cells (BMECs).…”

Section: Genetic Regulation Of Mtor Signaling Pathway For Milk Fat An...mentioning

confidence: 93%

“…Furthermore, they investigated the role of CIDEA in regulating de novo fatty acid synthesis in bovine mammary epithelial cells (BMECs). The authors found that CIDEA promotes de novo fatty acid synthesis in BMECs by regulating the AMPK/PPARγ axis and SREBP1 ( Cheng et al, 2022 ). Specifically, CIDEA enhances the synthesis of fatty acids, which are used to produce milk fat.…”

Section: Genetic Regulation Of Mtor Signaling Pathway For Milk Fat An...mentioning

confidence: 99%

Genetics, environmental stress, and amino acid supplementation affect lactational performance via mTOR signaling pathway in bovine mammary epithelial cells

Li,

Khan,

Khan

et al. 2023

Front. Genet.

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Mammary glands are known for their ability to convert nutrients present in the blood into milk contents. In cows, milk synthesis and the proliferation of cow mammary epithelial cells (CMECs) are regulated by various factors, including nutrients such as amino acids and glucose, hormones, and environmental stress. Amino acids, in particular, play a crucial role in regulating cell proliferation and casein synthesis in mammalian epithelial cells, apart from being building blocks for protein synthesis. Studies have shown that environmental factors, particularly heat stress, can negatively impact milk production performance in dairy cattle. The mammalian target of rapamycin complex 1 (mTORC1) pathway is considered the primary signaling pathway involved in regulating cell proliferation and milk protein and fat synthesis in cow mammary epithelial cells in response to amino acids and heat stress. Given the significant role played by the mTORC signaling pathway in milk synthesis and cell proliferation, this article briefly discusses the main regulatory genes, the impact of amino acids and heat stress on milk production performance, and the regulation of mTORC signaling pathway in cow mammary epithelial cells.

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“…High levels of NEFA seem to stimulate the expression of the CIDEA gene, and consequently increase fatty acid synthesis de novo and milk fat secretion [87]. CIDEA was recently described as a regulator of de novo fatty acid synthesis in cattle as well [88]. In general, high levels of NEFA are associated with ketosis and fatty liver, poor reproductive performance, and negative energy balance in early lactation (reviewed by [89]).…”

Section: Candidate Genes For Fat Yieldmentioning

confidence: 99%

Sequence-based GWAS in 180 000 German Holstein cattle reveals new candidate genes for milk production traits

Križanac,

Reimer,

Heise

et al. 2023

Preprint

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BackgroundThe use of genome-wide association studies (GWAS) has led to the identification of numerous quantitative trait loci and candidate genes in dairy cattle. To obtain sufficient power of GWAS and to identify quantitative trait nucleotides, whole-genome sequence data is required. Sequence data facilitates the identification of potential causal variants; however, sequencing of whole genomes is still expensive for a large number of animals. Imputation is a quick and efficient way of obtaining sequence data from large datasets. Milk production traits are complex and influenced by many genetic and environmental factors. Although extensive research has been performed for these traits, with many associations unveiled thus far, due to their crucial economic importance, complex genetic architecture, and the fact that causative variants in cattle are still scarce, there is a need for a better understanding of their genetic background. In this study, we aimed to identify new candidate loci associated with milk production traits in German Holstein cattle, the most important dairy breed in Germany and worldwide. For that purpose, 252,285 cattle were imputed to the sequence level and large-scale GWAS was carried out to identify new association signals.ResultsWe confirmed many known and identified 30 previously unreported candidate genes for milk, fat, and protein yield. While all of the genes were functionally associated with the traits, some showed pleiotropic effects as well. Specifically, association with mammary gland development, fatty acid synthesis, metabolism of lipids, or milk production QTLs in other farm animals has been reported. Variants associated with these genes explained a large percentage of genetic variance, compared to random ones.ConclusionsOur findings proved the power of large samples and sequence-based GWAS in detecting new association signals. In order to fully exploit the power of GWAS, one should aim at very large samples combined with whole-genome sequence data. Although milk production traits in cattle are comprehensively researched, the genetic background of these traits is still not fully understood, with the potential for many new associations to be revealed, as shown in our study. With constantly growing sample sizes, we expect more insights into the genetic architecture of production traits in the future.

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CIDEA Regulates De Novo Fatty Acid Synthesis in Bovine Mammary Epithelial Cells by Targeting the AMPK/PPARγ Axis and Regulating SREBP1

Cited by 11 publications

References 49 publications

MSI2 Modulates Unsaturated Fatty Acid Metabolism by Binding FASN in Bovine Mammary Epithelial Cells

MSI2 Modulates Unsaturated Fatty Acid Metabolism by Binding FASN in Bovine Mammary Epithelial Cells

Genetics, environmental stress, and amino acid supplementation affect lactational performance via mTOR signaling pathway in bovine mammary epithelial cells

Sequence-based GWAS in 180 000 German Holstein cattle reveals new candidate genes for milk production traits

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