CIF150, a Human Cofactor for Transcription Factor IID-Dependent Initiator Function

Kaufmann, Jörg; Ahrens, Klaus; Koop, Ronald; Smale, Stephen T.; Müller, Rolf

doi:10.1128/mcb.18.1.233

Cited by 59 publications

(75 citation statements)

References 38 publications

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“…First, there is considerable evidence that TFIID binds to the Inr in a sequence-specific manner (e.g., see Kaufmann and Smale 1994;Martinez et al 1994;Purnell et al 1994;Burke and Kadonaga 1996;Oelgeschläger et al 1996). More specifically, it appears that TAF II 150 and TAF II 250 (i.e., TAF2 and TAF1; Tora 2002) are the key subunits of TFIID that interact with the Inr (Verrijzer et al 1994(Verrijzer et al , 1995Kaufmann et al 1998;Chalkley and Verrijzer 1999). Aside from TFIID binding to the Inr, it has also been observed that purified RNA polymerase II (or RNA polymerase II along with TBP, TFIIB, TFIIF) is able to recognize the Inr and to mediate transcription in an Inr-dependent manner in the absence of TAFs (Carcamo et al 1991;Weis and Reinberg 1997).…”

Section: The Initiator (Inr)mentioning

confidence: 99%

The RNA polymerase II core promoter: a key component in the regulation of gene expression

Butler¹,

Kadonaga²

2002

Genes Dev.

538

433

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Section: The Initiator (Inr)mentioning

confidence: 99%

The RNA polymerase II core promoter: a key component in the regulation of gene expression

Butler¹,

Kadonaga²

2002

Genes Dev.

538

433

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“…CIF150 protein shows striking homology to the Drosophila TAF II 150 protein (dTAF II 150) and some similarity to the essential yeast protein TSM-1 (18,36,47). More re-cently, essentially the same cDNA was cloned independently by Martinez and colleagues and named human TAF II 150 (hTAF II 150) (23).…”

mentioning

confidence: 99%

“…Even more surprising is the discovery that one subset of TAF II s in the TFIID complex is also an integral component of histone acetylase complexes connecting the polymerase II machinery with chromatin-modifying activities (8,27,30). A better understanding of TAF II function in core promoter recognition and activation requires additional comprehensive in vivo analysis.We reported the identification of an essential cofactor for TFIID-dependent Inr function, CIF150 (cofactor of Inr function) (17,18). CIF150 protein shows striking homology to the Drosophila TAF II 150 protein (dTAF II 150) and some similarity to the essential yeast protein 36,47).…”

mentioning

confidence: 99%

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Human Transcription Factor hTAF_II150 (CIF150) Is Involved in Transcriptional Regulation of Cell Cycle Progression

Martin

Halenbeck

Kaufmann

1999

Molecular and Cellular Biology

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Here we present evidence that CIF150 (hTAF II 150), the human homolog of Drosophila TAF II 150, plays an important and selective role in establishing gene expression patterns necessary for progression through the cell cycle. Gel filtration experiments demonstrate that CIF150 (hTAF II 150) seems to be less tightly associated with human transcription factor IID than hTAF II 130 is associated with hTAF II 250. The transient functional knockout of CIF150 (hTAF II 150) protein led to cell cycle arrest at the G 2 /M transition in mammalian cell lines. PCR display analysis with the RNA derived from CIF150-depleted cells indicated that CIF150 (hTAF II 150) is required for the transcription of only a subset of RNA polymerase II genes. CIF150 (hTAF II 150) directly stimulated cyclin B1 and cyclin A transcription in cotransfection assays and in vitro assays, suggesting that the expression of these genes is dependent on CIF150 (hTAF II 150) function. We defined a CIF150 (hTAF II 150) consensus binding site and demonstrated that a CIF150-responsive cis element is present in the cyclin B1 core promoter. These results suggest that one function of CIF150 (hTAF II 150) is to select specific RNA polymerase II core promoter elements involved in cell cycle progression.The regulation of cell growth at the molecular level is a central problem in cancer biology research. Tumor cells have established different mechanisms to bypass the normal regulation of cellular proliferation including defects in cell cycle checkpoints. The eukaryotic cell cycle is composed of four distinct stages: G 1 , S, G 2 , and M (28). The transitions between cell cycle stages are partly controlled by cyclins, regulatory subunits of the cyclin-dependent kinases (34). The gene expression of cyclins itself is cell cycle regulated (21, 33) and is crucial for maintaining normal cellular growth; the deregulation of cyclin A and cyclin D1 has been correlated with different human tumors (3, 13). Despite our extensive knowledge of cyclin-dependent signaling pathways, the molecular mechanisms for the periodic expression of the cyclins are not well understood.The binding of general transcription factor IID (TFIID) to core promoter elements is considered to initiate the formation of the RNA polymerase II preinitiation complex (reviewed in references 20, 32, 37, and 49). The TATA box, the initiator (Inr) sequence, a downstream promoter element (DPE), and a TFIIB-specific sequence have been identified as core promoter cis elements (4, 5, 19; for a review, see reference 43). For promoters that contain a TATA box the core promoter recognition is carried out by the TATA-binding protein (TBP). However, many of the cyclin core promoters do not contain a functional TATA box and are lacking defined transcriptional start sites. In vitro binding studies have suggested that TBPassociated factors (TAF II s), which are subunits of the TFIID complex, play a critical role in selecting core promoters (4,5,16,22,35,47,48). Recently, a new TAF II -containing complex lacking TBP has been des...

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“…In mammalian promoters, the Inr consensus sequence is Py-Py-A ϩ1 -N-T/APy-Py (where A ϩ1 is the transcription start site) (3,20,39), whereas in Drosophila promoters, the Inr consensus is T-C-A ϩ1 -G/T-T-T/C (1,18,32). It has been found that TAF II 150 and TAF II 250 play a role in the binding of TFIID to Inr elements (8,16,21,42,44).…”

mentioning

confidence: 99%

The Downstream Promoter Element DPE Appears To Be as Widely Used as the TATA Box in Drosophila Core Promoters

Kutach

Kadonaga

2000

Molecular and Cellular Biology

312

330

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The downstream promoter element (DPE) functions cooperatively with the initiator (Inr) for the binding of TFIID in the transcription of core promoters in the absence of a TATA box. We examined the properties of sequences that can function as a DPE as well as the range of promoters that use the DPE as a core promoter element. By using an in vitro transcription assay, we identified 17 new DPE-dependent promoters and found that all possessed identical spacing between the Inr and DPE. Moreover, mutational analysis indicated that the insertion or deletion of a single nucleotide between the Inr and DPE causes a reduction in transcriptional activity and TFIID binding. To explore the range of sequences that can function as a DPE, we constructed and analyzed randomized promoter libraries. These experiments yielded the DPE functional range set, which represents sequences that contribute to or are compatible with DPE function. We then analyzed the DPE functional range set in conjunction with a Drosophila core promoter database that we compiled from 205 promoters with accurately mapped start sites. Somewhat surprisingly, the DPE sequence motif is as common as the TATA box in Drosophila promoters. There is, in addition, a striking adherence of Inr sequences to the Inr consensus in DPE-containing promoters relative to DPE-less promoters. Furthermore, statistical and biochemical analyses indicated that a G nucleotide between the Inr and DPE contributes to transcription from DPE-containing promoters. Thus, these data reveal that the DPE exhibits a strict spacing requirement yet some sequence flexibility and appears to be as widely used as the TATA box in Drosophila.

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CIF150, a Human Cofactor for Transcription Factor IID-Dependent Initiator Function

Cited by 59 publications

References 38 publications

The RNA polymerase II core promoter: a key component in the regulation of gene expression

The RNA polymerase II core promoter: a key component in the regulation of gene expression

Human Transcription Factor hTAF_II150 (CIF150) Is Involved in Transcriptional Regulation of Cell Cycle Progression

The Downstream Promoter Element DPE Appears To Be as Widely Used as the TATA Box in Drosophila Core Promoters

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CIF150, a Human Cofactor for Transcription Factor IID-Dependent Initiator Function

Cited by 59 publications

References 38 publications

The RNA polymerase II core promoter: a key component in the regulation of gene expression

The RNA polymerase II core promoter: a key component in the regulation of gene expression

Human Transcription Factor hTAFII150 (CIF150) Is Involved in Transcriptional Regulation of Cell Cycle Progression

The Downstream Promoter Element DPE Appears To Be as Widely Used as the TATA Box in Drosophila Core Promoters

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Human Transcription Factor hTAF_II150 (CIF150) Is Involved in Transcriptional Regulation of Cell Cycle Progression