Çiğ Tavuk Etlerinden İzole Edilen Termofilik Campylobacter İzolatlarının Prevalansı ve Antimikrobiyal Direnci

Abstract

Globally, the spread of antibiotic resistance via chicken meat consumption cause serious public health concerns. With this respect, the current study aimed to investigate the prevalence of thermophilic Campylobacter species isolated from raw meat chicken samples and their genetic determinants of resistance to various classes of antibiotics. A total of 540 chicken raw meat samples collected from various supermarkets and slaughterhouses in Istanbul, Turkey were analyzed according to EN ISO 10272-1:2006 standard procedure. For identification of the genus and species of the isolates, multiplex PCR assay was held. Minimum inhibitory concentrations of the antimicrobial agents (nalidixic acid, ciprofloxacin, tetracycline, gentamicin, kanamycin, and erythromycin) were initially determined using the broth microdilution method. In addition, the genetic determinants of antimicrobial resistance were investigated by PCR assays. In total, 357 (66.1%) Campylobacter isolates were obtained including 268 Campylobacter jejuni and 89 Campylobacter coli. Resistance to quinolones (nalidixic acid and ciprofloxacin) was the most common in all strains (80.1%), followed by resistance to tetracycline's (70.3%). The lowest resistance was determined as resistance to kanamycin (4.2%). Gentamicin and erythromycin resistance was not observed in this study. Only five C. coli isolate (1.4%) was classified as multidrug resistant. On the basis of these data, execute widely presence of antimicrobial resistance to quinolones and tetracycline's in C. jejuni and C. coli isolates from chicken raw meat samples and emphasizes that further multidisciplinary studies and novel strategies in the concept of 'One Health' are needed.

Prevalence and antimicrobial resistance of Campylobacter species in shelter‐housed healthy and diarrheic cats and dogs in Turkey

“…(Aslantaş, 2019) and also in studies from other countries; for example, 18.2% in a study from Northern Taiwan (Tsai et al, 2007) and 45.5% and 75%, respectively, from two studies in Iran (Torkan et al, 2018). Quinolone resistance has also been reported in previous studies investigating Campylobacter carriage in humans (Abay et al, 2014a), chickens (Adıgüzel et al, 2018) and chicken meat (Issa et al, 2018) in Turkey. Since ciprofloxacin is the preferred antibiotic for the treatment of campylobacteriosis in humans, resistance to this antibiotic is of concern and poses a risk to public health, especially considering the frequent contact of cats and dogs with humans (Zhang et al, 2023).…”

“…In this study, a total of 26 thermophilic Campylobacter isolates (25 C. Studies on Campylobacter in Turkey have generally been carried out on poultry and cattle (Adıgüzel et al, 2018;Issa et al, 2018;Abay et al, 2014a), with only a few studies having investigated Campylobacter spp. carriage in cats and dogs (Aslantaş, 2019;Aydin et al, 2007).…”

“…In contrast to other studies, the phenotypically tetracycline-and gentamicin-resistant strains did not carry the tetO or aphA-3 genes, respectively. Previous studies have reported that both C. jejuni and C. coli isolates can carry the tetracycline resistance gene tetO (Adıgüzel et al, 2018;Aslantaş, 2019;Issa et al, 2018) and another study reported the presence of tetO and tetA genes in Campylobacter spp. isolated from poultry carcasses in Iran (Abdi-Hachesoo et al, 2014).…”