Cigarette heavy smoking alters DNA methylation patterns and gene transcription levels in humans spermatozoa

Laqqan, Mohammed M.; Yassin, Maged M.

doi:10.1007/s11356-021-17786-8

Cited by 15 publications

(6 citation statements)

References 65 publications

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“…Other studies demonstrated a significant decline in the transcription level of PRM1 and PRM2 in smokers spermatozoa compared to non-smokers [36], and that reactive oxygen species (ROS) resulting from smoking might be responsible for the decrease of the transcription level of genes like IκBα [37]. Another study showed a downregulation in the transcription levels of MAPK8IP3, GAA, ANXA2, PRRC2A, and PDE11A genes in heavy smokers compared to non-smokers and an upregulation in the MAPK8Ip3, ANXA2, PRRC2A, and PDE11A transcription levels in heavy smokers compared to non-smokers [38].…”

Section: Discussionmentioning

confidence: 99%

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Impact of Heavy Smoking on the Expression Levels of PGAM5, PTPRN2 and TYRO3 and Its Correlation to Male Infertility

H¹,

Alkhaled²,

Bibi³

et al. 2023

Preprint

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Smoking have been linked to male infertility by affecting the sperm epigenome and genome. In this study, we aimed to determine possible changes in the transcript levels of the PTPRN2, PGAM5, and TYRO3 genes in heavy smokers compared with non-smokers; and to investigate their association with fundamental sperm parameters. One hundred eighteen sperm samples (63 heavy-smokers (G1) and 55 non-smokers (G2) were included in this study. Semen analysis was performed according to WHO guidelines. After total RNA extraction, RT-PCR was used to quantify the transcript levels of studied genes. In G1 a significant decrease in standard semen parameters in comparison to non-smokers has been shown (p&lt;0.05). Moreover, PGAM5 and PTPRN2 were differentially expressed (P≤0.03 and P≤0.01, respectively), and downregulated in spermatozoa of G1 compared to G2. In contrast, no difference was observed for TYRO3 (p≤0.3). In G1, mRNA expression level of studied genes correlated negatively with motility, sperm count, normal form, vitality, and sperm membrane integrity (p&lt;0.05). Therefore, smoking may affect gene expression and male fertility by altering DNA methylation patterns in genes associated with fertility and sperm quality, including PGAM5, PTPRN2, and TYRO3.

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Section: Discussionmentioning

confidence: 99%

“…Total sperm count, total motility, progressive motility, normal form, vitality, and sperm membrane integrity were significantly higher in non-smokers group in comparison to heavy smokers group: 52. 38…”

Section: Characteristics Of the Study Populationmentioning

confidence: 99%

Impact of Heavy Smoking on the Expression Levels of PGAM5, PTPRN2 and TYRO3 and Its Correlation to Male Infertility

H¹,

Alkhaled²,

Bibi³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Other studies have demonstrated a significant decline in the transcription levels of PRM1 and PRM2 in smokers’ spermatozoa compared to that of non-smokers [ 52 ], and that reactive oxygen species (ROS) resulting from smoking might be responsible for the decrease in the transcription levels of genes such as IκBα [ 53 ]. Another study showed a downregulation in the transcription levels of the MAPK8IP3 , GAA , ANXA2 , PRRC2A , and PDE11A genes in heavy smokers compared to non-smokers and an upregulation in the MAPK8Ip3 , ANXA2 , PRRC2A , and PDE11A gene transcription levels in heavy smokers compared to non-smokers [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

The Impact of Heavy Smoking on Male Infertility and Its Correlation with the Expression Levels of the PTPRN2 and PGAM5 Genes

Alkhaled

Bibi

et al. 2023

Genes

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Smoking has been linked to male infertility by affecting the sperm epigenome and genome. In this study, we aimed to determine possible changes in the transcript levels of PGAM5 (the phosphoglycerate mutase family member 5), PTPRN2 (protein tyrosine phosphatase, N2-type receptor), and TYRO3 (tyrosine protein kinase receptor) in heavy smokers compared to non-smokers, and to investigate their association with the fundamental sperm parameters. In total, 118 sperm samples (63 heavy-smokers (G1) and 55 non-smokers (G2)) were included in this study. A semen analysis was performed according to the WHO guidelines. After a total RNA extraction, RT-PCR was used to quantify the transcript levels of the studied genes. In G1, a significant decrease in the standard semen parameters in comparison to the non-smokers was shown (p < 0.05). Moreover, PGAM5 and PTPRN2 were differentially expressed (p ≤ 0.03 and p ≤ 0.01, respectively) and downregulated in the spermatozoa of G1 compared to G2. In contrast, no difference was observed for TYRO3 (p ≤ 0.3). In G1, the mRNA expression level of the studied genes was correlated negatively with motility, sperm count, normal form, vitality, and sperm membrane integrity (p < 0.05). Therefore, smoking may affect gene expression and male fertility by altering the DNA methylation patterns in the genes associated with fertility and sperm quality, including PGAM5, PTPRN2, and TYRO3.

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“…The mechanism of this phenomenon may explain the new field of epigenomic paternal transmission, which suggests that paternal exposure to environmental challenges plays a critical role in the offspring's future health and the transmission of acquired traits through generations [ 54 ]. Several studies showed that smoking alters DNA methylation patterns and gene transcription levels in human spermatozoa [ 55 , 56 ]. Paternal exposure to cigarette smoke leads to increased global methylation of sperm DNA and alterations to the differentially methylated regions of the DLK1 gene in offspring, which in turn leads to elevated liver fat accumulation and may perturb long-term metabolic function in offspring [ 57 , 58 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Exposure to Paternal Smoking on Overweight and Obesity in Children: Findings from the Children Lifeway Cohort in Shenzhen, Southern China

You¹,

Liu²,

Hua³

et al. 2022

Obes Facts

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Introduction: Paternal smoking associated with childhood overweight and obesity has been a concern, but studies have not investigated smoking exposure and smoking details. We investigated the association of exposures from paternal smoking as well as smoking details on offspring overweight/obesity. Methods: A total of 4513 children (aged 7-8 years) in Shenzhen were enrolled. Four different exposures from paternal smoking as well as smoking quantity, duration of smoking and age of starting smoking details were the exposure variables and demographic characteristics, circumstances of birth, dietary intake, lifestyle and nonpaternal smoking exposure were covariates in logistic regression analysis to determine the effect of paternal smoking on childhood overweight/obesity, estimating odds ratios (ORs) and 95% confidence intervals (CIs). Results: Paternal smoking was positively associated with childhood overweight/obesity (p<0.05). Moreover, only pre-conception exposure, and both pre- and post-conception exposure were significantly associated with childhood overweight/obesity (OR 1.54 (95% CI 1.14-2.08) and OR 1.73 (95% CI 1.14-2.61) respectively), restricted to boys but not girls. Furthermore, for children with only pre-conception paternal-smoking exposure, the dose–response relation was positive between smoking quantity, duration of smoking, age at starting and overweight/obesity for boy offspring (P trend <0.001). We did not find any significant association between only post-natal exposure to paternal smoking and childhood overweight/obesity (P>0.05). Conclusions: Our findings suggest that paternal smoking is associated with boys’ overweight/obesity, and this association may due to the paternal smoking exposure before conception rather than the post-natal exposure to paternal smoking. Reducing paternal smoking exposure before conception might help reduce overweight/obesity in boys.

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Cigarette heavy smoking alters DNA methylation patterns and gene transcription levels in humans spermatozoa

Cited by 15 publications

References 65 publications

Impact of Heavy Smoking on the Expression Levels of PGAM5, PTPRN2 and TYRO3 and Its Correlation to Male Infertility

Impact of Heavy Smoking on the Expression Levels of PGAM5, PTPRN2 and TYRO3 and Its Correlation to Male Infertility

The Impact of Heavy Smoking on Male Infertility and Its Correlation with the Expression Levels of the PTPRN2 and PGAM5 Genes

Effect of Exposure to Paternal Smoking on Overweight and Obesity in Children: Findings from the Children Lifeway Cohort in Shenzhen, Southern China

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